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Chronic Physical Stress Does Not Interact with Epstein-Barr Virus (EBV)-Encoded Dutpase to Alter the Sickness Response.

Aubrecht TG, Weil ZM, Abi Salloum B, Ariza ME, Williams M, Reader B, Glaser R, Sheridan J, Nelson RJ - J Behav Brain Sci (2015)

Bottom Line: Most adult humans have been infected with Epstein-Barr virus (EBV), which is thought to contribute to the development of chronic fatigue syndrome.In this study, we seek to determine the interaction of chronic physical (swim) stress and EBV-encoded dUTPase injection.The physical swimming stress does not alter the sickness response.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departments of Neuroscience, The Ohio State University, Columbus, OH, USA.

ABSTRACT

Most adult humans have been infected with Epstein-Barr virus (EBV), which is thought to contribute to the development of chronic fatigue syndrome. Stress is known to influence the immune system and can exacerbate the sickness response. Although a role for psychological stress in the sickness response, particularly in combination with EBV-encoded deoxyuridine triphosphate nucleotidohydrolase (dUTPase) has been established, and the role of physical stressors in these interactions remains unspecified. In this study, we seek to determine the interaction of chronic physical (swim) stress and EBV-encoded dUTPase injection. We hypothesize that a chronic physical stressor will exacerbate the sickness response following EBV-encoded dUTPase injection. To test this hypothesis mice receive daily injections of EBV-encoded dUTPase or vehicle and are subjected to 15 min of swim stress each day for 14 days or left unmanipulated. On the final evening of injections mice undergo behavioral testing. EBV-encoded dUTPase injection alone produces some sickness behaviors. The physical swimming stress does not alter the sickness response.

No MeSH data available.


Related in: MedlinePlus

On day one, body temperature (±SEM) was reduced in mice that swam compared to mice that did not swim (a), significance marked on average body temperature graph for day 1 (d). Body temperature averaged from days 1 - 7 was not altered by swimming of EBV dUTPase injections (b). EBV dUTPase injections decreased body temperature compared to saline injections and swimming decreased body temperature compared to mice that did not swim averaged over all 14 days ((c), (e)). Each graph represents average body temperature (±SEM), across multiple days (for (b) and (c)), in the hours following injection. Asterisks (*) indicates significant main effect differences in repeated measures ANOVA, p < 0.05.
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Figure 1: On day one, body temperature (±SEM) was reduced in mice that swam compared to mice that did not swim (a), significance marked on average body temperature graph for day 1 (d). Body temperature averaged from days 1 - 7 was not altered by swimming of EBV dUTPase injections (b). EBV dUTPase injections decreased body temperature compared to saline injections and swimming decreased body temperature compared to mice that did not swim averaged over all 14 days ((c), (e)). Each graph represents average body temperature (±SEM), across multiple days (for (b) and (c)), in the hours following injection. Asterisks (*) indicates significant main effect differences in repeated measures ANOVA, p < 0.05.

Mentions: Body temperature on day one increased during the active phase (lights off) compared to inactive phase (lights on) (F42,924 = 8.68, p < 0.01, Figure 1(a)). On day one, body temperature was reduced (F42,924 = 2.11, p < 0.01, Figure 1(a), Figure 1(d)) in mice that swam compared to mice that did not swim. Body temperature was not affected by EBV-encoded dUTPase (p > 0.05, Figure 1(a)) on day 1. Body temperature was not altered by EBV-encoded dUTPase or swimming averaged over the first seven days (p > 0.05, Figure 1(b)). Body temperature increased during the active phase (lights off) compared to inactive phase (lights on) (F42,924 = 49.6, p < 0.01, Figure 1(b)) averaged over the first 7 days. There was no interaction of EBV-encoded dUTPase and swimming on body temperature on any of the 14 days (p > 0.05, Figure 1(c)). EBV-encoded dUTPase injections decreased body temperature compared to saline injections (F42,1344 = 1.49, p < 0.05, Figure 1(c), Figure 1(e)) averaged over all 14 days. Swimming decreased body temperature compared to mice that did not swim (F42,1344 = 1.49, p < 0.05, Figure 1(c), Figure 1(e)) averaged over all 14 days. Body temperature increased during the active phase (lights off) compared to inactive phase (lights on) (F42,924 = 95.2, p < 0.01, Figure 1(c)) averaged over all 14 days.


Chronic Physical Stress Does Not Interact with Epstein-Barr Virus (EBV)-Encoded Dutpase to Alter the Sickness Response.

Aubrecht TG, Weil ZM, Abi Salloum B, Ariza ME, Williams M, Reader B, Glaser R, Sheridan J, Nelson RJ - J Behav Brain Sci (2015)

On day one, body temperature (±SEM) was reduced in mice that swam compared to mice that did not swim (a), significance marked on average body temperature graph for day 1 (d). Body temperature averaged from days 1 - 7 was not altered by swimming of EBV dUTPase injections (b). EBV dUTPase injections decreased body temperature compared to saline injections and swimming decreased body temperature compared to mice that did not swim averaged over all 14 days ((c), (e)). Each graph represents average body temperature (±SEM), across multiple days (for (b) and (c)), in the hours following injection. Asterisks (*) indicates significant main effect differences in repeated measures ANOVA, p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4862656&req=5

Figure 1: On day one, body temperature (±SEM) was reduced in mice that swam compared to mice that did not swim (a), significance marked on average body temperature graph for day 1 (d). Body temperature averaged from days 1 - 7 was not altered by swimming of EBV dUTPase injections (b). EBV dUTPase injections decreased body temperature compared to saline injections and swimming decreased body temperature compared to mice that did not swim averaged over all 14 days ((c), (e)). Each graph represents average body temperature (±SEM), across multiple days (for (b) and (c)), in the hours following injection. Asterisks (*) indicates significant main effect differences in repeated measures ANOVA, p < 0.05.
Mentions: Body temperature on day one increased during the active phase (lights off) compared to inactive phase (lights on) (F42,924 = 8.68, p < 0.01, Figure 1(a)). On day one, body temperature was reduced (F42,924 = 2.11, p < 0.01, Figure 1(a), Figure 1(d)) in mice that swam compared to mice that did not swim. Body temperature was not affected by EBV-encoded dUTPase (p > 0.05, Figure 1(a)) on day 1. Body temperature was not altered by EBV-encoded dUTPase or swimming averaged over the first seven days (p > 0.05, Figure 1(b)). Body temperature increased during the active phase (lights off) compared to inactive phase (lights on) (F42,924 = 49.6, p < 0.01, Figure 1(b)) averaged over the first 7 days. There was no interaction of EBV-encoded dUTPase and swimming on body temperature on any of the 14 days (p > 0.05, Figure 1(c)). EBV-encoded dUTPase injections decreased body temperature compared to saline injections (F42,1344 = 1.49, p < 0.05, Figure 1(c), Figure 1(e)) averaged over all 14 days. Swimming decreased body temperature compared to mice that did not swim (F42,1344 = 1.49, p < 0.05, Figure 1(c), Figure 1(e)) averaged over all 14 days. Body temperature increased during the active phase (lights off) compared to inactive phase (lights on) (F42,924 = 95.2, p < 0.01, Figure 1(c)) averaged over all 14 days.

Bottom Line: Most adult humans have been infected with Epstein-Barr virus (EBV), which is thought to contribute to the development of chronic fatigue syndrome.In this study, we seek to determine the interaction of chronic physical (swim) stress and EBV-encoded dUTPase injection.The physical swimming stress does not alter the sickness response.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departments of Neuroscience, The Ohio State University, Columbus, OH, USA.

ABSTRACT

Most adult humans have been infected with Epstein-Barr virus (EBV), which is thought to contribute to the development of chronic fatigue syndrome. Stress is known to influence the immune system and can exacerbate the sickness response. Although a role for psychological stress in the sickness response, particularly in combination with EBV-encoded deoxyuridine triphosphate nucleotidohydrolase (dUTPase) has been established, and the role of physical stressors in these interactions remains unspecified. In this study, we seek to determine the interaction of chronic physical (swim) stress and EBV-encoded dUTPase injection. We hypothesize that a chronic physical stressor will exacerbate the sickness response following EBV-encoded dUTPase injection. To test this hypothesis mice receive daily injections of EBV-encoded dUTPase or vehicle and are subjected to 15 min of swim stress each day for 14 days or left unmanipulated. On the final evening of injections mice undergo behavioral testing. EBV-encoded dUTPase injection alone produces some sickness behaviors. The physical swimming stress does not alter the sickness response.

No MeSH data available.


Related in: MedlinePlus