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Antibacterial and Antimetastatic Potential of Diospyros lycioides Extract on Cervical Cancer Cells and Associated Pathogens.

Bagla VP, Lubisi VZ, Ndiitwani T, Mokgotho MP, Mampuru L, Mbazima V - Evid Based Complement Alternat Med (2016)

Bottom Line: The four extracts were tested for antibacterial activity using bioautography against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli.The leaf extract tested positive for flavonoids, tannins, and terpenoids while the four different extracts tested in the antimicrobial assay contained constituents active against one or more of the organisms tested, except E. coli.The finding demonstrates the acetone extract to contain constituents with antibacterial and antimetastatic effects on cervical cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Microbiology and Biotechnology, Faculty of Science and Agriculture, University of Limpopo, Turfloop Campus, Private Bag X1106, Sovenga, Limpopo 0727, South Africa.

ABSTRACT
Cervical cancer is among the most prevalent forms of cancer in women worldwide. Diospyros lycioides was extracted using hexane, ethyl acetate, acetone, and methanol and finger print profiles were determined. The leaf material was tested for the presence of flavonoids, tannins, saponins, terpenoids, and cardiac glycosides using standard chemical methods and the presence of flavonoids and phenolics using thin layer chromatography. The total phenolic content was determined using Folin-Ciocalteu procedure. The four extracts were tested for antibacterial activity using bioautography against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. The acetone extract with the highest number of antibacterial and antioxidant compounds was assessed for its cytotoxicity on BUD-8 cells using the real-time xCELLigence system and its potential effects on metastatic cervical cancer (HeLa) cell migration and invasion were assessed using wound healing migration and invasion assays. The leaf extract tested positive for flavonoids, tannins, and terpenoids while the four different extracts tested in the antimicrobial assay contained constituents active against one or more of the organisms tested, except E. coli. The cytotoxicity of the acetone extract in real-time was concentration-dependent with potent ability to suppress the migration and invasion of HeLa cells. The finding demonstrates the acetone extract to contain constituents with antibacterial and antimetastatic effects on cervical cancer cells.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of the acetone extract of D. lycioides on the migration of HeLa cells. Confluent monolayers of cells were scarred, treated with 0, 150, and 300 μg/mL of the extract and 7.4 μg/mL of curcumin (positive control). Wound closure was monitored microscopically at 0, 6, and 24 h and photographed under a phase-contrast microscope at 10x magnification.
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fig6: Inhibitory effect of the acetone extract of D. lycioides on the migration of HeLa cells. Confluent monolayers of cells were scarred, treated with 0, 150, and 300 μg/mL of the extract and 7.4 μg/mL of curcumin (positive control). Wound closure was monitored microscopically at 0, 6, and 24 h and photographed under a phase-contrast microscope at 10x magnification.

Mentions: Since S. aureus and E. coli are amongst the most common bacteria implicated in vaginitis and associated cellular changes of the cervix; the acetone extract containing the highest number of antibacterial and antioxidant compounds was then tested at noncytotoxic concentrations (0, 150 and 300 μg/mL) for its ability to inhibit the migration of HeLa cells. As shown in Figure 6, the extract was shown to suppress the migration of the cells in a concentration-dependent manner. Cells exposed to the acetone extract were seen to have a reduced ability to migrate and as such prevented the closure of wounds at the various concentrations and exposure times (Figure 6).


Antibacterial and Antimetastatic Potential of Diospyros lycioides Extract on Cervical Cancer Cells and Associated Pathogens.

Bagla VP, Lubisi VZ, Ndiitwani T, Mokgotho MP, Mampuru L, Mbazima V - Evid Based Complement Alternat Med (2016)

Inhibitory effect of the acetone extract of D. lycioides on the migration of HeLa cells. Confluent monolayers of cells were scarred, treated with 0, 150, and 300 μg/mL of the extract and 7.4 μg/mL of curcumin (positive control). Wound closure was monitored microscopically at 0, 6, and 24 h and photographed under a phase-contrast microscope at 10x magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863106&req=5

fig6: Inhibitory effect of the acetone extract of D. lycioides on the migration of HeLa cells. Confluent monolayers of cells were scarred, treated with 0, 150, and 300 μg/mL of the extract and 7.4 μg/mL of curcumin (positive control). Wound closure was monitored microscopically at 0, 6, and 24 h and photographed under a phase-contrast microscope at 10x magnification.
Mentions: Since S. aureus and E. coli are amongst the most common bacteria implicated in vaginitis and associated cellular changes of the cervix; the acetone extract containing the highest number of antibacterial and antioxidant compounds was then tested at noncytotoxic concentrations (0, 150 and 300 μg/mL) for its ability to inhibit the migration of HeLa cells. As shown in Figure 6, the extract was shown to suppress the migration of the cells in a concentration-dependent manner. Cells exposed to the acetone extract were seen to have a reduced ability to migrate and as such prevented the closure of wounds at the various concentrations and exposure times (Figure 6).

Bottom Line: The four extracts were tested for antibacterial activity using bioautography against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli.The leaf extract tested positive for flavonoids, tannins, and terpenoids while the four different extracts tested in the antimicrobial assay contained constituents active against one or more of the organisms tested, except E. coli.The finding demonstrates the acetone extract to contain constituents with antibacterial and antimetastatic effects on cervical cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Microbiology and Biotechnology, Faculty of Science and Agriculture, University of Limpopo, Turfloop Campus, Private Bag X1106, Sovenga, Limpopo 0727, South Africa.

ABSTRACT
Cervical cancer is among the most prevalent forms of cancer in women worldwide. Diospyros lycioides was extracted using hexane, ethyl acetate, acetone, and methanol and finger print profiles were determined. The leaf material was tested for the presence of flavonoids, tannins, saponins, terpenoids, and cardiac glycosides using standard chemical methods and the presence of flavonoids and phenolics using thin layer chromatography. The total phenolic content was determined using Folin-Ciocalteu procedure. The four extracts were tested for antibacterial activity using bioautography against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. The acetone extract with the highest number of antibacterial and antioxidant compounds was assessed for its cytotoxicity on BUD-8 cells using the real-time xCELLigence system and its potential effects on metastatic cervical cancer (HeLa) cell migration and invasion were assessed using wound healing migration and invasion assays. The leaf extract tested positive for flavonoids, tannins, and terpenoids while the four different extracts tested in the antimicrobial assay contained constituents active against one or more of the organisms tested, except E. coli. The cytotoxicity of the acetone extract in real-time was concentration-dependent with potent ability to suppress the migration and invasion of HeLa cells. The finding demonstrates the acetone extract to contain constituents with antibacterial and antimetastatic effects on cervical cancer cells.

No MeSH data available.


Related in: MedlinePlus