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MicroRNA Profiling in Patients with Upper Tract Urothelial Carcinoma Associated with Balkan Endemic Nephropathy.

Popovska-Jankovic K, Noveski P, Jankovic-Velickovic L, Stojnev S, Cukuranovic R, Stefanovic V, Toncheva D, Staneva R, Polenakovic M, Plaseska-Karanfilska D - Biomed Res Int (2016)

Bottom Line: There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease.Aberrantly expressed miRNAs have been shown to be associated with many types of cancers.We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

View Article: PubMed Central - PubMed

Affiliation: Research Centre for Genetic Engineering and Biotechnology "Georgi D. Efremov", Macedonian Academy of Sciences and Arts, 1000 Skopje, Macedonia.

ABSTRACT
Balkan endemic nephropathy (BEN) is a disease that affects people that live in the alluvial plains along the tributaries of the Danube River in the Balkan region. BEN is a chronic tubulointerstitial disease with a slow progression to terminal renal failure and has strong association with upper tract urothelial carcinoma (UTUC). There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease. Aberrantly expressed miRNAs have been shown to be associated with many types of cancers. A number of studies have investigated the expression of microRNAs in urothelial carcinoma, mainly on urothelial bladder cancer, and only a few have included patients with UTUC. Here we present the first study of microRNA profiling in UTUC tissues from patients with BEN (BEN-UTUC) and patients with UTUC from nonendemic Balkan regions (non-BEN-UTUC) in comparison to normal kidney tissues. We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

No MeSH data available.


Related in: MedlinePlus

Venn diagrams of differentially expressed microRNAs in BEN-UTUC samples (a) and non-BEN-UTUC samples (b), both in comparison to normal kidney samples and determined by GeneSpring (GS) and R Bioconductor (RB) statistical programs.
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fig1: Venn diagrams of differentially expressed microRNAs in BEN-UTUC samples (a) and non-BEN-UTUC samples (b), both in comparison to normal kidney samples and determined by GeneSpring (GS) and R Bioconductor (RB) statistical programs.

Mentions: Preliminary qPCR analysis with RNU 44 control microRNA revealed that 15 RNA samples were with satisfactory quality for microRNA microarray analysis, including 7 samples with BEN-UTUC, 4 non-BEN-UTUC samples, and 4 nontumor samples. Statistical analysis using R Bioconductor and GeneSpring v12.5 software revealed a number of differentially expressed miRNAs (P < 0.05) and at least two log fold changes when BEN-UTUC and non-BEN-UTUC were compared to the normal kidney samples (Figure 1). The analysis using GeneSpring software showed a total of 31 differentially expressed microRNAs, while the R Bioconductor software showed 16 differentially expressed microRNAs in BEN-UTUC samples. Ten microRNAs were differentially expressed with both statistical programs (Figure 1(a)). The comparison between non-BEN-UTUC and normal kidney tissues showed 24 and 20 differentially expressed microRNAs with GeneSpring and R Bioconductor, respectively, of which 15 were detected with both types of software (Figure 1(b)).


MicroRNA Profiling in Patients with Upper Tract Urothelial Carcinoma Associated with Balkan Endemic Nephropathy.

Popovska-Jankovic K, Noveski P, Jankovic-Velickovic L, Stojnev S, Cukuranovic R, Stefanovic V, Toncheva D, Staneva R, Polenakovic M, Plaseska-Karanfilska D - Biomed Res Int (2016)

Venn diagrams of differentially expressed microRNAs in BEN-UTUC samples (a) and non-BEN-UTUC samples (b), both in comparison to normal kidney samples and determined by GeneSpring (GS) and R Bioconductor (RB) statistical programs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4863087&req=5

fig1: Venn diagrams of differentially expressed microRNAs in BEN-UTUC samples (a) and non-BEN-UTUC samples (b), both in comparison to normal kidney samples and determined by GeneSpring (GS) and R Bioconductor (RB) statistical programs.
Mentions: Preliminary qPCR analysis with RNU 44 control microRNA revealed that 15 RNA samples were with satisfactory quality for microRNA microarray analysis, including 7 samples with BEN-UTUC, 4 non-BEN-UTUC samples, and 4 nontumor samples. Statistical analysis using R Bioconductor and GeneSpring v12.5 software revealed a number of differentially expressed miRNAs (P < 0.05) and at least two log fold changes when BEN-UTUC and non-BEN-UTUC were compared to the normal kidney samples (Figure 1). The analysis using GeneSpring software showed a total of 31 differentially expressed microRNAs, while the R Bioconductor software showed 16 differentially expressed microRNAs in BEN-UTUC samples. Ten microRNAs were differentially expressed with both statistical programs (Figure 1(a)). The comparison between non-BEN-UTUC and normal kidney tissues showed 24 and 20 differentially expressed microRNAs with GeneSpring and R Bioconductor, respectively, of which 15 were detected with both types of software (Figure 1(b)).

Bottom Line: There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease.Aberrantly expressed miRNAs have been shown to be associated with many types of cancers.We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

View Article: PubMed Central - PubMed

Affiliation: Research Centre for Genetic Engineering and Biotechnology "Georgi D. Efremov", Macedonian Academy of Sciences and Arts, 1000 Skopje, Macedonia.

ABSTRACT
Balkan endemic nephropathy (BEN) is a disease that affects people that live in the alluvial plains along the tributaries of the Danube River in the Balkan region. BEN is a chronic tubulointerstitial disease with a slow progression to terminal renal failure and has strong association with upper tract urothelial carcinoma (UTUC). There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease. Aberrantly expressed miRNAs have been shown to be associated with many types of cancers. A number of studies have investigated the expression of microRNAs in urothelial carcinoma, mainly on urothelial bladder cancer, and only a few have included patients with UTUC. Here we present the first study of microRNA profiling in UTUC tissues from patients with BEN (BEN-UTUC) and patients with UTUC from nonendemic Balkan regions (non-BEN-UTUC) in comparison to normal kidney tissues. We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups.

No MeSH data available.


Related in: MedlinePlus