Limits...
Nephroprotective effect of estrogen and progesterone combination on cisplatin-induced nephrotoxicity in ovariectomized female rats.

Ghasemi M, Nematbakhsh M, Pezeshki Z, Soltani N, Moeini M, Talebi A - Indian J Nephrol (2016 May-Jun)

Bottom Line: After the most effective dose of Pr was determined in Phase I, Groups 11-13 in Phase II received 10 mg/kg Pr plus either 0.25, 0.5, or 1 mg/kg, IM estradiol valerate every 5 days for four doses.Administration of Pr (10 mg/kg) plus CP decreased KTDS and BW loss and KW.Co-administration of ES/Pr at specific doses improved Cr, BUN, and KTDS; and resulted in reduced CP-induced nephrotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Recently, we reported that estrogen (Es) has no beneficial effect on cisplatin (CP)-induced nephrotoxicity, but the role of progesterone (Pr) and the combination of Es and Pr are not yet well-defined. In this study, we investigated the protective role of Pr, and co-administration of Es/Pr on CP-induced nephrotoxicity. Eighty-six ovariectomized female Wistar rats were divided into 13 groups, and the experiments were performed in two phases. In Phase I, Groups 1-4 received 2, 5, 10, and 25 mg/kg, IM Pr dissolved in sesame oil every 5 days for four doses. Groups 5-8 had the same treatment regimen as Groups 1-4, but after the third injection the animals also received continuous dose of CP (2.5 mg/kg/day, i.p.) for 8 days. Group 9, as the positive control group, received sesame oil instead of Pr plus CP. Group 10, as the negative control group, received sesame oil instead of Pr. After the most effective dose of Pr was determined in Phase I, Groups 11-13 in Phase II received 10 mg/kg Pr plus either 0.25, 0.5, or 1 mg/kg, IM estradiol valerate every 5 days for four doses. After the third injection, they also received a continuous dose of CP for 8 days. The levels of blood urea nitrogen (BUN) and creatinine (Cr), kidney tissue damage score (KTDS), and kidney weight (KW) increased and body weight (BW) decreased in the positive control group (P < 0.05). Administration of Pr (10 mg/kg) plus CP decreased KTDS and BW loss and KW. Co-administration of ES/Pr at specific doses improved Cr, BUN, and KTDS; and resulted in reduced CP-induced nephrotoxicity. The results obtained suggest that the beneficial effect of Pr on CP-induced nephrotoxicity is dose-dependent. In addition, combination of Es/Pr with a specific dose decreased CP-induced nephrotoxicity.

No MeSH data available.


Related in: MedlinePlus

Kidney tissue images (×100) in all the experimental groups. kidney tissue damage score was based on hyaline cast, debris, vacuolization, flattening and degeneration of tubular cells, and dilatation of tubular lumen and graded from 0 to 4. Groups 9 and 10 as positive and negative control groups showed degree of tubular damage 3 ± 0.22 and 0 ± 0 respectively. The arrows indicated tubular damage
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4862261&req=5

Figure 3: Kidney tissue images (×100) in all the experimental groups. kidney tissue damage score was based on hyaline cast, debris, vacuolization, flattening and degeneration of tubular cells, and dilatation of tubular lumen and graded from 0 to 4. Groups 9 and 10 as positive and negative control groups showed degree of tubular damage 3 ± 0.22 and 0 ± 0 respectively. The arrows indicated tubular damage

Mentions: The level of serum nitrite increased in Group 12 (Pr 10+Es 0.5+CP) compared with the positive control group. Administration of CP increased serum MDA in the positive control group compared with Pr 10 group (P < 0.05). Serum MDA levels decreased significantly in all the groups that received Es plus Pr compared with the positive control group (P < 0.05). Administration of Es/Pr significantly increased the kidney level of MDA in Pr 10+Es 0.5 and Pr 10+Es 1 (P < 0/05). The kidney level of nitrite significantly reduced in all the CP-treated groups compared to Pr 10. Also, Pr 10+CP and Pr 10+Es 500+CP decreased kidney nitrite in comparison with the positive control group [Figure 3].


Nephroprotective effect of estrogen and progesterone combination on cisplatin-induced nephrotoxicity in ovariectomized female rats.

Ghasemi M, Nematbakhsh M, Pezeshki Z, Soltani N, Moeini M, Talebi A - Indian J Nephrol (2016 May-Jun)

Kidney tissue images (×100) in all the experimental groups. kidney tissue damage score was based on hyaline cast, debris, vacuolization, flattening and degeneration of tubular cells, and dilatation of tubular lumen and graded from 0 to 4. Groups 9 and 10 as positive and negative control groups showed degree of tubular damage 3 ± 0.22 and 0 ± 0 respectively. The arrows indicated tubular damage
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4862261&req=5

Figure 3: Kidney tissue images (×100) in all the experimental groups. kidney tissue damage score was based on hyaline cast, debris, vacuolization, flattening and degeneration of tubular cells, and dilatation of tubular lumen and graded from 0 to 4. Groups 9 and 10 as positive and negative control groups showed degree of tubular damage 3 ± 0.22 and 0 ± 0 respectively. The arrows indicated tubular damage
Mentions: The level of serum nitrite increased in Group 12 (Pr 10+Es 0.5+CP) compared with the positive control group. Administration of CP increased serum MDA in the positive control group compared with Pr 10 group (P < 0.05). Serum MDA levels decreased significantly in all the groups that received Es plus Pr compared with the positive control group (P < 0.05). Administration of Es/Pr significantly increased the kidney level of MDA in Pr 10+Es 0.5 and Pr 10+Es 1 (P < 0/05). The kidney level of nitrite significantly reduced in all the CP-treated groups compared to Pr 10. Also, Pr 10+CP and Pr 10+Es 500+CP decreased kidney nitrite in comparison with the positive control group [Figure 3].

Bottom Line: After the most effective dose of Pr was determined in Phase I, Groups 11-13 in Phase II received 10 mg/kg Pr plus either 0.25, 0.5, or 1 mg/kg, IM estradiol valerate every 5 days for four doses.Administration of Pr (10 mg/kg) plus CP decreased KTDS and BW loss and KW.Co-administration of ES/Pr at specific doses improved Cr, BUN, and KTDS; and resulted in reduced CP-induced nephrotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT
Recently, we reported that estrogen (Es) has no beneficial effect on cisplatin (CP)-induced nephrotoxicity, but the role of progesterone (Pr) and the combination of Es and Pr are not yet well-defined. In this study, we investigated the protective role of Pr, and co-administration of Es/Pr on CP-induced nephrotoxicity. Eighty-six ovariectomized female Wistar rats were divided into 13 groups, and the experiments were performed in two phases. In Phase I, Groups 1-4 received 2, 5, 10, and 25 mg/kg, IM Pr dissolved in sesame oil every 5 days for four doses. Groups 5-8 had the same treatment regimen as Groups 1-4, but after the third injection the animals also received continuous dose of CP (2.5 mg/kg/day, i.p.) for 8 days. Group 9, as the positive control group, received sesame oil instead of Pr plus CP. Group 10, as the negative control group, received sesame oil instead of Pr. After the most effective dose of Pr was determined in Phase I, Groups 11-13 in Phase II received 10 mg/kg Pr plus either 0.25, 0.5, or 1 mg/kg, IM estradiol valerate every 5 days for four doses. After the third injection, they also received a continuous dose of CP for 8 days. The levels of blood urea nitrogen (BUN) and creatinine (Cr), kidney tissue damage score (KTDS), and kidney weight (KW) increased and body weight (BW) decreased in the positive control group (P < 0.05). Administration of Pr (10 mg/kg) plus CP decreased KTDS and BW loss and KW. Co-administration of ES/Pr at specific doses improved Cr, BUN, and KTDS; and resulted in reduced CP-induced nephrotoxicity. The results obtained suggest that the beneficial effect of Pr on CP-induced nephrotoxicity is dose-dependent. In addition, combination of Es/Pr with a specific dose decreased CP-induced nephrotoxicity.

No MeSH data available.


Related in: MedlinePlus