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Human Mesenchymal Stem Cell Morphology and Migration on Microtextured Titanium.

Banik BL, Riley TR, Platt CJ, Brown JL - Front Bioeng Biotechnol (2016)

Bottom Line: This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces.The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface.Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Musculoskeletal Regenerative Engineering Laboratory, Department of Biomedical Engineering, The Pennsylvania State University , University Park, PA , USA.

ABSTRACT
The implant used in spinal fusion procedures is an essential component to achieving successful arthrodesis. At the cellular level, the implant impacts healing and fusion through a series of steps: first, mesenchymal stem cells (MSCs) need to adhere and proliferate to cover the implant; second, the MSCs must differentiate into osteoblasts; third, the osteoid matrix produced by the osteoblasts needs to generate new bone tissue, thoroughly integrating the implant with the vertebrate above and below. Previous research has demonstrated that microtextured titanium is advantageous over smooth titanium and PEEK implants for both promoting osteogenic differentiation and integrating with host bone tissue; however, no investigation to date has examined the early morphology and migration of MSCs on these surfaces. This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces. The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface. Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

No MeSH data available.


Related in: MedlinePlus

Stem cell migration on each surface was assessed from 6 to 18 h post-seeding. The results demonstrate random migration on the PEEK and acid-etched endoskeleton surfaces indicated by the rose plots in (A), a histogram of the angle of migration for each cell monitored in (B), and the graph of directionality in (C), which demonstrates significance between PEEK and acid-etched endoskeleton surfaces when compared to the smooth titanium surface. Furthermore, the non-random migration on smooth titanium followed the grooves created by milling the surface, and this non-random migration resulted in an expected velocity increase, which was significantly higher than both the PEEK and acid-etched endoskeleton surfaces. Between the two surfaces demonstrating random migration, the MSCs on the acid-etched endoskeleton surface demonstrated a significantly higher velocity than those on PEEK. Significance, p < 0.05, is demonstrated by bars between groups in (B,C).
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Figure 4: Stem cell migration on each surface was assessed from 6 to 18 h post-seeding. The results demonstrate random migration on the PEEK and acid-etched endoskeleton surfaces indicated by the rose plots in (A), a histogram of the angle of migration for each cell monitored in (B), and the graph of directionality in (C), which demonstrates significance between PEEK and acid-etched endoskeleton surfaces when compared to the smooth titanium surface. Furthermore, the non-random migration on smooth titanium followed the grooves created by milling the surface, and this non-random migration resulted in an expected velocity increase, which was significantly higher than both the PEEK and acid-etched endoskeleton surfaces. Between the two surfaces demonstrating random migration, the MSCs on the acid-etched endoskeleton surface demonstrated a significantly higher velocity than those on PEEK. Significance, p < 0.05, is demonstrated by bars between groups in (B,C).

Mentions: In addition to the morphology shift on each of the three surfaces, the rate and direction of migration were assayed beginning at 6 h post-seeding and continuing for 12 h. Figure 4 depicts the quantitative migration data. In Figure 4A, rose plots (circular histograms) are provided to demonstrate the direction of travel. The MSCs on both smooth surfaces (e.g., PEEK and Ti) demonstrate migration along predominantly one axis. In contrast, the MSCs on the acid-etched endoskeleton surface demonstrate migration in multiple directions. The velocity on the samples is depicted in Figure 4B. The highest average velocity was found in MSCs on the smooth Ti, 28.24 ± 1.62 μm/h, followed by the acid-etched endoskeleton, 21.39 ± 1.38 μm/h, and the lowest average velocity was observed on smooth PEEK, 16.16 ± 1.46 μm/h. Velocities on each surface were significantly different than all other surfaces. Finally, the directionality of the cells on each surface was measured. Directionality was defined asDirectionality=end-to-end distancetotal distance


Human Mesenchymal Stem Cell Morphology and Migration on Microtextured Titanium.

Banik BL, Riley TR, Platt CJ, Brown JL - Front Bioeng Biotechnol (2016)

Stem cell migration on each surface was assessed from 6 to 18 h post-seeding. The results demonstrate random migration on the PEEK and acid-etched endoskeleton surfaces indicated by the rose plots in (A), a histogram of the angle of migration for each cell monitored in (B), and the graph of directionality in (C), which demonstrates significance between PEEK and acid-etched endoskeleton surfaces when compared to the smooth titanium surface. Furthermore, the non-random migration on smooth titanium followed the grooves created by milling the surface, and this non-random migration resulted in an expected velocity increase, which was significantly higher than both the PEEK and acid-etched endoskeleton surfaces. Between the two surfaces demonstrating random migration, the MSCs on the acid-etched endoskeleton surface demonstrated a significantly higher velocity than those on PEEK. Significance, p < 0.05, is demonstrated by bars between groups in (B,C).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4862254&req=5

Figure 4: Stem cell migration on each surface was assessed from 6 to 18 h post-seeding. The results demonstrate random migration on the PEEK and acid-etched endoskeleton surfaces indicated by the rose plots in (A), a histogram of the angle of migration for each cell monitored in (B), and the graph of directionality in (C), which demonstrates significance between PEEK and acid-etched endoskeleton surfaces when compared to the smooth titanium surface. Furthermore, the non-random migration on smooth titanium followed the grooves created by milling the surface, and this non-random migration resulted in an expected velocity increase, which was significantly higher than both the PEEK and acid-etched endoskeleton surfaces. Between the two surfaces demonstrating random migration, the MSCs on the acid-etched endoskeleton surface demonstrated a significantly higher velocity than those on PEEK. Significance, p < 0.05, is demonstrated by bars between groups in (B,C).
Mentions: In addition to the morphology shift on each of the three surfaces, the rate and direction of migration were assayed beginning at 6 h post-seeding and continuing for 12 h. Figure 4 depicts the quantitative migration data. In Figure 4A, rose plots (circular histograms) are provided to demonstrate the direction of travel. The MSCs on both smooth surfaces (e.g., PEEK and Ti) demonstrate migration along predominantly one axis. In contrast, the MSCs on the acid-etched endoskeleton surface demonstrate migration in multiple directions. The velocity on the samples is depicted in Figure 4B. The highest average velocity was found in MSCs on the smooth Ti, 28.24 ± 1.62 μm/h, followed by the acid-etched endoskeleton, 21.39 ± 1.38 μm/h, and the lowest average velocity was observed on smooth PEEK, 16.16 ± 1.46 μm/h. Velocities on each surface were significantly different than all other surfaces. Finally, the directionality of the cells on each surface was measured. Directionality was defined asDirectionality=end-to-end distancetotal distance

Bottom Line: This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces.The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface.Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Musculoskeletal Regenerative Engineering Laboratory, Department of Biomedical Engineering, The Pennsylvania State University , University Park, PA , USA.

ABSTRACT
The implant used in spinal fusion procedures is an essential component to achieving successful arthrodesis. At the cellular level, the implant impacts healing and fusion through a series of steps: first, mesenchymal stem cells (MSCs) need to adhere and proliferate to cover the implant; second, the MSCs must differentiate into osteoblasts; third, the osteoid matrix produced by the osteoblasts needs to generate new bone tissue, thoroughly integrating the implant with the vertebrate above and below. Previous research has demonstrated that microtextured titanium is advantageous over smooth titanium and PEEK implants for both promoting osteogenic differentiation and integrating with host bone tissue; however, no investigation to date has examined the early morphology and migration of MSCs on these surfaces. This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces. The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface. Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

No MeSH data available.


Related in: MedlinePlus