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Borrelia persica infection in dogs and cats: clinical manifestations, clinicopathological findings and genetic characterization.

Baneth G, Nachum-Biala Y, Halperin T, Hershko Y, Kleinerman G, Anug Y, Abdeen Z, Lavy E, Aroch I, Straubinger RK - Parasit Vectors (2016)

Bottom Line: The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.Infection was associated with anemia and thrombocytopenia.Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

View Article: PubMed Central - PubMed

Affiliation: Koret School of Veterinary Medicine, Hebrew University, Rehovot, Israel. gad.baneth@mail.huji.ac.il.

ABSTRACT

Background: Relapsing fever (RF) is an acute infectious disease caused by arthropod-borne spirochetes of the genus Borrelia. The disease is characterized by recurrent episodes of fever that concur with spirochetemia. The RF borrelioses include louse-borne RF caused by Borrelia recurrentis and tick-borne endemic RF transmitted by argasid soft ticks and caused by several Borrelia spp. such as B. crocidurae, B. coriaceae, B. duttoni, B. hermsii, B. hispanica and B. persica. Human infection with B. persica is transmitted by the soft tick Ornithodoros tholozani and has been reported from Iran, Israel, Egypt, India, and Central Asia.

Methods: During 2003-2015, five cats and five dogs from northern, central and southern Israel were presented for veterinary care and detected with borrelia spirochetemia by blood smear microscopy. The causative infective agent in these animals was identified and characterized by PCR from blood and sequencing of parts of the flagellin (flab), 16S rRNA and glycerophosphodiester phosphodiestrase (GlpQ) genes.

Results: All animals were infected with B. persica genetically identical to the causative agent of human RF. Phylogenetic analysis indicated that DNA sequences from these pet carnivores clustered together with B. persica genotypes I and II from humans and O. tholozani ticks and distinctly from other RF Borrelia spp. The main clinical findings in cats included lethargy, anorexia, anemia in 5/5 cats and thrombocytopenia in 4/5. All dogs were lethargic and anorectic, 4/5 were febrile and anemic and 3/5 were thrombocytopenic. Three dogs were co-infected with Babesia spp. The animals were all treated with antibiotics and the survival rate of both dogs and cats was 80 %. The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.

Conclusions: This is the first report of disease due to B. persica infection in cats and the first case series in dogs. Infection was associated with anemia and thrombocytopenia. Fever was more frequently observed in dogs than cats. Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

No MeSH data available.


Related in: MedlinePlus

A maximum likelihood phylogram comparing 267 bp DNA sequences of the flaB gene from the cats and dogs included in the study to sequences from other B. persica GenBank accessions and from other Borrelia spp. New sequences derived from this study are marked with black diamond squares. Note the division into B. persica genotypes marked in Roman numerals. The GenBank accession numbers, species of infected host and country of origin are included for each sequence. The Tamura-3-Parameter model was used in the construction of this phylogram and bootstrap values higher than 70 % are indicated
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Fig6: A maximum likelihood phylogram comparing 267 bp DNA sequences of the flaB gene from the cats and dogs included in the study to sequences from other B. persica GenBank accessions and from other Borrelia spp. New sequences derived from this study are marked with black diamond squares. Note the division into B. persica genotypes marked in Roman numerals. The GenBank accession numbers, species of infected host and country of origin are included for each sequence. The Tamura-3-Parameter model was used in the construction of this phylogram and bootstrap values higher than 70 % are indicated

Mentions: Phylogenetic analysis of the flaB gene sequences (Fig. 6) indicated that the sequences from the B. persica infected dogs and cats clustered together with each other and with sequences of other B. persica organisms recovered from humans and O. tholozani ticks. Most of the cat and dog sequences clustered together with a B. persica genotype 1 sequence (DQ679907) from a human individual, whereas one cat sequence clustered together with a B. persica genotype 2 from a tick (DQ6795509), while none of our sequences clustered closely with B. persica genotype 3 from a human (DQ679906). All B. persica sequences clustered separately from other Old World RF Borrelia spp. including B. duttonii, B. crociduare, B. hispanica and B. recurrentis. RF species from the American continent including B. parkeri, B. turicatae and B. hermsii also clustered separately and together with B. miyamotoi which was described from the American continent and also from Asia and Europe.Fig. 6


Borrelia persica infection in dogs and cats: clinical manifestations, clinicopathological findings and genetic characterization.

Baneth G, Nachum-Biala Y, Halperin T, Hershko Y, Kleinerman G, Anug Y, Abdeen Z, Lavy E, Aroch I, Straubinger RK - Parasit Vectors (2016)

A maximum likelihood phylogram comparing 267 bp DNA sequences of the flaB gene from the cats and dogs included in the study to sequences from other B. persica GenBank accessions and from other Borrelia spp. New sequences derived from this study are marked with black diamond squares. Note the division into B. persica genotypes marked in Roman numerals. The GenBank accession numbers, species of infected host and country of origin are included for each sequence. The Tamura-3-Parameter model was used in the construction of this phylogram and bootstrap values higher than 70 % are indicated
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862127&req=5

Fig6: A maximum likelihood phylogram comparing 267 bp DNA sequences of the flaB gene from the cats and dogs included in the study to sequences from other B. persica GenBank accessions and from other Borrelia spp. New sequences derived from this study are marked with black diamond squares. Note the division into B. persica genotypes marked in Roman numerals. The GenBank accession numbers, species of infected host and country of origin are included for each sequence. The Tamura-3-Parameter model was used in the construction of this phylogram and bootstrap values higher than 70 % are indicated
Mentions: Phylogenetic analysis of the flaB gene sequences (Fig. 6) indicated that the sequences from the B. persica infected dogs and cats clustered together with each other and with sequences of other B. persica organisms recovered from humans and O. tholozani ticks. Most of the cat and dog sequences clustered together with a B. persica genotype 1 sequence (DQ679907) from a human individual, whereas one cat sequence clustered together with a B. persica genotype 2 from a tick (DQ6795509), while none of our sequences clustered closely with B. persica genotype 3 from a human (DQ679906). All B. persica sequences clustered separately from other Old World RF Borrelia spp. including B. duttonii, B. crociduare, B. hispanica and B. recurrentis. RF species from the American continent including B. parkeri, B. turicatae and B. hermsii also clustered separately and together with B. miyamotoi which was described from the American continent and also from Asia and Europe.Fig. 6

Bottom Line: The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.Infection was associated with anemia and thrombocytopenia.Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

View Article: PubMed Central - PubMed

Affiliation: Koret School of Veterinary Medicine, Hebrew University, Rehovot, Israel. gad.baneth@mail.huji.ac.il.

ABSTRACT

Background: Relapsing fever (RF) is an acute infectious disease caused by arthropod-borne spirochetes of the genus Borrelia. The disease is characterized by recurrent episodes of fever that concur with spirochetemia. The RF borrelioses include louse-borne RF caused by Borrelia recurrentis and tick-borne endemic RF transmitted by argasid soft ticks and caused by several Borrelia spp. such as B. crocidurae, B. coriaceae, B. duttoni, B. hermsii, B. hispanica and B. persica. Human infection with B. persica is transmitted by the soft tick Ornithodoros tholozani and has been reported from Iran, Israel, Egypt, India, and Central Asia.

Methods: During 2003-2015, five cats and five dogs from northern, central and southern Israel were presented for veterinary care and detected with borrelia spirochetemia by blood smear microscopy. The causative infective agent in these animals was identified and characterized by PCR from blood and sequencing of parts of the flagellin (flab), 16S rRNA and glycerophosphodiester phosphodiestrase (GlpQ) genes.

Results: All animals were infected with B. persica genetically identical to the causative agent of human RF. Phylogenetic analysis indicated that DNA sequences from these pet carnivores clustered together with B. persica genotypes I and II from humans and O. tholozani ticks and distinctly from other RF Borrelia spp. The main clinical findings in cats included lethargy, anorexia, anemia in 5/5 cats and thrombocytopenia in 4/5. All dogs were lethargic and anorectic, 4/5 were febrile and anemic and 3/5 were thrombocytopenic. Three dogs were co-infected with Babesia spp. The animals were all treated with antibiotics and the survival rate of both dogs and cats was 80 %. The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.

Conclusions: This is the first report of disease due to B. persica infection in cats and the first case series in dogs. Infection was associated with anemia and thrombocytopenia. Fever was more frequently observed in dogs than cats. Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

No MeSH data available.


Related in: MedlinePlus