Limits...
Benchmarking vector arthropod culture: an example using the African malaria mosquito, Anopheles gambiae (Diptera: Culicidae).

Valerio L, Matilda Collins C, Lees RS, Benedict MQ - Malar. J. (2016)

Bottom Line: The observed outcomes described the physiologically constrained limits.The SOP used to produce experimental material was shown to produces high-quality material, relative to the biologically constrained limits.The comparison between all possible phenotypic outcomes, as determined by biological constraints, with those outcomes obtained using a given rearing protocol is termed "benchmarking".

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, University of Perugia, Perugia, Italy.

ABSTRACT

Background: Numerous important characteristics of adult arthropods are related to their size; this is influenced by conditions experienced as immatures. Arthropods cultured in the laboratory for research, or mass-reared for novel control methods, must therefore be of a standard size range and known quality so that results are reproducible.

Methods: A simple two-step technique to assess laboratory culture methods was demonstrated using the mosquito Anopheles gambiae s.s. as a model. First, the ranges of key development outcomes were determined using various diet levels. The observed outcomes described the physiologically constrained limits. Secondly, the same outcomes were measured when using a standard operating procedure (SOP) for comparison with the determined ranges.

Results: The standard method resulted in similar development rates to those of high and medium diets, wing length between those resulting from the high and medium diets, and larval survival exceeding all benchmark diet level values. The SOP used to produce experimental material was shown to produces high-quality material, relative to the biologically constrained limits.

Conclusions: The comparison between all possible phenotypic outcomes, as determined by biological constraints, with those outcomes obtained using a given rearing protocol is termed "benchmarking". A method is here demonstrated which could be easily adapted to other arthropods, to objectively assess important characters obtained, and methods used, during routine culture that may affect outcomes of research.

No MeSH data available.


Related in: MedlinePlus

Wing length of mosquitoes as a function of treatment: three diet levels in Petri dishes and SOP culturing. a Female mosquitoes; b male mosquitoes
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Fig2: Wing length of mosquitoes as a function of treatment: three diet levels in Petri dishes and SOP culturing. a Female mosquitoes; b male mosquitoes

Mentions: There was a significant interaction between diet level and sex (F = 2.7, df = 286,289, p < 0.05) indicating that the effect varied between males and females. Males responded to, but were less responsive to, the high diet than were females (Fig. 2).Fig. 2


Benchmarking vector arthropod culture: an example using the African malaria mosquito, Anopheles gambiae (Diptera: Culicidae).

Valerio L, Matilda Collins C, Lees RS, Benedict MQ - Malar. J. (2016)

Wing length of mosquitoes as a function of treatment: three diet levels in Petri dishes and SOP culturing. a Female mosquitoes; b male mosquitoes
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862070&req=5

Fig2: Wing length of mosquitoes as a function of treatment: three diet levels in Petri dishes and SOP culturing. a Female mosquitoes; b male mosquitoes
Mentions: There was a significant interaction between diet level and sex (F = 2.7, df = 286,289, p < 0.05) indicating that the effect varied between males and females. Males responded to, but were less responsive to, the high diet than were females (Fig. 2).Fig. 2

Bottom Line: The observed outcomes described the physiologically constrained limits.The SOP used to produce experimental material was shown to produces high-quality material, relative to the biologically constrained limits.The comparison between all possible phenotypic outcomes, as determined by biological constraints, with those outcomes obtained using a given rearing protocol is termed "benchmarking".

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, University of Perugia, Perugia, Italy.

ABSTRACT

Background: Numerous important characteristics of adult arthropods are related to their size; this is influenced by conditions experienced as immatures. Arthropods cultured in the laboratory for research, or mass-reared for novel control methods, must therefore be of a standard size range and known quality so that results are reproducible.

Methods: A simple two-step technique to assess laboratory culture methods was demonstrated using the mosquito Anopheles gambiae s.s. as a model. First, the ranges of key development outcomes were determined using various diet levels. The observed outcomes described the physiologically constrained limits. Secondly, the same outcomes were measured when using a standard operating procedure (SOP) for comparison with the determined ranges.

Results: The standard method resulted in similar development rates to those of high and medium diets, wing length between those resulting from the high and medium diets, and larval survival exceeding all benchmark diet level values. The SOP used to produce experimental material was shown to produces high-quality material, relative to the biologically constrained limits.

Conclusions: The comparison between all possible phenotypic outcomes, as determined by biological constraints, with those outcomes obtained using a given rearing protocol is termed "benchmarking". A method is here demonstrated which could be easily adapted to other arthropods, to objectively assess important characters obtained, and methods used, during routine culture that may affect outcomes of research.

No MeSH data available.


Related in: MedlinePlus