Limits...
Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function.

Chen Y, Li J, Li Q, Wang T, Xing L, Xu H, Wang Y, Shi Q, Zhou Q, Liang Q - Evid Based Complement Alternat Med (2016)

Bottom Line: After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad.And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct.In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

ABSTRACT
To investigate whether Du-Huo-Ji-Sheng-Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3-month-old TNF-Tg mice with DHJST (12 g/kg) or the same volume of physiological saline once every day for 12 weeks, and 3-month-old WT littermates were used as negative control. After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad. Histology staining at ankle joints showed that DHJST decreases synovial inflammation, bone erosion, cartilage erosion, and TRAP+ osteoclast area in TNF-Tg mice. Immunohistochemical staining by using anti-Lyve-1 and anti-podoplanin antibody showed that DHJST stimulated lymphangiogenesis in ankle joints of TNF-Tg mice. And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct. In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

No MeSH data available.


Related in: MedlinePlus

DHJST increases lymphangiogenesis in ankle joints of TNF-Tg mice. (a) Three-month DHJST or saline treatment, ankle joints were harvested and subjected to double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies. Representative LYVE-1 (green) and podoplanin (red) stained ankle sections show that LYVE-1+/podoplanin+ lymphatic vessels (white arrows) are present at synovium and soft tissue surrounding the ankle joints of WT and TNF-Tg mice. (b) Quantitation of LYVE-1+/podoplanin+ lymphatic vessel area inside the areas of ankle joint. Values are the means ± SD of 5-6 legs per group. ∗p < 0.05 versus WT group, #p < 0.05 versus saline treated TNF-Tg group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4863122&req=5

fig5: DHJST increases lymphangiogenesis in ankle joints of TNF-Tg mice. (a) Three-month DHJST or saline treatment, ankle joints were harvested and subjected to double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies. Representative LYVE-1 (green) and podoplanin (red) stained ankle sections show that LYVE-1+/podoplanin+ lymphatic vessels (white arrows) are present at synovium and soft tissue surrounding the ankle joints of WT and TNF-Tg mice. (b) Quantitation of LYVE-1+/podoplanin+ lymphatic vessel area inside the areas of ankle joint. Values are the means ± SD of 5-6 legs per group. ∗p < 0.05 versus WT group, #p < 0.05 versus saline treated TNF-Tg group.

Mentions: Previously we reported that TNF-Tg mice develop ankle synovitis and significantly increased numbers of LYVE-1+ lymphatic vessels [4]. To investigate the effect of DHJST on lymphatic formation at ankle joint of TNF-Tg mice and avoid nonspecific staining, we performed double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies to visualize lymphatic vessel endothelial cells. We found a similar increase of podoplanin+/LYVE-1+ lymphatic vessels in saline treated TNF-Tg mice, compared with WT littermates. Interestingly, we observed that DHJST treatment significantly increased podoplanin+/LYVE-1+ lymphatic vessels of TNF-Tg mice (Figures 5(a) and 5(b)).


Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function.

Chen Y, Li J, Li Q, Wang T, Xing L, Xu H, Wang Y, Shi Q, Zhou Q, Liang Q - Evid Based Complement Alternat Med (2016)

DHJST increases lymphangiogenesis in ankle joints of TNF-Tg mice. (a) Three-month DHJST or saline treatment, ankle joints were harvested and subjected to double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies. Representative LYVE-1 (green) and podoplanin (red) stained ankle sections show that LYVE-1+/podoplanin+ lymphatic vessels (white arrows) are present at synovium and soft tissue surrounding the ankle joints of WT and TNF-Tg mice. (b) Quantitation of LYVE-1+/podoplanin+ lymphatic vessel area inside the areas of ankle joint. Values are the means ± SD of 5-6 legs per group. ∗p < 0.05 versus WT group, #p < 0.05 versus saline treated TNF-Tg group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863122&req=5

fig5: DHJST increases lymphangiogenesis in ankle joints of TNF-Tg mice. (a) Three-month DHJST or saline treatment, ankle joints were harvested and subjected to double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies. Representative LYVE-1 (green) and podoplanin (red) stained ankle sections show that LYVE-1+/podoplanin+ lymphatic vessels (white arrows) are present at synovium and soft tissue surrounding the ankle joints of WT and TNF-Tg mice. (b) Quantitation of LYVE-1+/podoplanin+ lymphatic vessel area inside the areas of ankle joint. Values are the means ± SD of 5-6 legs per group. ∗p < 0.05 versus WT group, #p < 0.05 versus saline treated TNF-Tg group.
Mentions: Previously we reported that TNF-Tg mice develop ankle synovitis and significantly increased numbers of LYVE-1+ lymphatic vessels [4]. To investigate the effect of DHJST on lymphatic formation at ankle joint of TNF-Tg mice and avoid nonspecific staining, we performed double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies to visualize lymphatic vessel endothelial cells. We found a similar increase of podoplanin+/LYVE-1+ lymphatic vessels in saline treated TNF-Tg mice, compared with WT littermates. Interestingly, we observed that DHJST treatment significantly increased podoplanin+/LYVE-1+ lymphatic vessels of TNF-Tg mice (Figures 5(a) and 5(b)).

Bottom Line: After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad.And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct.In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

ABSTRACT
To investigate whether Du-Huo-Ji-Sheng-Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3-month-old TNF-Tg mice with DHJST (12 g/kg) or the same volume of physiological saline once every day for 12 weeks, and 3-month-old WT littermates were used as negative control. After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad. Histology staining at ankle joints showed that DHJST decreases synovial inflammation, bone erosion, cartilage erosion, and TRAP+ osteoclast area in TNF-Tg mice. Immunohistochemical staining by using anti-Lyve-1 and anti-podoplanin antibody showed that DHJST stimulated lymphangiogenesis in ankle joints of TNF-Tg mice. And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct. In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

No MeSH data available.


Related in: MedlinePlus