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Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus

DPPH radical scavenging activity (a) and ABTS radical scavenging activity (b) of RC-mix, water extract of Rhei rhizoma and Coptis rhizoma mixture. Each experiment was run in triplicate.
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fig2: DPPH radical scavenging activity (a) and ABTS radical scavenging activity (b) of RC-mix, water extract of Rhei rhizoma and Coptis rhizoma mixture. Each experiment was run in triplicate.

Mentions: In this study, DPPH and ABTS radical scavenging activity were performed to determine and confirm antioxidant activity of RC-mix. IC50 (μg/mL) represents half maximal concentration of tested compounds to scavenge DPPH and ABTS radical. As shown in Figure 2(a), the IC50 of DPPH radical scavenging activity of RC-mix was found at 17.74 ± 2.14 μg/mL and the IC50 value of ascorbic acid (positive control) as a positive control was 1.28 ± 0.04 μg/mL. The calculated IC50 value of RC-mix against the ABTS+ radical was determined to be 27.60 ± 0.94 μg/mL and the IC50 value of ascorbic acid (positive control) as a positive control was 2.20 ± 0.02 μg/mL (Figure 2(b)).


Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

DPPH radical scavenging activity (a) and ABTS radical scavenging activity (b) of RC-mix, water extract of Rhei rhizoma and Coptis rhizoma mixture. Each experiment was run in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863117&req=5

fig2: DPPH radical scavenging activity (a) and ABTS radical scavenging activity (b) of RC-mix, water extract of Rhei rhizoma and Coptis rhizoma mixture. Each experiment was run in triplicate.
Mentions: In this study, DPPH and ABTS radical scavenging activity were performed to determine and confirm antioxidant activity of RC-mix. IC50 (μg/mL) represents half maximal concentration of tested compounds to scavenge DPPH and ABTS radical. As shown in Figure 2(a), the IC50 of DPPH radical scavenging activity of RC-mix was found at 17.74 ± 2.14 μg/mL and the IC50 value of ascorbic acid (positive control) as a positive control was 1.28 ± 0.04 μg/mL. The calculated IC50 value of RC-mix against the ABTS+ radical was determined to be 27.60 ± 0.94 μg/mL and the IC50 value of ascorbic acid (positive control) as a positive control was 2.20 ± 0.02 μg/mL (Figure 2(b)).

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus