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Human Mesenchymal Stem Cell Morphology and Migration on Microtextured Titanium.

Banik BL, Riley TR, Platt CJ, Brown JL - Front Bioeng Biotechnol (2016)

Bottom Line: This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces.The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface.Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Musculoskeletal Regenerative Engineering Laboratory, Department of Biomedical Engineering, The Pennsylvania State University , University Park, PA , USA.

ABSTRACT
The implant used in spinal fusion procedures is an essential component to achieving successful arthrodesis. At the cellular level, the implant impacts healing and fusion through a series of steps: first, mesenchymal stem cells (MSCs) need to adhere and proliferate to cover the implant; second, the MSCs must differentiate into osteoblasts; third, the osteoid matrix produced by the osteoblasts needs to generate new bone tissue, thoroughly integrating the implant with the vertebrate above and below. Previous research has demonstrated that microtextured titanium is advantageous over smooth titanium and PEEK implants for both promoting osteogenic differentiation and integrating with host bone tissue; however, no investigation to date has examined the early morphology and migration of MSCs on these surfaces. This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces. The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface. Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

No MeSH data available.


Related in: MedlinePlus

Morphological changes of mesenchymal stem cells analyzed at 2, 6, and 24 h post-seeding. (A) Area, (B) circularity, and (C) aspect ratio measurements were taken. The results indicate that stem cells on the acid-etched endoskeleton surface spread the most over 24 h. The circularity of the three surfaces began dissimilar, but converged at 24 h. The aspect ratio of stem cells initially began close to 1, but over 24 h, the smooth surfaces, Ti and PEEK, increased significantly higher than the rough, acid-etched endoskeleton surface. Taken together, the aspect ratio and circularity indicate that stem cells on smooth surfaces move toward a spindle or fibroblastic morphology, whereas those on the rough, acid-etched endoskeleton surface moved toward a stellate or star-like morphology. Within a single time point, * indicates significance, p < 0.05 between acid-etched Ti and PEEK, † indicates significance between acid-etched Ti and Ti, and § indicates significance between PEEK and Ti. Color-coded bars demonstrate significance between time points for a single surface.
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Figure 2: Morphological changes of mesenchymal stem cells analyzed at 2, 6, and 24 h post-seeding. (A) Area, (B) circularity, and (C) aspect ratio measurements were taken. The results indicate that stem cells on the acid-etched endoskeleton surface spread the most over 24 h. The circularity of the three surfaces began dissimilar, but converged at 24 h. The aspect ratio of stem cells initially began close to 1, but over 24 h, the smooth surfaces, Ti and PEEK, increased significantly higher than the rough, acid-etched endoskeleton surface. Taken together, the aspect ratio and circularity indicate that stem cells on smooth surfaces move toward a spindle or fibroblastic morphology, whereas those on the rough, acid-etched endoskeleton surface moved toward a stellate or star-like morphology. Within a single time point, * indicates significance, p < 0.05 between acid-etched Ti and PEEK, † indicates significance between acid-etched Ti and Ti, and § indicates significance between PEEK and Ti. Color-coded bars demonstrate significance between time points for a single surface.

Mentions: Mesenchymal stem cell morphology was examined both quantitatively from 2 to 24 h and qualitatively at 24 h. Figure 2 presents the quantitative cell morphology results on smooth PEEK, smooth Ti, and acid-etched endoskeleton surfaces. Figure 2A presents the areas of MSCs on each surface and shows an increasing trend for MSCs on the acid-etched endoskeleton surface: 6801 ± 533 μm2 at 2 h, 7016 ± 647 μm2 at 6 h, and ending with 8795 ± 841 μm2 at 24 h. Similarly, the spreading area of MSCs on the smooth Ti increased at each time point: 5047 ± 634 μm2 at 2 h, 5971 ± 562 μm2 at 6 h, and 6041 ± 396 μm2 at 24 h. However, the smooth PEEK was the only surface to demonstrate a maximal value followed by a decrease: 5292 ± 442 μm2 at 2 h, 7008 ± 702 μm2 at 6 h, and falling back to 5791 ± 565 μm2 at 24 h. Only the acid-etched endoskeleton surface demonstrated significance with respect to area, demonstrating more spreading area per cell at 24 h when compared to the other surfaces at 24 h in addition to the earlier time points on the acid-etched surface. Next, the circularity of MSCs on the surfaces was analyzed. Circularity was defined asCircularity=4πAP2


Human Mesenchymal Stem Cell Morphology and Migration on Microtextured Titanium.

Banik BL, Riley TR, Platt CJ, Brown JL - Front Bioeng Biotechnol (2016)

Morphological changes of mesenchymal stem cells analyzed at 2, 6, and 24 h post-seeding. (A) Area, (B) circularity, and (C) aspect ratio measurements were taken. The results indicate that stem cells on the acid-etched endoskeleton surface spread the most over 24 h. The circularity of the three surfaces began dissimilar, but converged at 24 h. The aspect ratio of stem cells initially began close to 1, but over 24 h, the smooth surfaces, Ti and PEEK, increased significantly higher than the rough, acid-etched endoskeleton surface. Taken together, the aspect ratio and circularity indicate that stem cells on smooth surfaces move toward a spindle or fibroblastic morphology, whereas those on the rough, acid-etched endoskeleton surface moved toward a stellate or star-like morphology. Within a single time point, * indicates significance, p < 0.05 between acid-etched Ti and PEEK, † indicates significance between acid-etched Ti and Ti, and § indicates significance between PEEK and Ti. Color-coded bars demonstrate significance between time points for a single surface.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4862254&req=5

Figure 2: Morphological changes of mesenchymal stem cells analyzed at 2, 6, and 24 h post-seeding. (A) Area, (B) circularity, and (C) aspect ratio measurements were taken. The results indicate that stem cells on the acid-etched endoskeleton surface spread the most over 24 h. The circularity of the three surfaces began dissimilar, but converged at 24 h. The aspect ratio of stem cells initially began close to 1, but over 24 h, the smooth surfaces, Ti and PEEK, increased significantly higher than the rough, acid-etched endoskeleton surface. Taken together, the aspect ratio and circularity indicate that stem cells on smooth surfaces move toward a spindle or fibroblastic morphology, whereas those on the rough, acid-etched endoskeleton surface moved toward a stellate or star-like morphology. Within a single time point, * indicates significance, p < 0.05 between acid-etched Ti and PEEK, † indicates significance between acid-etched Ti and Ti, and § indicates significance between PEEK and Ti. Color-coded bars demonstrate significance between time points for a single surface.
Mentions: Mesenchymal stem cell morphology was examined both quantitatively from 2 to 24 h and qualitatively at 24 h. Figure 2 presents the quantitative cell morphology results on smooth PEEK, smooth Ti, and acid-etched endoskeleton surfaces. Figure 2A presents the areas of MSCs on each surface and shows an increasing trend for MSCs on the acid-etched endoskeleton surface: 6801 ± 533 μm2 at 2 h, 7016 ± 647 μm2 at 6 h, and ending with 8795 ± 841 μm2 at 24 h. Similarly, the spreading area of MSCs on the smooth Ti increased at each time point: 5047 ± 634 μm2 at 2 h, 5971 ± 562 μm2 at 6 h, and 6041 ± 396 μm2 at 24 h. However, the smooth PEEK was the only surface to demonstrate a maximal value followed by a decrease: 5292 ± 442 μm2 at 2 h, 7008 ± 702 μm2 at 6 h, and falling back to 5791 ± 565 μm2 at 24 h. Only the acid-etched endoskeleton surface demonstrated significance with respect to area, demonstrating more spreading area per cell at 24 h when compared to the other surfaces at 24 h in addition to the earlier time points on the acid-etched surface. Next, the circularity of MSCs on the surfaces was analyzed. Circularity was defined asCircularity=4πAP2

Bottom Line: This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces.The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface.Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Musculoskeletal Regenerative Engineering Laboratory, Department of Biomedical Engineering, The Pennsylvania State University , University Park, PA , USA.

ABSTRACT
The implant used in spinal fusion procedures is an essential component to achieving successful arthrodesis. At the cellular level, the implant impacts healing and fusion through a series of steps: first, mesenchymal stem cells (MSCs) need to adhere and proliferate to cover the implant; second, the MSCs must differentiate into osteoblasts; third, the osteoid matrix produced by the osteoblasts needs to generate new bone tissue, thoroughly integrating the implant with the vertebrate above and below. Previous research has demonstrated that microtextured titanium is advantageous over smooth titanium and PEEK implants for both promoting osteogenic differentiation and integrating with host bone tissue; however, no investigation to date has examined the early morphology and migration of MSCs on these surfaces. This study details cell spreading and morphology changes over 24 h, rate and directionality of migration 6-18 h post-seeding, differentiation markers at 10 days, and the long-term morphology of MSCs at 7 days, on microtextured, acid-etched titanium (endoskeleton), smooth titanium, and smooth PEEK surfaces. The results demonstrate that in all metrics, the two titanium surfaces outperformed the PEEK surface. Furthermore, the rough acid-etched titanium surface presented the most favorable overall results, demonstrating the random migration needed to efficiently cover a surface in addition to morphologies consistent with osteoblasts and preosteoblasts.

No MeSH data available.


Related in: MedlinePlus