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Neural clocks and Neuropeptide F/Y regulate circadian gene expression in a peripheral metabolic tissue.

Erion R, King AN, Wu G, Hogenesch JB, Sehgal A - Elife (2016)

Bottom Line: Interestingly, rhythmic expression of the cytochrome P450 transcripts, sex-specific enzyme 1 (sxe1) and Cyp6a21, which cycle in the fat body independently of the local clock, depends upon clocks in neurons expressing neuropeptide F (NPF).NPF signaling itself is required to drive cycling of sxe1 and Cyp6a21 in the fat body, and its mammalian ortholog, Npy, functions similarly to regulate cycling of cytochrome P450 genes in the mouse liver.These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms.

View Article: PubMed Central - PubMed

Affiliation: Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia, United States.

ABSTRACT
Metabolic homeostasis requires coordination between circadian clocks in different tissues. Also, systemic signals appear to be required for some transcriptional rhythms in the mammalian liver and the Drosophila fat body. Here we show that free-running oscillations of the fat body clock require clock function in the PDF-positive cells of the fly brain. Interestingly, rhythmic expression of the cytochrome P450 transcripts, sex-specific enzyme 1 (sxe1) and Cyp6a21, which cycle in the fat body independently of the local clock, depends upon clocks in neurons expressing neuropeptide F (NPF). NPF signaling itself is required to drive cycling of sxe1 and Cyp6a21 in the fat body, and its mammalian ortholog, Npy, functions similarly to regulate cycling of cytochrome P450 genes in the mouse liver. These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms.

No MeSH data available.


Restricted feeding between ZT9 and ZT15 alters the rhythmic expression of sxe1 and clock gene per in the Drosophila fat body.DOI:http://dx.doi.org/10.7554/eLife.13552.021
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fig7: Restricted feeding between ZT9 and ZT15 alters the rhythmic expression of sxe1 and clock gene per in the Drosophila fat body.DOI:http://dx.doi.org/10.7554/eLife.13552.021

Mentions: We include a time-restricted feeding (TRF) experiment (Author response image 1), and list the reasons we believe that although feeding can affect cycling of sxe1, it is not the only, and most likely not even the major, contributor to rhythms of genes driven by NPF/NPY:10.7554/eLife.13552.021Author response image 1.Restricted feeding between ZT9 and ZT15 alters the rhythmic expression of sxe1 and clock gene per in the Drosophila fat body.


Neural clocks and Neuropeptide F/Y regulate circadian gene expression in a peripheral metabolic tissue.

Erion R, King AN, Wu G, Hogenesch JB, Sehgal A - Elife (2016)

Restricted feeding between ZT9 and ZT15 alters the rhythmic expression of sxe1 and clock gene per in the Drosophila fat body.DOI:http://dx.doi.org/10.7554/eLife.13552.021
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4862751&req=5

fig7: Restricted feeding between ZT9 and ZT15 alters the rhythmic expression of sxe1 and clock gene per in the Drosophila fat body.DOI:http://dx.doi.org/10.7554/eLife.13552.021
Mentions: We include a time-restricted feeding (TRF) experiment (Author response image 1), and list the reasons we believe that although feeding can affect cycling of sxe1, it is not the only, and most likely not even the major, contributor to rhythms of genes driven by NPF/NPY:10.7554/eLife.13552.021Author response image 1.Restricted feeding between ZT9 and ZT15 alters the rhythmic expression of sxe1 and clock gene per in the Drosophila fat body.

Bottom Line: Interestingly, rhythmic expression of the cytochrome P450 transcripts, sex-specific enzyme 1 (sxe1) and Cyp6a21, which cycle in the fat body independently of the local clock, depends upon clocks in neurons expressing neuropeptide F (NPF).NPF signaling itself is required to drive cycling of sxe1 and Cyp6a21 in the fat body, and its mammalian ortholog, Npy, functions similarly to regulate cycling of cytochrome P450 genes in the mouse liver.These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms.

View Article: PubMed Central - PubMed

Affiliation: Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia, United States.

ABSTRACT
Metabolic homeostasis requires coordination between circadian clocks in different tissues. Also, systemic signals appear to be required for some transcriptional rhythms in the mammalian liver and the Drosophila fat body. Here we show that free-running oscillations of the fat body clock require clock function in the PDF-positive cells of the fly brain. Interestingly, rhythmic expression of the cytochrome P450 transcripts, sex-specific enzyme 1 (sxe1) and Cyp6a21, which cycle in the fat body independently of the local clock, depends upon clocks in neurons expressing neuropeptide F (NPF). NPF signaling itself is required to drive cycling of sxe1 and Cyp6a21 in the fat body, and its mammalian ortholog, Npy, functions similarly to regulate cycling of cytochrome P450 genes in the mouse liver. These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms.

No MeSH data available.