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Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Predicted mechanism in esophageal tissue on administering RC400. RC400 decreased serum ROS and ONOO− production. Further, RC400 ameliorated the values of proinflammatory mediators (COX-2 and iNOS) and cytokines (TNF-α and IL-6) regulated by NF-κB and increased oxidative defense factor SOD and HO-1 proteins.
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Related In: Results  -  Collection


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fig8: Predicted mechanism in esophageal tissue on administering RC400. RC400 decreased serum ROS and ONOO− production. Further, RC400 ameliorated the values of proinflammatory mediators (COX-2 and iNOS) and cytokines (TNF-α and IL-6) regulated by NF-κB and increased oxidative defense factor SOD and HO-1 proteins.

Mentions: A recent study has shown that ROS are one of the potent factors in the pathogenesis of esophageal mucosal damage mediated by oxidative stress in an experimental model of reflux esophagitis [55]. In the present study, the administration of RC-mix reduced the oxidative stress biomarker and increased the activities of SOD and HO-1. Furthermore, the anti-inflammatory effects of RC-mix suggested that the inactivation of NF-κB by blocking the phosphorylation of IκBα led to the inhibition of the release of proinflammatory cytokines and mediators, reducing the inflammatory damage that is typical in the esophageal mucosa of rats with reflux esophagitis (Figure 8).


Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

Predicted mechanism in esophageal tissue on administering RC400. RC400 decreased serum ROS and ONOO− production. Further, RC400 ameliorated the values of proinflammatory mediators (COX-2 and iNOS) and cytokines (TNF-α and IL-6) regulated by NF-κB and increased oxidative defense factor SOD and HO-1 proteins.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863117&req=5

fig8: Predicted mechanism in esophageal tissue on administering RC400. RC400 decreased serum ROS and ONOO− production. Further, RC400 ameliorated the values of proinflammatory mediators (COX-2 and iNOS) and cytokines (TNF-α and IL-6) regulated by NF-κB and increased oxidative defense factor SOD and HO-1 proteins.
Mentions: A recent study has shown that ROS are one of the potent factors in the pathogenesis of esophageal mucosal damage mediated by oxidative stress in an experimental model of reflux esophagitis [55]. In the present study, the administration of RC-mix reduced the oxidative stress biomarker and increased the activities of SOD and HO-1. Furthermore, the anti-inflammatory effects of RC-mix suggested that the inactivation of NF-κB by blocking the phosphorylation of IκBα led to the inhibition of the release of proinflammatory cytokines and mediators, reducing the inflammatory damage that is typical in the esophageal mucosa of rats with reflux esophagitis (Figure 8).

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus