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Th1, Th2 and Treg/T17 cytokines in two types of proliferative glomerulonephritis.

Stangou M, Bantis C, Skoularopoulou M, Korelidou L, Kouloukouriotou D, Scina M, Labropoulou IT, Kouri NM, Papagianni A, Efstratiadis G - Indian J Nephrol (2016 May-Jun)

Bottom Line: Urinary excretion of Th1, Th2 and Treg/Th17 cytokines were significantly higher in FSNGN compared to IgAN patients.Th1, Th2 and T17 cytokines were directly implicated in renal pathology in IgAN and possibly through MCP-1 production in FSNGN.IL-17 and IL-6 seem to have a central role in inflammation and progression of kidney injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Aristotle University, Hippokration Hospital, Thessaloniki, Greece.

ABSTRACT
IgA nephropathy (IgAN) and focal segmental necrotizing glomerulonephritis (FSNGN) are characterized by proliferation of native glomerular cells and infiltration by inflammatory cells. Several cytokines act as mediators of kidney damage in both diseases. The aim of the present study was to investigate the role of Th1, Th2 and Treg/T17 cytokines in these types of proliferative glomerulonephritis. Simultaneous measurement of Th1 interleukin (IL-2, IL-12, tumor necrosis factor-alpha [TNF-α], interferon-gamma [INF-γ]), Th2 (IL-4, IL-5, IL-6, IL-10, IL-13), Treg/T17 transforming growth factor-beta 1 (TGF-β1, granulocyte-macrophage colony-stimulating factor [GM-CSF], IL-17) cytokines and C-C chemokines Monocyte chemoattractant protein-1 (MCP-1, macrophage inflammatory protein-1 [MIP-1] β) was performed in first-morning urine samples, at the day of renal biopsy, using a multiplex cytokine assay. Cytokine concentrations were correlated with histological findings and renal function outcome. Urinary excretion of Th1, Th2 and Treg/Th17 cytokines were significantly higher in FSNGN compared to IgAN patients. In IgAN patients (n = 50, M/F: 36/14, M age: 40.7 [17-67] years), Th1, Th2 and T17 cytokines correlated significantly with the presence of endocapillary proliferation, while in FSNGN patients (n = 40, M/F: 24/16, M age: 56.5 [25-80] years), MCP-1 and TGF-β1 had a positive correlation with severe extracapillary proliferation (P = 0.001 and P = 0.002, respectively). Urinary IL-17 was the only independent parameter associated with endocapillary proliferation in IgAN and with MCP-1 urinary excretion in FSNGN. Response to treatment was mainly predicted by IL-6 in IgAN, and by Th2 (IL-4, IL-6), Treg (GM-CSF) cytokines and MIP-1 β in FSNGN. Th1, Th2 and T17 cytokines were directly implicated in renal pathology in IgAN and possibly through MCP-1 production in FSNGN. IL-17 and IL-6 seem to have a central role in inflammation and progression of kidney injury.

No MeSH data available.


Related in: MedlinePlus

Differences in urinary cytokine excretion in focal segmental necrotizing glomerulonephritis according to the severity of tubular atrophy and crescent formation
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Figure 2: Differences in urinary cytokine excretion in focal segmental necrotizing glomerulonephritis according to the severity of tubular atrophy and crescent formation

Mentions: Kidney biopsies showed global sclerosis in 20.4% ±19.8% of glomeruli, crescent formation in 68.9% ±25.9% of glomeruli, severe tubular atrophy (≥50% of tubules) in 16/40 (40%) patients. The only finding in renal histology which correlated significantly with renal function outcome was the percentage of crescents (r = −0.6, P = 0.003). Patients with crescents in ≥50% of glomeruli and patients with tubular atrophy in ≥50% of tubules had significantly increased urinary levels of MCP-1 (29 ± 32 vs. 0 fg/mg Ucr, P = 0.001 and 28.8 ± 10.7 vs. 0 fg/mg Ucr, P = 0.001, respectively) and TGF-β1 (0.9 ± 1.5 vs. 0.09 ± 0.09 fg/mg Ucr, P = 0.01 and 1.1 ± 1.5 vs. 0.15 ± 0.17 fg/mg Ucr, P = 0.002, respectively) [Figure 2]. There was no difference in urinary cytokine excretion between PR3 and MPO-ANCA (+) patients.


Th1, Th2 and Treg/T17 cytokines in two types of proliferative glomerulonephritis.

Stangou M, Bantis C, Skoularopoulou M, Korelidou L, Kouloukouriotou D, Scina M, Labropoulou IT, Kouri NM, Papagianni A, Efstratiadis G - Indian J Nephrol (2016 May-Jun)

Differences in urinary cytokine excretion in focal segmental necrotizing glomerulonephritis according to the severity of tubular atrophy and crescent formation
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4862260&req=5

Figure 2: Differences in urinary cytokine excretion in focal segmental necrotizing glomerulonephritis according to the severity of tubular atrophy and crescent formation
Mentions: Kidney biopsies showed global sclerosis in 20.4% ±19.8% of glomeruli, crescent formation in 68.9% ±25.9% of glomeruli, severe tubular atrophy (≥50% of tubules) in 16/40 (40%) patients. The only finding in renal histology which correlated significantly with renal function outcome was the percentage of crescents (r = −0.6, P = 0.003). Patients with crescents in ≥50% of glomeruli and patients with tubular atrophy in ≥50% of tubules had significantly increased urinary levels of MCP-1 (29 ± 32 vs. 0 fg/mg Ucr, P = 0.001 and 28.8 ± 10.7 vs. 0 fg/mg Ucr, P = 0.001, respectively) and TGF-β1 (0.9 ± 1.5 vs. 0.09 ± 0.09 fg/mg Ucr, P = 0.01 and 1.1 ± 1.5 vs. 0.15 ± 0.17 fg/mg Ucr, P = 0.002, respectively) [Figure 2]. There was no difference in urinary cytokine excretion between PR3 and MPO-ANCA (+) patients.

Bottom Line: Urinary excretion of Th1, Th2 and Treg/Th17 cytokines were significantly higher in FSNGN compared to IgAN patients.Th1, Th2 and T17 cytokines were directly implicated in renal pathology in IgAN and possibly through MCP-1 production in FSNGN.IL-17 and IL-6 seem to have a central role in inflammation and progression of kidney injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Aristotle University, Hippokration Hospital, Thessaloniki, Greece.

ABSTRACT
IgA nephropathy (IgAN) and focal segmental necrotizing glomerulonephritis (FSNGN) are characterized by proliferation of native glomerular cells and infiltration by inflammatory cells. Several cytokines act as mediators of kidney damage in both diseases. The aim of the present study was to investigate the role of Th1, Th2 and Treg/T17 cytokines in these types of proliferative glomerulonephritis. Simultaneous measurement of Th1 interleukin (IL-2, IL-12, tumor necrosis factor-alpha [TNF-α], interferon-gamma [INF-γ]), Th2 (IL-4, IL-5, IL-6, IL-10, IL-13), Treg/T17 transforming growth factor-beta 1 (TGF-β1, granulocyte-macrophage colony-stimulating factor [GM-CSF], IL-17) cytokines and C-C chemokines Monocyte chemoattractant protein-1 (MCP-1, macrophage inflammatory protein-1 [MIP-1] β) was performed in first-morning urine samples, at the day of renal biopsy, using a multiplex cytokine assay. Cytokine concentrations were correlated with histological findings and renal function outcome. Urinary excretion of Th1, Th2 and Treg/Th17 cytokines were significantly higher in FSNGN compared to IgAN patients. In IgAN patients (n = 50, M/F: 36/14, M age: 40.7 [17-67] years), Th1, Th2 and T17 cytokines correlated significantly with the presence of endocapillary proliferation, while in FSNGN patients (n = 40, M/F: 24/16, M age: 56.5 [25-80] years), MCP-1 and TGF-β1 had a positive correlation with severe extracapillary proliferation (P = 0.001 and P = 0.002, respectively). Urinary IL-17 was the only independent parameter associated with endocapillary proliferation in IgAN and with MCP-1 urinary excretion in FSNGN. Response to treatment was mainly predicted by IL-6 in IgAN, and by Th2 (IL-4, IL-6), Treg (GM-CSF) cytokines and MIP-1 β in FSNGN. Th1, Th2 and T17 cytokines were directly implicated in renal pathology in IgAN and possibly through MCP-1 production in FSNGN. IL-17 and IL-6 seem to have a central role in inflammation and progression of kidney injury.

No MeSH data available.


Related in: MedlinePlus