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Maternal high fat intake affects the development and transcriptional profile of fetal intestine in late gestation using pig model.

Che L, Liu P, Yang Z, Che L, Hu L, Qin L, Wang R, Fang Z, Lin Y, Xu S, Feng B, Li J, Wu D - Lipids Health Dis (2016)

Bottom Line: At d 90 of gestation, two fetuses each gilt were removed by cesarean section.The results showed that feeding HF diet markedly increased the fetal weight and lactase activity, also tended to increase intestinal morphology.Collectively, it could be concluded that maternal high fat intake was able to increase fetal weight and lactase activity, however, it altered the intestinal immune response, signal transduction and metabolism.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, No.46, Xinkang Road, Ya'an, Sichuan, 625014, People's Republic of China. clianqiang@hotmail.com.

ABSTRACT

Background: The objective of this study was to investigate the effects of maternal high fat intake on intestinal development and transcriptional profile.

Methods: Eight gilts with similar age and body weight were randomly allocated into 2 groups receiving the control and high fat diets (HF diet) from d 30 to 90 of gestation, with 4 gilts each group and one gilt each pen. At d 90 of gestation, two fetuses each gilt were removed by cesarean section. Intestinal samples were collected for analysis of morphology, enzyme activities and transcriptional profile.

Results: The results showed that feeding HF diet markedly increased the fetal weight and lactase activity, also tended to increase intestinal morphology. Porcine Oligo Microarray analysis indicated that feeding HF diet inhibited 64% of genes (39 genes down-regulated while 22 genes up-regulated),which were related to immune response, cancer and metabolism, also markedly modified 33 signal pathways such as antigen processing and presentation, intestinal immune network for IgA production, Jak-STAT and TGF-ß signaling transductions, pathways in colorectal cancer and glycerolipid metabolism.

Conclusion: Collectively, it could be concluded that maternal high fat intake was able to increase fetal weight and lactase activity, however, it altered the intestinal immune response, signal transduction and metabolism.

No MeSH data available.


Related in: MedlinePlus

Signal pathway enrichment analysis of fetal intestine by HF diet relative to CON diet (n = 4 subpools/group). The pathway terms were according to the down-regulated genes for certain biological processes, enriched categories are those identified as significantly enriched after multiple testing. * P < 0.05, ** P < 0.01. The value by horizontal axis resulted from negative value of Log (enrichment test P value, base 10)
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Fig4: Signal pathway enrichment analysis of fetal intestine by HF diet relative to CON diet (n = 4 subpools/group). The pathway terms were according to the down-regulated genes for certain biological processes, enriched categories are those identified as significantly enriched after multiple testing. * P < 0.05, ** P < 0.01. The value by horizontal axis resulted from negative value of Log (enrichment test P value, base 10)

Mentions: The differentially expressed genes were clustered according to their biological process ontology by Gene Ontology (GO) analysis from the SBS analysis system (http://www.shanghaibiotech.com/). A large number of these genes were associated with antigen processing and presentation [i.e. D74, CD8A, SLA-DOB, SLA-DRB1, SLA-DQA, HSPA1L], intestinal immune network for IgA production [i.e. CD40, IL6, TGFβ1], Jak-STAT signaling pathway [i.e. IL6, STAT2 and PIK3R5], TGF-ß signaling pathway [i.e. TGF-β and PIK3R5], pathways in cancer [i.e. LEF1, PIK3R5, NOS2] and glycerolipid metabolism [i.e. GK, PNLIPRP1] et al. (Table 2, Fig. 4).Fig. 4


Maternal high fat intake affects the development and transcriptional profile of fetal intestine in late gestation using pig model.

Che L, Liu P, Yang Z, Che L, Hu L, Qin L, Wang R, Fang Z, Lin Y, Xu S, Feng B, Li J, Wu D - Lipids Health Dis (2016)

Signal pathway enrichment analysis of fetal intestine by HF diet relative to CON diet (n = 4 subpools/group). The pathway terms were according to the down-regulated genes for certain biological processes, enriched categories are those identified as significantly enriched after multiple testing. * P < 0.05, ** P < 0.01. The value by horizontal axis resulted from negative value of Log (enrichment test P value, base 10)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862081&req=5

Fig4: Signal pathway enrichment analysis of fetal intestine by HF diet relative to CON diet (n = 4 subpools/group). The pathway terms were according to the down-regulated genes for certain biological processes, enriched categories are those identified as significantly enriched after multiple testing. * P < 0.05, ** P < 0.01. The value by horizontal axis resulted from negative value of Log (enrichment test P value, base 10)
Mentions: The differentially expressed genes were clustered according to their biological process ontology by Gene Ontology (GO) analysis from the SBS analysis system (http://www.shanghaibiotech.com/). A large number of these genes were associated with antigen processing and presentation [i.e. D74, CD8A, SLA-DOB, SLA-DRB1, SLA-DQA, HSPA1L], intestinal immune network for IgA production [i.e. CD40, IL6, TGFβ1], Jak-STAT signaling pathway [i.e. IL6, STAT2 and PIK3R5], TGF-ß signaling pathway [i.e. TGF-β and PIK3R5], pathways in cancer [i.e. LEF1, PIK3R5, NOS2] and glycerolipid metabolism [i.e. GK, PNLIPRP1] et al. (Table 2, Fig. 4).Fig. 4

Bottom Line: At d 90 of gestation, two fetuses each gilt were removed by cesarean section.The results showed that feeding HF diet markedly increased the fetal weight and lactase activity, also tended to increase intestinal morphology.Collectively, it could be concluded that maternal high fat intake was able to increase fetal weight and lactase activity, however, it altered the intestinal immune response, signal transduction and metabolism.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, No.46, Xinkang Road, Ya'an, Sichuan, 625014, People's Republic of China. clianqiang@hotmail.com.

ABSTRACT

Background: The objective of this study was to investigate the effects of maternal high fat intake on intestinal development and transcriptional profile.

Methods: Eight gilts with similar age and body weight were randomly allocated into 2 groups receiving the control and high fat diets (HF diet) from d 30 to 90 of gestation, with 4 gilts each group and one gilt each pen. At d 90 of gestation, two fetuses each gilt were removed by cesarean section. Intestinal samples were collected for analysis of morphology, enzyme activities and transcriptional profile.

Results: The results showed that feeding HF diet markedly increased the fetal weight and lactase activity, also tended to increase intestinal morphology. Porcine Oligo Microarray analysis indicated that feeding HF diet inhibited 64% of genes (39 genes down-regulated while 22 genes up-regulated),which were related to immune response, cancer and metabolism, also markedly modified 33 signal pathways such as antigen processing and presentation, intestinal immune network for IgA production, Jak-STAT and TGF-ß signaling transductions, pathways in colorectal cancer and glycerolipid metabolism.

Conclusion: Collectively, it could be concluded that maternal high fat intake was able to increase fetal weight and lactase activity, however, it altered the intestinal immune response, signal transduction and metabolism.

No MeSH data available.


Related in: MedlinePlus