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A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.


TaBASS2 expression in wheat cv. SR3 plants treated with (a) 200 mM NaCl, (b) 100 μM ABA or (c) 10 mM H2O2, and in different tissues (d). Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk in (a-c) indicate significant differences from those at 0 h (P < 0.05, t-test). The expression levels were determined by RT-qPCR using TaCyclophilin as the internal control
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Fig1: TaBASS2 expression in wheat cv. SR3 plants treated with (a) 200 mM NaCl, (b) 100 μM ABA or (c) 10 mM H2O2, and in different tissues (d). Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk in (a-c) indicate significant differences from those at 0 h (P < 0.05, t-test). The expression levels were determined by RT-qPCR using TaCyclophilin as the internal control

Mentions: To determine the expression pattern of TaBASS2 under salinity stress, three-leaf-stage SR3 seedlings were treated with 200 mM NaCl solution and monitored TaBASS2 expression in roots. As shown in Fig. 1a, TaBASS2 was induced by two-fold as early as 1 h of the treatment, and the expression level increased up to four-fold at 24 h. The treatment with H2O2 or ABA also resulted in a similar induction of TaBASS2 expression at 24 h (Fig. 1b, c). These results demonstrated that TaBASS2 expression responded to high level of salinity as well as other stress signals. Various tissue types were also collected for another expression assay. The result showed that TaBASS2 was transcribed in all the tested tissues, with higher expression levels in the green tissues (Fig. 1d).Fig. 1


A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression.

Zhao Y, Ai X, Wang M, Xiao L, Xia G - BMC Plant Biol. (2016)

TaBASS2 expression in wheat cv. SR3 plants treated with (a) 200 mM NaCl, (b) 100 μM ABA or (c) 10 mM H2O2, and in different tissues (d). Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk in (a-c) indicate significant differences from those at 0 h (P < 0.05, t-test). The expression levels were determined by RT-qPCR using TaCyclophilin as the internal control
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862123&req=5

Fig1: TaBASS2 expression in wheat cv. SR3 plants treated with (a) 200 mM NaCl, (b) 100 μM ABA or (c) 10 mM H2O2, and in different tissues (d). Error bars represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk in (a-c) indicate significant differences from those at 0 h (P < 0.05, t-test). The expression levels were determined by RT-qPCR using TaCyclophilin as the internal control
Mentions: To determine the expression pattern of TaBASS2 under salinity stress, three-leaf-stage SR3 seedlings were treated with 200 mM NaCl solution and monitored TaBASS2 expression in roots. As shown in Fig. 1a, TaBASS2 was induced by two-fold as early as 1 h of the treatment, and the expression level increased up to four-fold at 24 h. The treatment with H2O2 or ABA also resulted in a similar induction of TaBASS2 expression at 24 h (Fig. 1b, c). These results demonstrated that TaBASS2 expression responded to high level of salinity as well as other stress signals. Various tissue types were also collected for another expression assay. The result showed that TaBASS2 was transcribed in all the tested tissues, with higher expression levels in the green tissues (Fig. 1d).Fig. 1

Bottom Line: However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression.This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.

ABSTRACT

Background: High salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.

Results: A pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.

Conclusions: Our work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.

No MeSH data available.