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Involvement of NADH Oxidase in Competition and Endocarditis Virulence in Streptococcus sanguinis.

Ge X, Yu Y, Zhang M, Chen L, Chen W, Elrami F, Kong F, Kitten T, Xu P - Infect. Immun. (2016)

Bottom Line: An S. sanguinis nox mutant was found to fail to inhibit the growth of Streptococcus mutans under microaerobic conditions.The nox mutant exhibited decreased levels of extracellular H2O2; however, the intracellular level of H2O2 in the mutant was increased.The nox mutant also was shown to be more sensitive to blood killing, oxidative and acid stresses, and reduced growth in serum.

View Article: PubMed Central - PubMed

Affiliation: Philips Institute for Oral Health Research, Virginia Commonwealth University, Richmond, Virginia, USA.

No MeSH data available.


Related in: MedlinePlus

Sensitivity to environmental stresses in the nox mutant. Reduction in bacterial survival upon exposure to exogenous H2O2 (A) and acid (B) in the nox mutant. Δnox, the nox mutant; Δnox_compl, the complemented strain of the nox mutant. An asterisk indicates a significant difference at a P value of <0.05 compared to SK36. **, P < 0.01. Data were obtained at least in biological triplicate.
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Figure 7: Sensitivity to environmental stresses in the nox mutant. Reduction in bacterial survival upon exposure to exogenous H2O2 (A) and acid (B) in the nox mutant. Δnox, the nox mutant; Δnox_compl, the complemented strain of the nox mutant. An asterisk indicates a significant difference at a P value of <0.05 compared to SK36. **, P < 0.01. Data were obtained at least in biological triplicate.

Mentions: Neutrophil oxidative burst and acidification of phagosomes have been implicated in the bactericidal function of phagocytes (30); therefore, the sensitivity of the nox mutant to exogenous H2O2 and acid was examined. With H2O2 treatment, the survival of the nox mutant was markedly reduced after 1 h compared to that of the wild type. Complementation of the nox mutant restored survival to the same level as that of the wild type (Fig. 7A). Upon acid treatment, survival of the nox mutant also exhibited a significant decrease compared to that of the wild type (Fig. 7B). These data indicated that the nox mutant was more sensitive to H2O2 and acid stresses than the wild type.


Involvement of NADH Oxidase in Competition and Endocarditis Virulence in Streptococcus sanguinis.

Ge X, Yu Y, Zhang M, Chen L, Chen W, Elrami F, Kong F, Kitten T, Xu P - Infect. Immun. (2016)

Sensitivity to environmental stresses in the nox mutant. Reduction in bacterial survival upon exposure to exogenous H2O2 (A) and acid (B) in the nox mutant. Δnox, the nox mutant; Δnox_compl, the complemented strain of the nox mutant. An asterisk indicates a significant difference at a P value of <0.05 compared to SK36. **, P < 0.01. Data were obtained at least in biological triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4862721&req=5

Figure 7: Sensitivity to environmental stresses in the nox mutant. Reduction in bacterial survival upon exposure to exogenous H2O2 (A) and acid (B) in the nox mutant. Δnox, the nox mutant; Δnox_compl, the complemented strain of the nox mutant. An asterisk indicates a significant difference at a P value of <0.05 compared to SK36. **, P < 0.01. Data were obtained at least in biological triplicate.
Mentions: Neutrophil oxidative burst and acidification of phagosomes have been implicated in the bactericidal function of phagocytes (30); therefore, the sensitivity of the nox mutant to exogenous H2O2 and acid was examined. With H2O2 treatment, the survival of the nox mutant was markedly reduced after 1 h compared to that of the wild type. Complementation of the nox mutant restored survival to the same level as that of the wild type (Fig. 7A). Upon acid treatment, survival of the nox mutant also exhibited a significant decrease compared to that of the wild type (Fig. 7B). These data indicated that the nox mutant was more sensitive to H2O2 and acid stresses than the wild type.

Bottom Line: An S. sanguinis nox mutant was found to fail to inhibit the growth of Streptococcus mutans under microaerobic conditions.The nox mutant exhibited decreased levels of extracellular H2O2; however, the intracellular level of H2O2 in the mutant was increased.The nox mutant also was shown to be more sensitive to blood killing, oxidative and acid stresses, and reduced growth in serum.

View Article: PubMed Central - PubMed

Affiliation: Philips Institute for Oral Health Research, Virginia Commonwealth University, Richmond, Virginia, USA.

No MeSH data available.


Related in: MedlinePlus