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Action of nicotinic acid on the reversion of hypoxic-inflammatory link on 3T3-L1 adipocytes.

Nakamichi R, Miranda EP, Lobo SM, Tristão VR, Dalboni MA, Quinto BM, Batista MC - Lipids Health Dis (2016)

Bottom Line: The results demonstrated an increase in leptin and PAI-1 (plasminogen activator inhibitor-1) expression, while adiponectin production decreased under hypoxia.However, nicotinic acid reversed adipokine modulation under hypoxic conditions, leading to decreased HIF-1 α expression and increased PPARs expression.Our findings suggest that nicotinic acid blunt the inflammatory response resulting from hypoxia by the reduction of HIF-1 α expression and concomitant increase of PPARs α and γ expression in 3T3-L1 adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, Rua Pedro de Toledo 781, Vila Clementino, São Paulo, Brazil. renata.nakamichi@bol.com.br.

ABSTRACT

Background: Hypoxia resulting from adipocyte expansion is considered the basis of the inflammatory milieu observed in Metabolic Syndrome. Nicotinic acid can act on adipocytes interfering on the inflammatory response. In this study, we investigated the role of HIF-1 α (hypoxia-inducible factor -1 alpha) in the inflammatory process induced by hypoxia. The effect of nicotinic acid on the PPARs (peroxisome proliferator-activated receptors) expression during the inflammatory response was assessed over its action under HIF-1 α in 3T3-L1 adipocytes submitted to hypoxia.

Methods: 3T3-L1 adipocytes were pre-treated with nicotinic acid and incubated under hypoxic conditions. The level of adipokines and HIF-1 α were quantified using immunoassays. Adipokine expression was measured using real-time PCR, whereas PPARs and HIF-1 α expression were analyzed by western blot. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) complemented by Bonferroni's test.

Results: The results demonstrated an increase in leptin and PAI-1 (plasminogen activator inhibitor-1) expression, while adiponectin production decreased under hypoxia. In parallel, induction with hypoxia enhanced HIF-1 α expression, despite causing reduced expression of PPAR α and PPAR γ. However, nicotinic acid reversed adipokine modulation under hypoxic conditions, leading to decreased HIF-1 α expression and increased PPARs expression.

Conclusions: Our findings suggest that nicotinic acid blunt the inflammatory response resulting from hypoxia by the reduction of HIF-1 α expression and concomitant increase of PPARs α and γ expression in 3T3-L1 adipocytes.

No MeSH data available.


Related in: MedlinePlus

Dose effects of nicotinic acid on adiponectin secretion. Serial dilutions of nicotinic acid on adiponectin secretion in 3T3-L1 adipocytes; *10 μM, 25 μM, 50 μM groups versus Control group, p < 0.05. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) - n = 6
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Fig1: Dose effects of nicotinic acid on adiponectin secretion. Serial dilutions of nicotinic acid on adiponectin secretion in 3T3-L1 adipocytes; *10 μM, 25 μM, 50 μM groups versus Control group, p < 0.05. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) - n = 6

Mentions: On the 10th day following the beginning of differentiation, the adipocytes were pre-treated with nicotinic acid (10 μmol/L) (Sigma Aldrich, St. Louis, MO, USA) or the vehicle control (non-supplemented DMEM HG medium) for 24 h at 37 ° C in an incubator containing 5 % CO2 and ideal humidity conditions. After the nicotinic acid treatment, the cells were exposed to hypoxia (1 % O2, 94 % N2 and 5 % CO2), sealed and placed at 37 ° C for different periods of time (4, 8 and 12 h). Serial dilutions of nicotinic acid had been previously tested to obtain a working concentration of 10 μmol/L (Fig. 1). Oxygen saturation was performed in all groups using a blood gas analyzer (ABL5 - Radiometer Copenhagen, Holliston, MA, USA) from laboratory of Hospital do Rim (data not shown).Fig. 1


Action of nicotinic acid on the reversion of hypoxic-inflammatory link on 3T3-L1 adipocytes.

Nakamichi R, Miranda EP, Lobo SM, Tristão VR, Dalboni MA, Quinto BM, Batista MC - Lipids Health Dis (2016)

Dose effects of nicotinic acid on adiponectin secretion. Serial dilutions of nicotinic acid on adiponectin secretion in 3T3-L1 adipocytes; *10 μM, 25 μM, 50 μM groups versus Control group, p < 0.05. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) - n = 6
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862071&req=5

Fig1: Dose effects of nicotinic acid on adiponectin secretion. Serial dilutions of nicotinic acid on adiponectin secretion in 3T3-L1 adipocytes; *10 μM, 25 μM, 50 μM groups versus Control group, p < 0.05. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) - n = 6
Mentions: On the 10th day following the beginning of differentiation, the adipocytes were pre-treated with nicotinic acid (10 μmol/L) (Sigma Aldrich, St. Louis, MO, USA) or the vehicle control (non-supplemented DMEM HG medium) for 24 h at 37 ° C in an incubator containing 5 % CO2 and ideal humidity conditions. After the nicotinic acid treatment, the cells were exposed to hypoxia (1 % O2, 94 % N2 and 5 % CO2), sealed and placed at 37 ° C for different periods of time (4, 8 and 12 h). Serial dilutions of nicotinic acid had been previously tested to obtain a working concentration of 10 μmol/L (Fig. 1). Oxygen saturation was performed in all groups using a blood gas analyzer (ABL5 - Radiometer Copenhagen, Holliston, MA, USA) from laboratory of Hospital do Rim (data not shown).Fig. 1

Bottom Line: The results demonstrated an increase in leptin and PAI-1 (plasminogen activator inhibitor-1) expression, while adiponectin production decreased under hypoxia.However, nicotinic acid reversed adipokine modulation under hypoxic conditions, leading to decreased HIF-1 α expression and increased PPARs expression.Our findings suggest that nicotinic acid blunt the inflammatory response resulting from hypoxia by the reduction of HIF-1 α expression and concomitant increase of PPARs α and γ expression in 3T3-L1 adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, Rua Pedro de Toledo 781, Vila Clementino, São Paulo, Brazil. renata.nakamichi@bol.com.br.

ABSTRACT

Background: Hypoxia resulting from adipocyte expansion is considered the basis of the inflammatory milieu observed in Metabolic Syndrome. Nicotinic acid can act on adipocytes interfering on the inflammatory response. In this study, we investigated the role of HIF-1 α (hypoxia-inducible factor -1 alpha) in the inflammatory process induced by hypoxia. The effect of nicotinic acid on the PPARs (peroxisome proliferator-activated receptors) expression during the inflammatory response was assessed over its action under HIF-1 α in 3T3-L1 adipocytes submitted to hypoxia.

Methods: 3T3-L1 adipocytes were pre-treated with nicotinic acid and incubated under hypoxic conditions. The level of adipokines and HIF-1 α were quantified using immunoassays. Adipokine expression was measured using real-time PCR, whereas PPARs and HIF-1 α expression were analyzed by western blot. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) complemented by Bonferroni's test.

Results: The results demonstrated an increase in leptin and PAI-1 (plasminogen activator inhibitor-1) expression, while adiponectin production decreased under hypoxia. In parallel, induction with hypoxia enhanced HIF-1 α expression, despite causing reduced expression of PPAR α and PPAR γ. However, nicotinic acid reversed adipokine modulation under hypoxic conditions, leading to decreased HIF-1 α expression and increased PPARs expression.

Conclusions: Our findings suggest that nicotinic acid blunt the inflammatory response resulting from hypoxia by the reduction of HIF-1 α expression and concomitant increase of PPARs α and γ expression in 3T3-L1 adipocytes.

No MeSH data available.


Related in: MedlinePlus