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Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo.

He Y, Du Z, Ma S, Liu Y, Li D, Huang H, Jiang S, Cheng S, Wu W, Zhang K, Zheng X - Int J Nanomedicine (2016)

Bottom Line: AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells.The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression.AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model.

View Article: PubMed Central - PubMed

Affiliation: Allan H Conney Laboratory for Anticancer Drug Research, School of Chemical Engineering and Light Industry, Guandong University of Technology, Guangzhou, People's Republic of China.

ABSTRACT
Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention.

No MeSH data available.


Related in: MedlinePlus

Effects of AgNPs on the expression of phosphorylated stat3, bcl-2, and caspase in H1299 cells.Abbreviation: AgNPs, silver nanoparticles.
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f5-ijn-11-1879: Effects of AgNPs on the expression of phosphorylated stat3, bcl-2, and caspase in H1299 cells.Abbreviation: AgNPs, silver nanoparticles.

Mentions: The effects of AgNPs on the levels of phosphorylated bcl-2, stat3, survivin, and caspase-3 in lung cancer cells were further determined by Western blotting at the concentration of 10 μg/mL. The expressions were analyzed by optical density measurements and normalized for β-actin. As shown in Figure 5, the effects of AgNPs on H1299 cells included a decrease in bcl-2 and an increase in caspase-3 and survivin levels. No changes were observed in stat3 level.


Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo.

He Y, Du Z, Ma S, Liu Y, Li D, Huang H, Jiang S, Cheng S, Wu W, Zhang K, Zheng X - Int J Nanomedicine (2016)

Effects of AgNPs on the expression of phosphorylated stat3, bcl-2, and caspase in H1299 cells.Abbreviation: AgNPs, silver nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862350&req=5

f5-ijn-11-1879: Effects of AgNPs on the expression of phosphorylated stat3, bcl-2, and caspase in H1299 cells.Abbreviation: AgNPs, silver nanoparticles.
Mentions: The effects of AgNPs on the levels of phosphorylated bcl-2, stat3, survivin, and caspase-3 in lung cancer cells were further determined by Western blotting at the concentration of 10 μg/mL. The expressions were analyzed by optical density measurements and normalized for β-actin. As shown in Figure 5, the effects of AgNPs on H1299 cells included a decrease in bcl-2 and an increase in caspase-3 and survivin levels. No changes were observed in stat3 level.

Bottom Line: AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells.The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression.AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model.

View Article: PubMed Central - PubMed

Affiliation: Allan H Conney Laboratory for Anticancer Drug Research, School of Chemical Engineering and Light Industry, Guandong University of Technology, Guangzhou, People's Republic of China.

ABSTRACT
Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention.

No MeSH data available.


Related in: MedlinePlus