Limits...
Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

Xue B, He D, Gao S, Wang D, Yokoyama K, Wang L - Int J Nanomedicine (2016)

Bottom Line: X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm.Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis.Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, People's Republic of China.

ABSTRACT
The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs. X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm. Numerous factors could potentially affect the process of biosynthesis, and the main factors are discussed here. Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis. Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

No MeSH data available.


Related in: MedlinePlus

XRD patterns of lyophilized AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4862354&req=5

f4-ijn-11-1899: XRD patterns of lyophilized AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.

Mentions: XRD was used to identify the crystalline nature of AgNPs. The XRD patterns of the lyophilized AgNPs using Arthroderma fulvum are shown in Figure 4. Five diffraction peaks at 2θ values of 31.8°, 37.9°, 45.0°, 64.3°, and 77.7° were observed, corresponding to Bragg’s reflections of metallic AgNPs crystallized in a face-centered cubic structure with basal (111), (200), (220), (311), and (222) lattice planes, respectively.19,20 The results of the XRD pattern further corroborated the biosynthesis of AgNPs, with sharp bands of Bragg peaks corresponding to crystalline AgNPs, which may result from stabilization of the AgNPs by reducing agents in the reaction. The present results clearly illustrated that AgNPs biosynthesized by this green method are nanocrystalline in nature.30


Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

Xue B, He D, Gao S, Wang D, Yokoyama K, Wang L - Int J Nanomedicine (2016)

XRD patterns of lyophilized AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862354&req=5

f4-ijn-11-1899: XRD patterns of lyophilized AgNPs biosynthesized by the reduction of AgNO3 solution with the cell filtrate of Arthroderma fulvum.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.
Mentions: XRD was used to identify the crystalline nature of AgNPs. The XRD patterns of the lyophilized AgNPs using Arthroderma fulvum are shown in Figure 4. Five diffraction peaks at 2θ values of 31.8°, 37.9°, 45.0°, 64.3°, and 77.7° were observed, corresponding to Bragg’s reflections of metallic AgNPs crystallized in a face-centered cubic structure with basal (111), (200), (220), (311), and (222) lattice planes, respectively.19,20 The results of the XRD pattern further corroborated the biosynthesis of AgNPs, with sharp bands of Bragg peaks corresponding to crystalline AgNPs, which may result from stabilization of the AgNPs by reducing agents in the reaction. The present results clearly illustrated that AgNPs biosynthesized by this green method are nanocrystalline in nature.30

Bottom Line: X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm.Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis.Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, People's Republic of China.

ABSTRACT
The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs. X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm. Numerous factors could potentially affect the process of biosynthesis, and the main factors are discussed here. Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis. Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

No MeSH data available.


Related in: MedlinePlus