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Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function.

Chen Y, Li J, Li Q, Wang T, Xing L, Xu H, Wang Y, Shi Q, Zhou Q, Liang Q - Evid Based Complement Alternat Med (2016)

Bottom Line: After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad.And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct.In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

ABSTRACT
To investigate whether Du-Huo-Ji-Sheng-Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3-month-old TNF-Tg mice with DHJST (12 g/kg) or the same volume of physiological saline once every day for 12 weeks, and 3-month-old WT littermates were used as negative control. After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad. Histology staining at ankle joints showed that DHJST decreases synovial inflammation, bone erosion, cartilage erosion, and TRAP+ osteoclast area in TNF-Tg mice. Immunohistochemical staining by using anti-Lyve-1 and anti-podoplanin antibody showed that DHJST stimulated lymphangiogenesis in ankle joints of TNF-Tg mice. And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct. In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

No MeSH data available.


Related in: MedlinePlus

Du-Huo-Ji-Sheng-Tang reduces TRAP+ osteoclast in TNF-Tg mice. (a) Representative TRAP-stained sections show decreased TRAP+ osteoclast number in the Du-Huo-Ji-Sheng-Tang-treated joints. Bar, 200 μm, arrow indicates TRAP+ osteoclast. Quantitation of TRAP+ osteoclast area (b). Values are the mean ± SD of 6–8 legs per group. ∗p < 0.05 versus WT; #p < 0.05 versus TNF-Tg + saline.
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fig3: Du-Huo-Ji-Sheng-Tang reduces TRAP+ osteoclast in TNF-Tg mice. (a) Representative TRAP-stained sections show decreased TRAP+ osteoclast number in the Du-Huo-Ji-Sheng-Tang-treated joints. Bar, 200 μm, arrow indicates TRAP+ osteoclast. Quantitation of TRAP+ osteoclast area (b). Values are the mean ± SD of 6–8 legs per group. ∗p < 0.05 versus WT; #p < 0.05 versus TNF-Tg + saline.

Mentions: To investigate the effect of DHJST on arthritis, TNF-Tg mice were given DHJST by gavage daily for 12 weeks. We quantified synovial inflammation and bone erosion in HE staining sections of ankle joints, cartilage damage in ABHO staining sections, and osteoclast invasion in TRAP staining section. Synovial volume in ankle joint of WT mice is 0.015 ± 0.0044 mm2, while severely increased synovial inflammation volume was observed in saline treated TNF-Tg mice (1.767 ± 0.69 mm2). Impressively, DHJST treatment significantly reduced inflammatory synovial volume of TNF-Tg mice, versus saline treated control (Figures 1(a) and 1(b)). In addition, significantly reduced bone area was observed in saline treated TNF-Tg mice, versus WT littermates. However DHJST treatment restored the bone volume of TNF-Tg mice, compared with saline treated group (Figures 1(a) and 1(c)). Besides that, the cartilage area is also significantly reduced in saline treated TNF-Tg mice versus WT mice. Compared with saline treated TNF-Tg mice, DHJST significantly increased cartilage area of TNF-Tg mice (Figures 2(a) and 2(b)). Furthermore, severely increased osteoclast area was observed in ankle joints of saline treated TNF-Tg mice, which was significantly reduced by DHJST treatment (Figures 3(a) and 3(b)).


Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function.

Chen Y, Li J, Li Q, Wang T, Xing L, Xu H, Wang Y, Shi Q, Zhou Q, Liang Q - Evid Based Complement Alternat Med (2016)

Du-Huo-Ji-Sheng-Tang reduces TRAP+ osteoclast in TNF-Tg mice. (a) Representative TRAP-stained sections show decreased TRAP+ osteoclast number in the Du-Huo-Ji-Sheng-Tang-treated joints. Bar, 200 μm, arrow indicates TRAP+ osteoclast. Quantitation of TRAP+ osteoclast area (b). Values are the mean ± SD of 6–8 legs per group. ∗p < 0.05 versus WT; #p < 0.05 versus TNF-Tg + saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Du-Huo-Ji-Sheng-Tang reduces TRAP+ osteoclast in TNF-Tg mice. (a) Representative TRAP-stained sections show decreased TRAP+ osteoclast number in the Du-Huo-Ji-Sheng-Tang-treated joints. Bar, 200 μm, arrow indicates TRAP+ osteoclast. Quantitation of TRAP+ osteoclast area (b). Values are the mean ± SD of 6–8 legs per group. ∗p < 0.05 versus WT; #p < 0.05 versus TNF-Tg + saline.
Mentions: To investigate the effect of DHJST on arthritis, TNF-Tg mice were given DHJST by gavage daily for 12 weeks. We quantified synovial inflammation and bone erosion in HE staining sections of ankle joints, cartilage damage in ABHO staining sections, and osteoclast invasion in TRAP staining section. Synovial volume in ankle joint of WT mice is 0.015 ± 0.0044 mm2, while severely increased synovial inflammation volume was observed in saline treated TNF-Tg mice (1.767 ± 0.69 mm2). Impressively, DHJST treatment significantly reduced inflammatory synovial volume of TNF-Tg mice, versus saline treated control (Figures 1(a) and 1(b)). In addition, significantly reduced bone area was observed in saline treated TNF-Tg mice, versus WT littermates. However DHJST treatment restored the bone volume of TNF-Tg mice, compared with saline treated group (Figures 1(a) and 1(c)). Besides that, the cartilage area is also significantly reduced in saline treated TNF-Tg mice versus WT mice. Compared with saline treated TNF-Tg mice, DHJST significantly increased cartilage area of TNF-Tg mice (Figures 2(a) and 2(b)). Furthermore, severely increased osteoclast area was observed in ankle joints of saline treated TNF-Tg mice, which was significantly reduced by DHJST treatment (Figures 3(a) and 3(b)).

Bottom Line: After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad.And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct.In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

ABSTRACT
To investigate whether Du-Huo-Ji-Sheng-Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3-month-old TNF-Tg mice with DHJST (12 g/kg) or the same volume of physiological saline once every day for 12 weeks, and 3-month-old WT littermates were used as negative control. After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad. Histology staining at ankle joints showed that DHJST decreases synovial inflammation, bone erosion, cartilage erosion, and TRAP+ osteoclast area in TNF-Tg mice. Immunohistochemical staining by using anti-Lyve-1 and anti-podoplanin antibody showed that DHJST stimulated lymphangiogenesis in ankle joints of TNF-Tg mice. And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct. In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.

No MeSH data available.


Related in: MedlinePlus