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A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.


Constitutively expressing TaBASS2 enhances ROS content and ROS scavenging activity. a-c The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 1 or 1.5 mM H2O2. Bar = 1 cm. d Relative root growth of the wild-type and OE plants treated with 0, 1 or 1.5 mM H2O2. e DAB staining of the leaves from four-week-old soil-grown wild-type and OE plants. f The expression levels of AtCAT1 in 12-day-old wild-type and OE seedlings. g The catalase activity in 12-day-old wild-type and OE seedlings. Error bars in (d) represent the standard errors (n = 3), with each replicate comprising 30 seedlings. Error bars in (f, g) represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result (P < 0.05, t-test). The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control
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Fig5: Constitutively expressing TaBASS2 enhances ROS content and ROS scavenging activity. a-c The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 1 or 1.5 mM H2O2. Bar = 1 cm. d Relative root growth of the wild-type and OE plants treated with 0, 1 or 1.5 mM H2O2. e DAB staining of the leaves from four-week-old soil-grown wild-type and OE plants. f The expression levels of AtCAT1 in 12-day-old wild-type and OE seedlings. g The catalase activity in 12-day-old wild-type and OE seedlings. Error bars in (d) represent the standard errors (n = 3), with each replicate comprising 30 seedlings. Error bars in (f, g) represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result (P < 0.05, t-test). The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control

Mentions: ROS is involved in plant response to salinity stress. To determine the role of TaBASS2 in oxidative stress response, the 35S::TaBASS2 Arabidposis plant were assessed under H2O2 treatment. As shown in Fig. 5a, b and d, under the treatment of 1 mM H2O2, the relative root growth rate of the wild-type plants was 25 % of that under the control condition, significantly smaller than the relative root growth rates (45 % and 35 %) of OE1 and OE3, respectively. When the H2O2 concentration was raised to 1.5 mM, the relative root growth rates were 22, 40 and 29 % in the wild-type, OE1 and OE3 seedlings, respectively (Fig. 5a, c, d). These results demonstrated that the transgenic Arabidopsis plants constitutively expressing TaBASS2 had higher tolerance to oxidative stress than the wild-type plants did. Moreover, treatment with methyl viologen (MV), which generates superoxide anions in plastids, demonstrated that these OE lines also displayed enhanced tolerance to plastidial oxidative stress (Additional file 6).Fig. 5


A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

Constitutively expressing TaBASS2 enhances ROS content and ROS scavenging activity. a-c The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 1 or 1.5 mM H2O2. Bar = 1 cm. d Relative root growth of the wild-type and OE plants treated with 0, 1 or 1.5 mM H2O2. e DAB staining of the leaves from four-week-old soil-grown wild-type and OE plants. f The expression levels of AtCAT1 in 12-day-old wild-type and OE seedlings. g The catalase activity in 12-day-old wild-type and OE seedlings. Error bars in (d) represent the standard errors (n = 3), with each replicate comprising 30 seedlings. Error bars in (f, g) represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result (P < 0.05, t-test). The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Fig5: Constitutively expressing TaBASS2 enhances ROS content and ROS scavenging activity. a-c The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 1 or 1.5 mM H2O2. Bar = 1 cm. d Relative root growth of the wild-type and OE plants treated with 0, 1 or 1.5 mM H2O2. e DAB staining of the leaves from four-week-old soil-grown wild-type and OE plants. f The expression levels of AtCAT1 in 12-day-old wild-type and OE seedlings. g The catalase activity in 12-day-old wild-type and OE seedlings. Error bars in (d) represent the standard errors (n = 3), with each replicate comprising 30 seedlings. Error bars in (f, g) represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result (P < 0.05, t-test). The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control
Mentions: ROS is involved in plant response to salinity stress. To determine the role of TaBASS2 in oxidative stress response, the 35S::TaBASS2 Arabidposis plant were assessed under H2O2 treatment. As shown in Fig. 5a, b and d, under the treatment of 1 mM H2O2, the relative root growth rate of the wild-type plants was 25 % of that under the control condition, significantly smaller than the relative root growth rates (45 % and 35 %) of OE1 and OE3, respectively. When the H2O2 concentration was raised to 1.5 mM, the relative root growth rates were 22, 40 and 29 % in the wild-type, OE1 and OE3 seedlings, respectively (Fig. 5a, c, d). These results demonstrated that the transgenic Arabidopsis plants constitutively expressing TaBASS2 had higher tolerance to oxidative stress than the wild-type plants did. Moreover, treatment with methyl viologen (MV), which generates superoxide anions in plastids, demonstrated that these OE lines also displayed enhanced tolerance to plastidial oxidative stress (Additional file 6).Fig. 5

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.