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Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

Xue B, He D, Gao S, Wang D, Yokoyama K, Wang L - Int J Nanomedicine (2016)

Bottom Line: X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm.Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis.Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, People's Republic of China.

ABSTRACT
The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs. X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm. Numerous factors could potentially affect the process of biosynthesis, and the main factors are discussed here. Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis. Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

No MeSH data available.


Related in: MedlinePlus

TEM image and size distribution of the AgNPs.Notes: (A) Representative images of AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum (scale bar =100 nm) and (B, C) size distribution of the AgNPs from Malvern Zetasizer Nano ZS analysis after being biosynthesized 10 hours and 2 months, respectively.Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.
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f5-ijn-11-1899: TEM image and size distribution of the AgNPs.Notes: (A) Representative images of AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum (scale bar =100 nm) and (B, C) size distribution of the AgNPs from Malvern Zetasizer Nano ZS analysis after being biosynthesized 10 hours and 2 months, respectively.Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.

Mentions: A TEM image of the AgNPs is shown in Figure 5A, showing that they are spherical in shape. The average diameter was 15.5±2.5 nm, with a highly uniform and narrow distribution of diameters (data not shown). Similar observations have previously been reported by Jaidev and Narasimha.31 A Malvern Zetasizer Nano ZS was used to analyze the diameters more precisely. Figure 5B shows an average diameter of 20.56 nm, with 5.8% of the particles being <1.92 nm and 94.2% being <36.10 nm in diameter, which was similar to the TEM data. The AgNPs were mono-dispersed with a low polydispersity index of 0.27. The size measured by TEM micrographs was slightly smaller than that measured by Malvern Zetasizer Nano ZS. This may have been caused by the presence of attached surface proteins, carbohydrates, and other cellular materials, which would have been measured by the Zetasizer but would not have been retained in a vacuum under an electron beam. This may also explain the reason for the stable presence of biosynthesized nanoparticles.


Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

Xue B, He D, Gao S, Wang D, Yokoyama K, Wang L - Int J Nanomedicine (2016)

TEM image and size distribution of the AgNPs.Notes: (A) Representative images of AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum (scale bar =100 nm) and (B, C) size distribution of the AgNPs from Malvern Zetasizer Nano ZS analysis after being biosynthesized 10 hours and 2 months, respectively.Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862354&req=5

f5-ijn-11-1899: TEM image and size distribution of the AgNPs.Notes: (A) Representative images of AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum (scale bar =100 nm) and (B, C) size distribution of the AgNPs from Malvern Zetasizer Nano ZS analysis after being biosynthesized 10 hours and 2 months, respectively.Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.
Mentions: A TEM image of the AgNPs is shown in Figure 5A, showing that they are spherical in shape. The average diameter was 15.5±2.5 nm, with a highly uniform and narrow distribution of diameters (data not shown). Similar observations have previously been reported by Jaidev and Narasimha.31 A Malvern Zetasizer Nano ZS was used to analyze the diameters more precisely. Figure 5B shows an average diameter of 20.56 nm, with 5.8% of the particles being <1.92 nm and 94.2% being <36.10 nm in diameter, which was similar to the TEM data. The AgNPs were mono-dispersed with a low polydispersity index of 0.27. The size measured by TEM micrographs was slightly smaller than that measured by Malvern Zetasizer Nano ZS. This may have been caused by the presence of attached surface proteins, carbohydrates, and other cellular materials, which would have been measured by the Zetasizer but would not have been retained in a vacuum under an electron beam. This may also explain the reason for the stable presence of biosynthesized nanoparticles.

Bottom Line: X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm.Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis.Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, People's Republic of China.

ABSTRACT
The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs. X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm. Numerous factors could potentially affect the process of biosynthesis, and the main factors are discussed here. Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis. Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

No MeSH data available.


Related in: MedlinePlus