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Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Esophageal COX-2 (a), iNOS (b), TNF-α (c), and IL-6 (d) protein expressions. N, normal rats; Veh, positive control rats with reflux esophagitis (RE); RC100, RC200, and RC400 RE rats, animals treated with RC-mix 100 mg/kg, RC-mix 200 mg/kg, and RC-mix 400 mg/kg body weight, respectively. Data are the means ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus RE control rat values. n = 6 in each group.
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fig7: Esophageal COX-2 (a), iNOS (b), TNF-α (c), and IL-6 (d) protein expressions. N, normal rats; Veh, positive control rats with reflux esophagitis (RE); RC100, RC200, and RC400 RE rats, animals treated with RC-mix 100 mg/kg, RC-mix 200 mg/kg, and RC-mix 400 mg/kg body weight, respectively. Data are the means ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus RE control rat values. n = 6 in each group.

Mentions: The quantified COX-2, iNOS, TNF-α, and IL-6 protein expressions are shown in Figures 7(a), 7(b), 7(c), and 7(d), respectively. The inflammation-related protein expressions in the RE control rats were significantly augmented in the esophagus compared with normal rats. However, treatment with RC-mix suppressed these proteins in the esophagus. Moreover, COX-2 and IL-6 decreased significantly in all RC experimental rats and, above all, the administration of RC400 reduced nearly to normal levels or below in the esophagus. iNOS and TNF-α protein expression exhibited no significant changes in RC100 and RC200 but RC400 reduced significantly.


Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

Esophageal COX-2 (a), iNOS (b), TNF-α (c), and IL-6 (d) protein expressions. N, normal rats; Veh, positive control rats with reflux esophagitis (RE); RC100, RC200, and RC400 RE rats, animals treated with RC-mix 100 mg/kg, RC-mix 200 mg/kg, and RC-mix 400 mg/kg body weight, respectively. Data are the means ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus RE control rat values. n = 6 in each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863117&req=5

fig7: Esophageal COX-2 (a), iNOS (b), TNF-α (c), and IL-6 (d) protein expressions. N, normal rats; Veh, positive control rats with reflux esophagitis (RE); RC100, RC200, and RC400 RE rats, animals treated with RC-mix 100 mg/kg, RC-mix 200 mg/kg, and RC-mix 400 mg/kg body weight, respectively. Data are the means ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus RE control rat values. n = 6 in each group.
Mentions: The quantified COX-2, iNOS, TNF-α, and IL-6 protein expressions are shown in Figures 7(a), 7(b), 7(c), and 7(d), respectively. The inflammation-related protein expressions in the RE control rats were significantly augmented in the esophagus compared with normal rats. However, treatment with RC-mix suppressed these proteins in the esophagus. Moreover, COX-2 and IL-6 decreased significantly in all RC experimental rats and, above all, the administration of RC400 reduced nearly to normal levels or below in the esophagus. iNOS and TNF-α protein expression exhibited no significant changes in RC100 and RC200 but RC400 reduced significantly.

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus