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Cortex phellodendri Extract Relaxes Airway Smooth Muscle.

Jiang QJ, Chen W, Dan H, Tan L, Zhu H, Yang G, Shen J, Peng YB, Zhao P, Xue L, Yu MF, Ma L, Si XT, Wang Z, Dai J, Qin G, Zou C, Liu QH - Evid Based Complement Alternat Med (2016)

Bottom Line: Cortex phellodendri is used to reduce fever and remove dampness and toxin.The ACH-induced contraction was partially inhibited by nifedipine and pyrazole 3, an inhibitor of TRPC3 and STIM/Orai channels.Taken together, our data demonstrate that NBAECP can relax ASM by inhibiting L-type Ca(2+) channels and TRPC3 and/or STIM/Orai channels, suggesting that NBAECP could be developed to a new drug for relieving bronchospasm.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China.

ABSTRACT
Cortex phellodendri is used to reduce fever and remove dampness and toxin. Berberine is an active ingredient of C. phellodendri. Berberine from Argemone ochroleuca can relax airway smooth muscle (ASM); however, whether the nonberberine component of C. phellodendri has similar relaxant action was unclear. An n-butyl alcohol extract of C. phellodendri (NBAECP, nonberberine component) was prepared, which completely inhibits high K(+)- and acetylcholine- (ACH-) induced precontraction of airway smooth muscle in tracheal rings and lung slices from control and asthmatic mice, respectively. The contraction induced by high K(+) was also blocked by nifedipine, a selective blocker of L-type Ca(2+) channels. The ACH-induced contraction was partially inhibited by nifedipine and pyrazole 3, an inhibitor of TRPC3 and STIM/Orai channels. Taken together, our data demonstrate that NBAECP can relax ASM by inhibiting L-type Ca(2+) channels and TRPC3 and/or STIM/Orai channels, suggesting that NBAECP could be developed to a new drug for relieving bronchospasm.

No MeSH data available.


Related in: MedlinePlus

NBAECP inhibits ACH-induced precontraction in TRs. (a) Following cumulative addition of ACH, a TR reached a sustained contraction, which was inhibited following cumulative application of NBAECP. (b) A similar experiment was performed in asthmatic TR. (c) The summary results of NBAECP-induced relaxation in 7 control and 6 asthmatic TRs. The IC50 of NBAECP was 12.2 ± 1.3 μg/mL in control TRs and 12.8 ± 1.2 μg/mL in asthmatic TRs. These results indicated that NBAECP could block ACH-induced sustained contractions in control and asthmatic tracheal smooth muscle.
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fig2: NBAECP inhibits ACH-induced precontraction in TRs. (a) Following cumulative addition of ACH, a TR reached a sustained contraction, which was inhibited following cumulative application of NBAECP. (b) A similar experiment was performed in asthmatic TR. (c) The summary results of NBAECP-induced relaxation in 7 control and 6 asthmatic TRs. The IC50 of NBAECP was 12.2 ± 1.3 μg/mL in control TRs and 12.8 ± 1.2 μg/mL in asthmatic TRs. These results indicated that NBAECP could block ACH-induced sustained contractions in control and asthmatic tracheal smooth muscle.

Mentions: To know whether NBAECP is capable of inhibiting another agonist-induced precontraction in ASM, healthy TRs were contracted using ACH. Upon the contraction reaching the maximum, NBAECP was added (Figure 2(a)). Similar dose-dependent relaxation responses occurred. Moreover, these responses existed in asthmatic TRs (Figure 2(b)). The dose-relaxation relationships and IC50 values of NBAECP were analyzed (Figure 2(c)), and they did not show differences between the control and asthmatic TRs. These experiments demonstrated that NBAECP could also inhibit ACH-induced precontraction in control and asthmatic ASM.


Cortex phellodendri Extract Relaxes Airway Smooth Muscle.

Jiang QJ, Chen W, Dan H, Tan L, Zhu H, Yang G, Shen J, Peng YB, Zhao P, Xue L, Yu MF, Ma L, Si XT, Wang Z, Dai J, Qin G, Zou C, Liu QH - Evid Based Complement Alternat Med (2016)

NBAECP inhibits ACH-induced precontraction in TRs. (a) Following cumulative addition of ACH, a TR reached a sustained contraction, which was inhibited following cumulative application of NBAECP. (b) A similar experiment was performed in asthmatic TR. (c) The summary results of NBAECP-induced relaxation in 7 control and 6 asthmatic TRs. The IC50 of NBAECP was 12.2 ± 1.3 μg/mL in control TRs and 12.8 ± 1.2 μg/mL in asthmatic TRs. These results indicated that NBAECP could block ACH-induced sustained contractions in control and asthmatic tracheal smooth muscle.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863113&req=5

fig2: NBAECP inhibits ACH-induced precontraction in TRs. (a) Following cumulative addition of ACH, a TR reached a sustained contraction, which was inhibited following cumulative application of NBAECP. (b) A similar experiment was performed in asthmatic TR. (c) The summary results of NBAECP-induced relaxation in 7 control and 6 asthmatic TRs. The IC50 of NBAECP was 12.2 ± 1.3 μg/mL in control TRs and 12.8 ± 1.2 μg/mL in asthmatic TRs. These results indicated that NBAECP could block ACH-induced sustained contractions in control and asthmatic tracheal smooth muscle.
Mentions: To know whether NBAECP is capable of inhibiting another agonist-induced precontraction in ASM, healthy TRs were contracted using ACH. Upon the contraction reaching the maximum, NBAECP was added (Figure 2(a)). Similar dose-dependent relaxation responses occurred. Moreover, these responses existed in asthmatic TRs (Figure 2(b)). The dose-relaxation relationships and IC50 values of NBAECP were analyzed (Figure 2(c)), and they did not show differences between the control and asthmatic TRs. These experiments demonstrated that NBAECP could also inhibit ACH-induced precontraction in control and asthmatic ASM.

Bottom Line: Cortex phellodendri is used to reduce fever and remove dampness and toxin.The ACH-induced contraction was partially inhibited by nifedipine and pyrazole 3, an inhibitor of TRPC3 and STIM/Orai channels.Taken together, our data demonstrate that NBAECP can relax ASM by inhibiting L-type Ca(2+) channels and TRPC3 and/or STIM/Orai channels, suggesting that NBAECP could be developed to a new drug for relieving bronchospasm.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China.

ABSTRACT
Cortex phellodendri is used to reduce fever and remove dampness and toxin. Berberine is an active ingredient of C. phellodendri. Berberine from Argemone ochroleuca can relax airway smooth muscle (ASM); however, whether the nonberberine component of C. phellodendri has similar relaxant action was unclear. An n-butyl alcohol extract of C. phellodendri (NBAECP, nonberberine component) was prepared, which completely inhibits high K(+)- and acetylcholine- (ACH-) induced precontraction of airway smooth muscle in tracheal rings and lung slices from control and asthmatic mice, respectively. The contraction induced by high K(+) was also blocked by nifedipine, a selective blocker of L-type Ca(2+) channels. The ACH-induced contraction was partially inhibited by nifedipine and pyrazole 3, an inhibitor of TRPC3 and STIM/Orai channels. Taken together, our data demonstrate that NBAECP can relax ASM by inhibiting L-type Ca(2+) channels and TRPC3 and/or STIM/Orai channels, suggesting that NBAECP could be developed to a new drug for relieving bronchospasm.

No MeSH data available.


Related in: MedlinePlus