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Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

Xue B, He D, Gao S, Wang D, Yokoyama K, Wang L - Int J Nanomedicine (2016)

Bottom Line: X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm.Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis.Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, People's Republic of China.

ABSTRACT
The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs. X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm. Numerous factors could potentially affect the process of biosynthesis, and the main factors are discussed here. Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis. Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

No MeSH data available.


Related in: MedlinePlus

Morphology of Arthroderma fulvum (strain HT77).Notes: (A) Macroscopic morphology (PDA, 28°C, 9 days) and (B) microscopic morphology (×400, stained with lactophenol cotton blue).Abbreviation: PDA, potato dextrose agar.
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f1-ijn-11-1899: Morphology of Arthroderma fulvum (strain HT77).Notes: (A) Macroscopic morphology (PDA, 28°C, 9 days) and (B) microscopic morphology (×400, stained with lactophenol cotton blue).Abbreviation: PDA, potato dextrose agar.

Mentions: The HT77 strain that could rapidly biosynthesize AgNPs, was obtained through the experiment. This strain grew slowly; the colonies incubated on PDA medium at 28°C were 6.5 cm in diameter at day 9. Each colony was suede-like and released red pigment into the PDA (Figure 1A). Conidia were globose to pyriform with 15–25 μm in length, darkly pigmented, had a verrucose external surface, and became multicellular (Figure 1B). Based on these typical characteristics, the strain was identified as the genus Arthroderma. BLAST analysis of the ITS sequence of fungus returned 99% homology to the ITS sequence from Arthroderma fulvum (accession number AB193716.1). Based on the degree of homology typically seen in ITS sequence-related fungi, strain HT77 was identified as Arthroderma fulvum finally.


Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

Xue B, He D, Gao S, Wang D, Yokoyama K, Wang L - Int J Nanomedicine (2016)

Morphology of Arthroderma fulvum (strain HT77).Notes: (A) Macroscopic morphology (PDA, 28°C, 9 days) and (B) microscopic morphology (×400, stained with lactophenol cotton blue).Abbreviation: PDA, potato dextrose agar.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862354&req=5

f1-ijn-11-1899: Morphology of Arthroderma fulvum (strain HT77).Notes: (A) Macroscopic morphology (PDA, 28°C, 9 days) and (B) microscopic morphology (×400, stained with lactophenol cotton blue).Abbreviation: PDA, potato dextrose agar.
Mentions: The HT77 strain that could rapidly biosynthesize AgNPs, was obtained through the experiment. This strain grew slowly; the colonies incubated on PDA medium at 28°C were 6.5 cm in diameter at day 9. Each colony was suede-like and released red pigment into the PDA (Figure 1A). Conidia were globose to pyriform with 15–25 μm in length, darkly pigmented, had a verrucose external surface, and became multicellular (Figure 1B). Based on these typical characteristics, the strain was identified as the genus Arthroderma. BLAST analysis of the ITS sequence of fungus returned 99% homology to the ITS sequence from Arthroderma fulvum (accession number AB193716.1). Based on the degree of homology typically seen in ITS sequence-related fungi, strain HT77 was identified as Arthroderma fulvum finally.

Bottom Line: X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm.Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis.Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, People's Republic of China.

ABSTRACT
The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs. X-ray diffraction and transmission electron microscopy demonstrated that the biosynthesized AgNPs were crystalline in nature with an average diameter of 15.5±2.5 nm. Numerous factors could potentially affect the process of biosynthesis, and the main factors are discussed here. Optimization results showed that substrate concentration of 1.5 mM, alkaline pH, reaction temperature of 55°C, and reaction time of 10 hours were the optimum conditions for AgNP biosynthesis. Biosynthesized AgNPs showed considerable activity against the tested fungal strains, including Candida spp., Aspergillus spp., and Fusarium spp., especially Candida spp.

No MeSH data available.


Related in: MedlinePlus