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Nicotinamide Mononucleotide Adenylyl Transferase 2: A Promising Diagnostic and Therapeutic Target for Colorectal Cancer.

Cui C, Qi J, Deng Q, Chen R, Zhai D, Yu J - Biomed Res Int (2016)

Bottom Line: Significant positive correlation was found between the expression of NMNAT2 and the expression of p53.However, NMNAT2 expression was not a statistically significant prognostic factor for overall survival.In conclusion, our results indicated that NMNAT2 might participate in tumorigenesis of CRC in a p53-dependent manner and NMNAT2 expression might be a potential therapeutic target for CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

ABSTRACT
Colorectal cancer (CRC) is one of the most common cancers all over the world. It is essential to search for more effective diagnostic and therapeutic methods for CRC. Abnormal nicotinamide adenine dinucleotide (NAD) metabolism has been considered as a characteristic of cancer cells. In this study, nicotinamide mononucleotide adenylyl transferases (NMNATs) as well as p53-mediated cancer signaling pathways were investigated in patients with colorectal cancer. The CRC tissues and adjacent normal tissues were obtained from 95 untreated colorectal cancer patients and were stained for expression of nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) and p53. The survival rate was analyzed by the Kaplan-Meier method and the log-rank test. The multivariate Cox proportional hazard regression analysis was conducted as well. Our data demonstrated that expression of NMNAT2 and p53 was significantly higher in CRC tissues, while NMNAT2 expression is in correlation with the invasive depth of tumors and TNM stage. Significant positive correlation was found between the expression of NMNAT2 and the expression of p53. However, NMNAT2 expression was not a statistically significant prognostic factor for overall survival. In conclusion, our results indicated that NMNAT2 might participate in tumorigenesis of CRC in a p53-dependent manner and NMNAT2 expression might be a potential therapeutic target for CRC.

No MeSH data available.


Related in: MedlinePlus

NMNAT2 immunohistochemical staining for colorectal cancer peritumoral tissues (×200). (a) Negative (intensity score 0, percentage score 0) and (b) negative (intensity score 1, percentage score 1) and (c) positive (intensity score 2, percentage score 3) and (d) positive (intensity score 3, percentage score 3).
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fig1: NMNAT2 immunohistochemical staining for colorectal cancer peritumoral tissues (×200). (a) Negative (intensity score 0, percentage score 0) and (b) negative (intensity score 1, percentage score 1) and (c) positive (intensity score 2, percentage score 3) and (d) positive (intensity score 3, percentage score 3).

Mentions: NMNAT2 protein expression was localized primarily in cytoplasm and the diffusely cytoplasmic brown-staining was observed in positive tissues as shown in Figure 1. The specificity of the anti-NMNAT2 antibody was confirmed by western blot measuring different pairs of tumor-peritumoral tissue samples of CRC (see Figure S1 in Supplementary Material available online at http://dx.doi.org/10.1155/2016/1804137). The NMNAT2 expression could be detected in 78 of 95 (82.1%) cases among CRC tissues compared to 34 of 95 (35.7%) for adjacent normal tissues, which suggested NMNAT2 was significantly upregulated in CRC (Table 1). On the other hand, p53 protein was mainly presented in nuclear of cancer cells (Figure 2). Our results showed that the protein expression of p53 in CRC tissues (60 of 95, 63.1%) was significantly more than adjacent normal tissues (16 of 95, 16.8%) (P < 0.01).


Nicotinamide Mononucleotide Adenylyl Transferase 2: A Promising Diagnostic and Therapeutic Target for Colorectal Cancer.

Cui C, Qi J, Deng Q, Chen R, Zhai D, Yu J - Biomed Res Int (2016)

NMNAT2 immunohistochemical staining for colorectal cancer peritumoral tissues (×200). (a) Negative (intensity score 0, percentage score 0) and (b) negative (intensity score 1, percentage score 1) and (c) positive (intensity score 2, percentage score 3) and (d) positive (intensity score 3, percentage score 3).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4863092&req=5

fig1: NMNAT2 immunohistochemical staining for colorectal cancer peritumoral tissues (×200). (a) Negative (intensity score 0, percentage score 0) and (b) negative (intensity score 1, percentage score 1) and (c) positive (intensity score 2, percentage score 3) and (d) positive (intensity score 3, percentage score 3).
Mentions: NMNAT2 protein expression was localized primarily in cytoplasm and the diffusely cytoplasmic brown-staining was observed in positive tissues as shown in Figure 1. The specificity of the anti-NMNAT2 antibody was confirmed by western blot measuring different pairs of tumor-peritumoral tissue samples of CRC (see Figure S1 in Supplementary Material available online at http://dx.doi.org/10.1155/2016/1804137). The NMNAT2 expression could be detected in 78 of 95 (82.1%) cases among CRC tissues compared to 34 of 95 (35.7%) for adjacent normal tissues, which suggested NMNAT2 was significantly upregulated in CRC (Table 1). On the other hand, p53 protein was mainly presented in nuclear of cancer cells (Figure 2). Our results showed that the protein expression of p53 in CRC tissues (60 of 95, 63.1%) was significantly more than adjacent normal tissues (16 of 95, 16.8%) (P < 0.01).

Bottom Line: Significant positive correlation was found between the expression of NMNAT2 and the expression of p53.However, NMNAT2 expression was not a statistically significant prognostic factor for overall survival.In conclusion, our results indicated that NMNAT2 might participate in tumorigenesis of CRC in a p53-dependent manner and NMNAT2 expression might be a potential therapeutic target for CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

ABSTRACT
Colorectal cancer (CRC) is one of the most common cancers all over the world. It is essential to search for more effective diagnostic and therapeutic methods for CRC. Abnormal nicotinamide adenine dinucleotide (NAD) metabolism has been considered as a characteristic of cancer cells. In this study, nicotinamide mononucleotide adenylyl transferases (NMNATs) as well as p53-mediated cancer signaling pathways were investigated in patients with colorectal cancer. The CRC tissues and adjacent normal tissues were obtained from 95 untreated colorectal cancer patients and were stained for expression of nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) and p53. The survival rate was analyzed by the Kaplan-Meier method and the log-rank test. The multivariate Cox proportional hazard regression analysis was conducted as well. Our data demonstrated that expression of NMNAT2 and p53 was significantly higher in CRC tissues, while NMNAT2 expression is in correlation with the invasive depth of tumors and TNM stage. Significant positive correlation was found between the expression of NMNAT2 and the expression of p53. However, NMNAT2 expression was not a statistically significant prognostic factor for overall survival. In conclusion, our results indicated that NMNAT2 might participate in tumorigenesis of CRC in a p53-dependent manner and NMNAT2 expression might be a potential therapeutic target for CRC.

No MeSH data available.


Related in: MedlinePlus