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A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.


Constitutively expressing TaBASS2 enhances ROS tolerance and reduces Na+ contents in wheat. a DAB staining of the leaves from two-week-old soil-grown vector control (VC) and two Ubi::TaBASS2 lines (OX1 and OX21). b The expression levels of TaCAT1 in two-week-old VC and OX seedlings. c The catalase activity in two-week-old VC and OX seedlings. d The expression levels of TaHKT1;5-D in two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using TaCyclophil in in wheat as the internal control. e, f The Na+ contents in the shoot and root tissue from two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the VC result (P < 0.05, t-test)
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Fig8: Constitutively expressing TaBASS2 enhances ROS tolerance and reduces Na+ contents in wheat. a DAB staining of the leaves from two-week-old soil-grown vector control (VC) and two Ubi::TaBASS2 lines (OX1 and OX21). b The expression levels of TaCAT1 in two-week-old VC and OX seedlings. c The catalase activity in two-week-old VC and OX seedlings. d The expression levels of TaHKT1;5-D in two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using TaCyclophil in in wheat as the internal control. e, f The Na+ contents in the shoot and root tissue from two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the VC result (P < 0.05, t-test)

Mentions: DAB staining determined wheat OX lines had higher H2O2 levels in vivo than the wild-type plants (Fig. 8a). The expression levels of the ROS-scavenging catalase 1 (TaCAT1) were also constitutively up-regulated in the transgenic wheat plants constitutively expressing TaBASS2 (Fig. 8b), along with the CAT1 enzyme activity significantly higher in the transgenic lines (Fig. 8c). These results demonstrated that the constitutive expression of TaBASS2 led to an increase in ROS contents and ROS-scavenging activity in the transgenic wheat plants, suggesting a constitutive activation of ROS signaling. The expression levels of TaHKT1;5-D, the wheat homolog of AtHKT1, were higher in two OX lines than in the wild-type plants (Fig. 8d). The Na+ contents in shoots and roots were lower in those OX lines as well (Fig. 8e and f). These results suggest that TaHKT1;5-D might be regulated to enhance salinity tolerance in transgenic wheat overexpressing TaBASS2.Fig. 8


A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

Constitutively expressing TaBASS2 enhances ROS tolerance and reduces Na+ contents in wheat. a DAB staining of the leaves from two-week-old soil-grown vector control (VC) and two Ubi::TaBASS2 lines (OX1 and OX21). b The expression levels of TaCAT1 in two-week-old VC and OX seedlings. c The catalase activity in two-week-old VC and OX seedlings. d The expression levels of TaHKT1;5-D in two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using TaCyclophil in in wheat as the internal control. e, f The Na+ contents in the shoot and root tissue from two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the VC result (P < 0.05, t-test)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Fig8: Constitutively expressing TaBASS2 enhances ROS tolerance and reduces Na+ contents in wheat. a DAB staining of the leaves from two-week-old soil-grown vector control (VC) and two Ubi::TaBASS2 lines (OX1 and OX21). b The expression levels of TaCAT1 in two-week-old VC and OX seedlings. c The catalase activity in two-week-old VC and OX seedlings. d The expression levels of TaHKT1;5-D in two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using TaCyclophil in in wheat as the internal control. e, f The Na+ contents in the shoot and root tissue from two-week-old VC and OX seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the VC result (P < 0.05, t-test)
Mentions: DAB staining determined wheat OX lines had higher H2O2 levels in vivo than the wild-type plants (Fig. 8a). The expression levels of the ROS-scavenging catalase 1 (TaCAT1) were also constitutively up-regulated in the transgenic wheat plants constitutively expressing TaBASS2 (Fig. 8b), along with the CAT1 enzyme activity significantly higher in the transgenic lines (Fig. 8c). These results demonstrated that the constitutive expression of TaBASS2 led to an increase in ROS contents and ROS-scavenging activity in the transgenic wheat plants, suggesting a constitutive activation of ROS signaling. The expression levels of TaHKT1;5-D, the wheat homolog of AtHKT1, were higher in two OX lines than in the wild-type plants (Fig. 8d). The Na+ contents in shoots and roots were lower in those OX lines as well (Fig. 8e and f). These results suggest that TaHKT1;5-D might be regulated to enhance salinity tolerance in transgenic wheat overexpressing TaBASS2.Fig. 8

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.