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Spike Code Flow in Cultured Neuronal Networks.

Tamura S, Nishitani Y, Hosokawa C, Miyoshi T, Sawai H, Kamimura T, Yagi Y, Mizuno-Matsumoto Y, Chen YW - Comput Intell Neurosci (2016)

Bottom Line: Furthermore, if the spike trains were shuffled in interval orders or in electrodes, they became significantly small.Thus, the analysis suggested that local codes of approximately constant shape propagated and conveyed information across the network.Hence, the codes can serve as visible and trackable marks of propagating spike waves as well as evaluating information flow in the neuronal network.

View Article: PubMed Central - PubMed

Affiliation: NBL Technovator Co., Ltd., 631 Shindachimakino, Sennan 590-0522, Japan.

ABSTRACT
We observed spike trains produced by one-shot electrical stimulation with 8 × 8 multielectrodes in cultured neuronal networks. Each electrode accepted spikes from several neurons. We extracted the short codes from spike trains and obtained a code spectrum with a nominal time accuracy of 1%. We then constructed code flow maps as movies of the electrode array to observe the code flow of "1101" and "1011," which are typical pseudorandom sequence such as that we often encountered in a literature and our experiments. They seemed to flow from one electrode to the neighboring one and maintained their shape to some extent. To quantify the flow, we calculated the "maximum cross-correlations" among neighboring electrodes, to find the direction of maximum flow of the codes with lengths less than 8. Normalized maximum cross-correlations were almost constant irrespective of code. Furthermore, if the spike trains were shuffled in interval orders or in electrodes, they became significantly small. Thus, the analysis suggested that local codes of approximately constant shape propagated and conveyed information across the network. Hence, the codes can serve as visible and trackable marks of propagating spike waves as well as evaluating information flow in the neuronal network.

No MeSH data available.


Related in: MedlinePlus

(Upper) Micrograph of cultured hippocampal neurons in a microelectrode array. Black rectangles indicate electrodes. (Lower) Illustration of a vertical section. Each electrode catches spikes from several neurons. We can observe spike trains containing code such as “1011.” Each bit (“1” or “0”) is considered from different neuron for short time length (short bit width) code, since it takes more time for the same neuron to fire twice than the refractory period.
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fig1: (Upper) Micrograph of cultured hippocampal neurons in a microelectrode array. Black rectangles indicate electrodes. (Lower) Illustration of a vertical section. Each electrode catches spikes from several neurons. We can observe spike trains containing code such as “1011.” Each bit (“1” or “0”) is considered from different neuron for short time length (short bit width) code, since it takes more time for the same neuron to fire twice than the refractory period.

Mentions: The cell cultures of hippocampal neurons were dissected from 18-day-old Wistar rat embryos and implanted on microelectrode array dishes (MED-P515A, Alpha MED Scientific Inc., Kadoma, Osaka, Japan) with 8 × 8 planar microelectrodes as shown in Figure 1 [14]. In the present study, the same raster plot data were used as [15], which are composed of 0.1 ms bins.


Spike Code Flow in Cultured Neuronal Networks.

Tamura S, Nishitani Y, Hosokawa C, Miyoshi T, Sawai H, Kamimura T, Yagi Y, Mizuno-Matsumoto Y, Chen YW - Comput Intell Neurosci (2016)

(Upper) Micrograph of cultured hippocampal neurons in a microelectrode array. Black rectangles indicate electrodes. (Lower) Illustration of a vertical section. Each electrode catches spikes from several neurons. We can observe spike trains containing code such as “1011.” Each bit (“1” or “0”) is considered from different neuron for short time length (short bit width) code, since it takes more time for the same neuron to fire twice than the refractory period.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4863084&req=5

fig1: (Upper) Micrograph of cultured hippocampal neurons in a microelectrode array. Black rectangles indicate electrodes. (Lower) Illustration of a vertical section. Each electrode catches spikes from several neurons. We can observe spike trains containing code such as “1011.” Each bit (“1” or “0”) is considered from different neuron for short time length (short bit width) code, since it takes more time for the same neuron to fire twice than the refractory period.
Mentions: The cell cultures of hippocampal neurons were dissected from 18-day-old Wistar rat embryos and implanted on microelectrode array dishes (MED-P515A, Alpha MED Scientific Inc., Kadoma, Osaka, Japan) with 8 × 8 planar microelectrodes as shown in Figure 1 [14]. In the present study, the same raster plot data were used as [15], which are composed of 0.1 ms bins.

Bottom Line: Furthermore, if the spike trains were shuffled in interval orders or in electrodes, they became significantly small.Thus, the analysis suggested that local codes of approximately constant shape propagated and conveyed information across the network.Hence, the codes can serve as visible and trackable marks of propagating spike waves as well as evaluating information flow in the neuronal network.

View Article: PubMed Central - PubMed

Affiliation: NBL Technovator Co., Ltd., 631 Shindachimakino, Sennan 590-0522, Japan.

ABSTRACT
We observed spike trains produced by one-shot electrical stimulation with 8 × 8 multielectrodes in cultured neuronal networks. Each electrode accepted spikes from several neurons. We extracted the short codes from spike trains and obtained a code spectrum with a nominal time accuracy of 1%. We then constructed code flow maps as movies of the electrode array to observe the code flow of "1101" and "1011," which are typical pseudorandom sequence such as that we often encountered in a literature and our experiments. They seemed to flow from one electrode to the neighboring one and maintained their shape to some extent. To quantify the flow, we calculated the "maximum cross-correlations" among neighboring electrodes, to find the direction of maximum flow of the codes with lengths less than 8. Normalized maximum cross-correlations were almost constant irrespective of code. Furthermore, if the spike trains were shuffled in interval orders or in electrodes, they became significantly small. Thus, the analysis suggested that local codes of approximately constant shape propagated and conveyed information across the network. Hence, the codes can serve as visible and trackable marks of propagating spike waves as well as evaluating information flow in the neuronal network.

No MeSH data available.


Related in: MedlinePlus