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Borrelia persica infection in dogs and cats: clinical manifestations, clinicopathological findings and genetic characterization.

Baneth G, Nachum-Biala Y, Halperin T, Hershko Y, Kleinerman G, Anug Y, Abdeen Z, Lavy E, Aroch I, Straubinger RK - Parasit Vectors (2016)

Bottom Line: The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.Infection was associated with anemia and thrombocytopenia.Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

View Article: PubMed Central - PubMed

Affiliation: Koret School of Veterinary Medicine, Hebrew University, Rehovot, Israel. gad.baneth@mail.huji.ac.il.

ABSTRACT

Background: Relapsing fever (RF) is an acute infectious disease caused by arthropod-borne spirochetes of the genus Borrelia. The disease is characterized by recurrent episodes of fever that concur with spirochetemia. The RF borrelioses include louse-borne RF caused by Borrelia recurrentis and tick-borne endemic RF transmitted by argasid soft ticks and caused by several Borrelia spp. such as B. crocidurae, B. coriaceae, B. duttoni, B. hermsii, B. hispanica and B. persica. Human infection with B. persica is transmitted by the soft tick Ornithodoros tholozani and has been reported from Iran, Israel, Egypt, India, and Central Asia.

Methods: During 2003-2015, five cats and five dogs from northern, central and southern Israel were presented for veterinary care and detected with borrelia spirochetemia by blood smear microscopy. The causative infective agent in these animals was identified and characterized by PCR from blood and sequencing of parts of the flagellin (flab), 16S rRNA and glycerophosphodiester phosphodiestrase (GlpQ) genes.

Results: All animals were infected with B. persica genetically identical to the causative agent of human RF. Phylogenetic analysis indicated that DNA sequences from these pet carnivores clustered together with B. persica genotypes I and II from humans and O. tholozani ticks and distinctly from other RF Borrelia spp. The main clinical findings in cats included lethargy, anorexia, anemia in 5/5 cats and thrombocytopenia in 4/5. All dogs were lethargic and anorectic, 4/5 were febrile and anemic and 3/5 were thrombocytopenic. Three dogs were co-infected with Babesia spp. The animals were all treated with antibiotics and the survival rate of both dogs and cats was 80 %. The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.

Conclusions: This is the first report of disease due to B. persica infection in cats and the first case series in dogs. Infection was associated with anemia and thrombocytopenia. Fever was more frequently observed in dogs than cats. Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

No MeSH data available.


Related in: MedlinePlus

Higher magnification of spirochetemia with Borrelia persica in cat no. 2. Romanowsky stain. Scale-bar: 10 μM
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Fig3: Higher magnification of spirochetemia with Borrelia persica in cat no. 2. Romanowsky stain. Scale-bar: 10 μM

Mentions: Numerous spirochetes were noted in blood smears from all dogs and cats with 4 to 10 spirochetes per microscopic field at 500× magnification (Fig. 1). Cat #2 which died in the course of infection had an overwhelmingly high spirochetemia (Figs. 2 and 3) and blood obtained from this cat was used for culture of B. persica as previously described [19]. Spirochetes seen in the blood smears occasionally formed aggregates with platelets (Fig. 4) or encircled erythrocytes (Fig. 5).Fig. 1


Borrelia persica infection in dogs and cats: clinical manifestations, clinicopathological findings and genetic characterization.

Baneth G, Nachum-Biala Y, Halperin T, Hershko Y, Kleinerman G, Anug Y, Abdeen Z, Lavy E, Aroch I, Straubinger RK - Parasit Vectors (2016)

Higher magnification of spirochetemia with Borrelia persica in cat no. 2. Romanowsky stain. Scale-bar: 10 μM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862127&req=5

Fig3: Higher magnification of spirochetemia with Borrelia persica in cat no. 2. Romanowsky stain. Scale-bar: 10 μM
Mentions: Numerous spirochetes were noted in blood smears from all dogs and cats with 4 to 10 spirochetes per microscopic field at 500× magnification (Fig. 1). Cat #2 which died in the course of infection had an overwhelmingly high spirochetemia (Figs. 2 and 3) and blood obtained from this cat was used for culture of B. persica as previously described [19]. Spirochetes seen in the blood smears occasionally formed aggregates with platelets (Fig. 4) or encircled erythrocytes (Fig. 5).Fig. 1

Bottom Line: The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.Infection was associated with anemia and thrombocytopenia.Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

View Article: PubMed Central - PubMed

Affiliation: Koret School of Veterinary Medicine, Hebrew University, Rehovot, Israel. gad.baneth@mail.huji.ac.il.

ABSTRACT

Background: Relapsing fever (RF) is an acute infectious disease caused by arthropod-borne spirochetes of the genus Borrelia. The disease is characterized by recurrent episodes of fever that concur with spirochetemia. The RF borrelioses include louse-borne RF caused by Borrelia recurrentis and tick-borne endemic RF transmitted by argasid soft ticks and caused by several Borrelia spp. such as B. crocidurae, B. coriaceae, B. duttoni, B. hermsii, B. hispanica and B. persica. Human infection with B. persica is transmitted by the soft tick Ornithodoros tholozani and has been reported from Iran, Israel, Egypt, India, and Central Asia.

Methods: During 2003-2015, five cats and five dogs from northern, central and southern Israel were presented for veterinary care and detected with borrelia spirochetemia by blood smear microscopy. The causative infective agent in these animals was identified and characterized by PCR from blood and sequencing of parts of the flagellin (flab), 16S rRNA and glycerophosphodiester phosphodiestrase (GlpQ) genes.

Results: All animals were infected with B. persica genetically identical to the causative agent of human RF. Phylogenetic analysis indicated that DNA sequences from these pet carnivores clustered together with B. persica genotypes I and II from humans and O. tholozani ticks and distinctly from other RF Borrelia spp. The main clinical findings in cats included lethargy, anorexia, anemia in 5/5 cats and thrombocytopenia in 4/5. All dogs were lethargic and anorectic, 4/5 were febrile and anemic and 3/5 were thrombocytopenic. Three dogs were co-infected with Babesia spp. The animals were all treated with antibiotics and the survival rate of both dogs and cats was 80 %. The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia.

Conclusions: This is the first report of disease due to B. persica infection in cats and the first case series in dogs. Infection was associated with anemia and thrombocytopenia. Fever was more frequently observed in dogs than cats. Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection.

No MeSH data available.


Related in: MedlinePlus