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Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatogram of RC-mix extract (1 mg/mL) detected 254 nm. Signals 1–5 identified to be sennoside A, epiberberine, coptisine, palmatine, and berberine in regular sequence. RC-mix, a water extract of Rhei rhizoma and Coptis rhizoma mixture.
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fig1: HPLC chromatogram of RC-mix extract (1 mg/mL) detected 254 nm. Signals 1–5 identified to be sennoside A, epiberberine, coptisine, palmatine, and berberine in regular sequence. RC-mix, a water extract of Rhei rhizoma and Coptis rhizoma mixture.

Mentions: The water extract of Rhei rhizoma and Coptidis rhizoma mixture (1 mg) was dissolved in 1 mL of 50% methanol with multi-vortexing. We injected 50 μL of the sample into a reverse-phase HPLC using a ZORBAX Eclipse XDB-C18, analytical 4.6 × 150 mm, 5 microns, with a column temperature of 25°C. Mobile phase component A is methanol and B is water (10 mM 1-hexanesulfonic acid sodium). The gradient conditions were as follows: 15% A, 0 min, 50% A, 15 min, and 30% A, 30 min. The flow rate was 2.0 mL/min. The UV absorbance from 254 nm was monitored using an Agilent 1200 series with an 2998 Photodiode Array Detector from Waters Co. (Manchester, UK). All peaks were assigned by carrying out coinjection tests with authentic samples and comparing them with the UV spectral data. The major components of Rhei rhizoma and Coptidis rhizoma were sennoside A, epiberberine, coptisine, palmatine, and berberine. Sennoside A was detected from Rhei rhizoma and epiberberine, coptisine, palmatine, and berberine were detected from Coptidis rhizoma. The measurement was repeated three times. Representative HPLC results are illustrated in Figure 1.


Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.

Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, Shin MR, Choi HG, Kim JA, Min BS, Kim GN, Noh JS, Rhee MH, Roh SS - Evid Based Complement Alternat Med (2016)

HPLC chromatogram of RC-mix extract (1 mg/mL) detected 254 nm. Signals 1–5 identified to be sennoside A, epiberberine, coptisine, palmatine, and berberine in regular sequence. RC-mix, a water extract of Rhei rhizoma and Coptis rhizoma mixture.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863117&req=5

fig1: HPLC chromatogram of RC-mix extract (1 mg/mL) detected 254 nm. Signals 1–5 identified to be sennoside A, epiberberine, coptisine, palmatine, and berberine in regular sequence. RC-mix, a water extract of Rhei rhizoma and Coptis rhizoma mixture.
Mentions: The water extract of Rhei rhizoma and Coptidis rhizoma mixture (1 mg) was dissolved in 1 mL of 50% methanol with multi-vortexing. We injected 50 μL of the sample into a reverse-phase HPLC using a ZORBAX Eclipse XDB-C18, analytical 4.6 × 150 mm, 5 microns, with a column temperature of 25°C. Mobile phase component A is methanol and B is water (10 mM 1-hexanesulfonic acid sodium). The gradient conditions were as follows: 15% A, 0 min, 50% A, 15 min, and 30% A, 30 min. The flow rate was 2.0 mL/min. The UV absorbance from 254 nm was monitored using an Agilent 1200 series with an 2998 Photodiode Array Detector from Waters Co. (Manchester, UK). All peaks were assigned by carrying out coinjection tests with authentic samples and comparing them with the UV spectral data. The major components of Rhei rhizoma and Coptidis rhizoma were sennoside A, epiberberine, coptisine, palmatine, and berberine. Sennoside A was detected from Rhei rhizoma and epiberberine, coptisine, palmatine, and berberine were detected from Coptidis rhizoma. The measurement was repeated three times. Representative HPLC results are illustrated in Figure 1.

Bottom Line: The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner.Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)).The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix.

View Article: PubMed Central - PubMed

Affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.

ABSTRACT
The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.

No MeSH data available.


Related in: MedlinePlus