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A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.


Related in: MedlinePlus

The enhanced salinity tolerance in 35S::TaBASS2 Arabidopsis plants relies on ABI4 suppression. a-d The wild-type seedlings and two transgenic lines constitutively expressing both TaBASS2 and ABI4 (TaBASS2OE ABI4OE #17 and #19) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 30 seedlings. f, g The expression levels of ABI4 (f) and HKT1;1 (g) in the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 seedlings. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control
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Fig7: The enhanced salinity tolerance in 35S::TaBASS2 Arabidopsis plants relies on ABI4 suppression. a-d The wild-type seedlings and two transgenic lines constitutively expressing both TaBASS2 and ABI4 (TaBASS2OE ABI4OE #17 and #19) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 30 seedlings. f, g The expression levels of ABI4 (f) and HKT1;1 (g) in the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 seedlings. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control

Mentions: As ABI4 was repressed in the OE plants, it was of interest to determine the salinity response when ABI4 remained comparable expression level as in the wild-type. Thus, we carried out a genetic approach to constitutively express ABI4 in 35S::TaBASS2 background. Two transgenic lines (TaBASS2OE ABI4OE #17 from OE1, and TaBASS2OE ABI4OE #19 from OE3) with ABI4 expression levels comparable as in the wild type were selected for phenotypic assays (Fig. 7f). In comparison with the wild-type, both transgenic lines exhibited a similar response to NaCl treatment (Fig. 7a-e), and comparable HKT1;1 expression levels (Fig. 7g). These results demonstrated the enhanced resistance conferred by ectopic expression of TaBASS2 vanished when ABI4 expression was restored, indicating that constitutively expressing TaBASS2 in Arabidopsis conferred enhanced salinity tolerance by repressing ABI4 expression.Fig. 7


A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

The enhanced salinity tolerance in 35S::TaBASS2 Arabidopsis plants relies on ABI4 suppression. a-d The wild-type seedlings and two transgenic lines constitutively expressing both TaBASS2 and ABI4 (TaBASS2OE ABI4OE #17 and #19) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 30 seedlings. f, g The expression levels of ABI4 (f) and HKT1;1 (g) in the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 seedlings. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control
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Fig7: The enhanced salinity tolerance in 35S::TaBASS2 Arabidopsis plants relies on ABI4 suppression. a-d The wild-type seedlings and two transgenic lines constitutively expressing both TaBASS2 and ABI4 (TaBASS2OE ABI4OE #17 and #19) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 30 seedlings. f, g The expression levels of ABI4 (f) and HKT1;1 (g) in the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 seedlings. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control
Mentions: As ABI4 was repressed in the OE plants, it was of interest to determine the salinity response when ABI4 remained comparable expression level as in the wild-type. Thus, we carried out a genetic approach to constitutively express ABI4 in 35S::TaBASS2 background. Two transgenic lines (TaBASS2OE ABI4OE #17 from OE1, and TaBASS2OE ABI4OE #19 from OE3) with ABI4 expression levels comparable as in the wild type were selected for phenotypic assays (Fig. 7f). In comparison with the wild-type, both transgenic lines exhibited a similar response to NaCl treatment (Fig. 7a-e), and comparable HKT1;1 expression levels (Fig. 7g). These results demonstrated the enhanced resistance conferred by ectopic expression of TaBASS2 vanished when ABI4 expression was restored, indicating that constitutively expressing TaBASS2 in Arabidopsis conferred enhanced salinity tolerance by repressing ABI4 expression.Fig. 7

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.


Related in: MedlinePlus