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MicroRNA Profiling in Patients with Upper Tract Urothelial Carcinoma Associated with Balkan Endemic Nephropathy.

Popovska-Jankovic K, Noveski P, Jankovic-Velickovic L, Stojnev S, Cukuranovic R, Stefanovic V, Toncheva D, Staneva R, Polenakovic M, Plaseska-Karanfilska D - Biomed Res Int (2016)

Bottom Line: There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease.Aberrantly expressed miRNAs have been shown to be associated with many types of cancers.We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

View Article: PubMed Central - PubMed

Affiliation: Research Centre for Genetic Engineering and Biotechnology "Georgi D. Efremov", Macedonian Academy of Sciences and Arts, 1000 Skopje, Macedonia.

ABSTRACT
Balkan endemic nephropathy (BEN) is a disease that affects people that live in the alluvial plains along the tributaries of the Danube River in the Balkan region. BEN is a chronic tubulointerstitial disease with a slow progression to terminal renal failure and has strong association with upper tract urothelial carcinoma (UTUC). There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease. Aberrantly expressed miRNAs have been shown to be associated with many types of cancers. A number of studies have investigated the expression of microRNAs in urothelial carcinoma, mainly on urothelial bladder cancer, and only a few have included patients with UTUC. Here we present the first study of microRNA profiling in UTUC tissues from patients with BEN (BEN-UTUC) and patients with UTUC from nonendemic Balkan regions (non-BEN-UTUC) in comparison to normal kidney tissues. We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

No MeSH data available.


Related in: MedlinePlus

Comparison of qPCR and microarray data in non-BEN-UTUC samples.
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fig3: Comparison of qPCR and microarray data in non-BEN-UTUC samples.

Mentions: With the exception of hsa-miR-99b-3p, the qPCR were in agreement with the microarray results (Figures 2 and 3). In particular, hsa-miR-205-5p was upregulated in both BEN-UTUC and non-BEN-UTUC, and hsa-miR-210-3p and hsa-miR-224-3p were overexpressed with a log FC >2 in non-BEN-UTUC, while hsa-miR-373-5p was overexpressed and hsa-miR-30a-5p was underexpressed in BEN-UTUC samples.


MicroRNA Profiling in Patients with Upper Tract Urothelial Carcinoma Associated with Balkan Endemic Nephropathy.

Popovska-Jankovic K, Noveski P, Jankovic-Velickovic L, Stojnev S, Cukuranovic R, Stefanovic V, Toncheva D, Staneva R, Polenakovic M, Plaseska-Karanfilska D - Biomed Res Int (2016)

Comparison of qPCR and microarray data in non-BEN-UTUC samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4863087&req=5

fig3: Comparison of qPCR and microarray data in non-BEN-UTUC samples.
Mentions: With the exception of hsa-miR-99b-3p, the qPCR were in agreement with the microarray results (Figures 2 and 3). In particular, hsa-miR-205-5p was upregulated in both BEN-UTUC and non-BEN-UTUC, and hsa-miR-210-3p and hsa-miR-224-3p were overexpressed with a log FC >2 in non-BEN-UTUC, while hsa-miR-373-5p was overexpressed and hsa-miR-30a-5p was underexpressed in BEN-UTUC samples.

Bottom Line: There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease.Aberrantly expressed miRNAs have been shown to be associated with many types of cancers.We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

View Article: PubMed Central - PubMed

Affiliation: Research Centre for Genetic Engineering and Biotechnology "Georgi D. Efremov", Macedonian Academy of Sciences and Arts, 1000 Skopje, Macedonia.

ABSTRACT
Balkan endemic nephropathy (BEN) is a disease that affects people that live in the alluvial plains along the tributaries of the Danube River in the Balkan region. BEN is a chronic tubulointerstitial disease with a slow progression to terminal renal failure and has strong association with upper tract urothelial carcinoma (UTUC). There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease. Aberrantly expressed miRNAs have been shown to be associated with many types of cancers. A number of studies have investigated the expression of microRNAs in urothelial carcinoma, mainly on urothelial bladder cancer, and only a few have included patients with UTUC. Here we present the first study of microRNA profiling in UTUC tissues from patients with BEN (BEN-UTUC) and patients with UTUC from nonendemic Balkan regions (non-BEN-UTUC) in comparison to normal kidney tissues. We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

No MeSH data available.


Related in: MedlinePlus