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Evaluation of ELISA for the detection of rabies virus antibodies from the thoracic liquid and muscle extract samples in the monitoring of fox oral vaccination campaigns.

Bedeković T, Šimić I, Krešić N, Lojkić I, Mihaljević Ž, Sučec I, Janković IL, Hostnik P - BMC Vet. Res. (2016)

Bottom Line: Also, the influence of heat treatment of samples on test results was investigated.Diagnostic validity of the ELISA compared to the mFAVN test when not-heat treated samples were used was 89.16%.Diagnostic validity of the BioPro ELISA compared to the mFAVN test for heat treated samples was 94.30%.

View Article: PubMed Central - PubMed

Affiliation: Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia. bedekovic@veinst.hr.

ABSTRACT

Background: The main goal of oral vaccination of foxes is eradication of rabies in the red fox population as rabies reservoirs. To evaluate the success of vaccination a serological testing is conducted as a part of monitoring program. Two different methods are used regarding rabies serology: virus neutralisation test and ELISA.

Methods: In this study the reliability of BioPro ELISA was evaluated for testing haemolytic thoracic liquids and muscle extracts originated from 147 foxes in comparison to mFAVN. Also, the influence of heat treatment of samples on test results was investigated.

Results: The specificity of the test for not-heat treated samples was 92.98% and sensitivity 79.20%. Diagnostic validity of the ELISA compared to the mFAVN test when not-heat treated samples were used was 89.16%. The specificity of the test for heat treated samples was 79.10% and sensitivity 96.36%. Diagnostic validity of the BioPro ELISA compared to the mFAVN test for heat treated samples was 94.30%.

Conclusion: According to this study, the BioPro ELISA is reliable tool for detection of rabies specific antibodies in the context of evaluation of oral vaccination of foxes from poor quality samples as a substitution for virus neutralisation tests.

No MeSH data available.


Related in: MedlinePlus

a Correlation between titres of antibodies (IU/ml) in not-heat treated muscle extracts samples titrated by mFAVN test (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %). b Correlation between titres of antibodies (IU/ml) in heat treated muscle extracts samples titrated by mFAVN (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %)
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Fig3: a Correlation between titres of antibodies (IU/ml) in not-heat treated muscle extracts samples titrated by mFAVN test (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %). b Correlation between titres of antibodies (IU/ml) in heat treated muscle extracts samples titrated by mFAVN (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %)

Mentions: The results regarding sensitivity, specificity, diagnostic agreement and diagnostic validity for either type of samples are recorded in Table 3. Correlation between titres of antibodies titrated by mFAVN test and percent of blocking obtained with BioPro ELISA are recorded in Figs. 1, 2 and 3.Table 3


Evaluation of ELISA for the detection of rabies virus antibodies from the thoracic liquid and muscle extract samples in the monitoring of fox oral vaccination campaigns.

Bedeković T, Šimić I, Krešić N, Lojkić I, Mihaljević Ž, Sučec I, Janković IL, Hostnik P - BMC Vet. Res. (2016)

a Correlation between titres of antibodies (IU/ml) in not-heat treated muscle extracts samples titrated by mFAVN test (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %). b Correlation between titres of antibodies (IU/ml) in heat treated muscle extracts samples titrated by mFAVN (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862130&req=5

Fig3: a Correlation between titres of antibodies (IU/ml) in not-heat treated muscle extracts samples titrated by mFAVN test (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %). b Correlation between titres of antibodies (IU/ml) in heat treated muscle extracts samples titrated by mFAVN (cut off was set on 0.1 IU/ml) and percent of blocking obtained with BioPro ELISA (cut off was set on 40 %)
Mentions: The results regarding sensitivity, specificity, diagnostic agreement and diagnostic validity for either type of samples are recorded in Table 3. Correlation between titres of antibodies titrated by mFAVN test and percent of blocking obtained with BioPro ELISA are recorded in Figs. 1, 2 and 3.Table 3

Bottom Line: Also, the influence of heat treatment of samples on test results was investigated.Diagnostic validity of the ELISA compared to the mFAVN test when not-heat treated samples were used was 89.16%.Diagnostic validity of the BioPro ELISA compared to the mFAVN test for heat treated samples was 94.30%.

View Article: PubMed Central - PubMed

Affiliation: Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia. bedekovic@veinst.hr.

ABSTRACT

Background: The main goal of oral vaccination of foxes is eradication of rabies in the red fox population as rabies reservoirs. To evaluate the success of vaccination a serological testing is conducted as a part of monitoring program. Two different methods are used regarding rabies serology: virus neutralisation test and ELISA.

Methods: In this study the reliability of BioPro ELISA was evaluated for testing haemolytic thoracic liquids and muscle extracts originated from 147 foxes in comparison to mFAVN. Also, the influence of heat treatment of samples on test results was investigated.

Results: The specificity of the test for not-heat treated samples was 92.98% and sensitivity 79.20%. Diagnostic validity of the ELISA compared to the mFAVN test when not-heat treated samples were used was 89.16%. The specificity of the test for heat treated samples was 79.10% and sensitivity 96.36%. Diagnostic validity of the BioPro ELISA compared to the mFAVN test for heat treated samples was 94.30%.

Conclusion: According to this study, the BioPro ELISA is reliable tool for detection of rabies specific antibodies in the context of evaluation of oral vaccination of foxes from poor quality samples as a substitution for virus neutralisation tests.

No MeSH data available.


Related in: MedlinePlus