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MEK5 overexpression is associated with the occurrence and development of colorectal cancer.

Diao D, Wang L, Wan J, Chen Z, Peng J, Liu H, Chen X, Wang W, Zou L - BMC Cancer (2016)

Bottom Line: Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade.RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangdong, 510120, China. diaodechang@163.com.

ABSTRACT

Background: Mitogen/extracellular signal-regulated kinase kinase-5 (MEK5) has been confirmed to play a pivotal role in tumor carcinogenesis and progression. However, few studies have investigated the role of MEK5 in colorectal cancer (CRC).

Methods: MEK5 expression was determined by immunohistochemistry (IHC) in tissue microarrays (TMAs) containing 2 groups of tissues, and western blotting was used to confirm MEK5 expression in 8 cases of primary CRC tissues and paired normal mucosa. RNA interference was used to verify the biological function of MEK5 gene in the development of CRC.

Results: IHC revealed the expression of MEK5 was higher in tumor tissues (38.1 %), compared with adjacent normal tissue (8.3 %). Western blot showed that, MEK5 expression was upregulated in CRC tumor tissues compared with normal tissue. Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade. Survival analysis revealed that MEK5 overexpression negatively correlated with cancer-free survival (hazard ratio 1.64, P = 0.017). RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.

Conclusion: MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

No MeSH data available.


Related in: MedlinePlus

Survival analysis of primary CRC patients (n = 342). Kaplan-Meier estimates of the DFS (a) and OS (b) according to MEK5 expression in 342 patients. The DFS and OS were significantly lower in patients with MEK5-high expression when compared with patients who had low MEK5 expression. P values were calculated using the log-rank test
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Fig3: Survival analysis of primary CRC patients (n = 342). Kaplan-Meier estimates of the DFS (a) and OS (b) according to MEK5 expression in 342 patients. The DFS and OS were significantly lower in patients with MEK5-high expression when compared with patients who had low MEK5 expression. P values were calculated using the log-rank test

Mentions: The mean patient follow-up time was 71.5 months and the 5-year OS rate of the 342 patients with primary colorectal cancer was 69.6 %, with 102 deaths observed during the follow-up period. The 5-year DFS rate was 67.8 %. During the time of follow-up, 82 patients (24.5 %) developed distant metastasis or local recurrence. According to the univariate analyses, tumor location, TNM stage and differentiation grade were significantly associated with patients’ overall survival and disease-free survival (P < 0.05; Tables 3 and 4). Assessment of CRC patient survival also revealed that overexpression of MEK5 was significantly correlated with short disease-free survival (P < 0.001, Table 3 and Fig. 3-a) and poor overall survival (P = 0.012, Table 4 and Fig. 3-b).Table 3


MEK5 overexpression is associated with the occurrence and development of colorectal cancer.

Diao D, Wang L, Wan J, Chen Z, Peng J, Liu H, Chen X, Wang W, Zou L - BMC Cancer (2016)

Survival analysis of primary CRC patients (n = 342). Kaplan-Meier estimates of the DFS (a) and OS (b) according to MEK5 expression in 342 patients. The DFS and OS were significantly lower in patients with MEK5-high expression when compared with patients who had low MEK5 expression. P values were calculated using the log-rank test
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4862041&req=5

Fig3: Survival analysis of primary CRC patients (n = 342). Kaplan-Meier estimates of the DFS (a) and OS (b) according to MEK5 expression in 342 patients. The DFS and OS were significantly lower in patients with MEK5-high expression when compared with patients who had low MEK5 expression. P values were calculated using the log-rank test
Mentions: The mean patient follow-up time was 71.5 months and the 5-year OS rate of the 342 patients with primary colorectal cancer was 69.6 %, with 102 deaths observed during the follow-up period. The 5-year DFS rate was 67.8 %. During the time of follow-up, 82 patients (24.5 %) developed distant metastasis or local recurrence. According to the univariate analyses, tumor location, TNM stage and differentiation grade were significantly associated with patients’ overall survival and disease-free survival (P < 0.05; Tables 3 and 4). Assessment of CRC patient survival also revealed that overexpression of MEK5 was significantly correlated with short disease-free survival (P < 0.001, Table 3 and Fig. 3-a) and poor overall survival (P = 0.012, Table 4 and Fig. 3-b).Table 3

Bottom Line: Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade.RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangdong, 510120, China. diaodechang@163.com.

ABSTRACT

Background: Mitogen/extracellular signal-regulated kinase kinase-5 (MEK5) has been confirmed to play a pivotal role in tumor carcinogenesis and progression. However, few studies have investigated the role of MEK5 in colorectal cancer (CRC).

Methods: MEK5 expression was determined by immunohistochemistry (IHC) in tissue microarrays (TMAs) containing 2 groups of tissues, and western blotting was used to confirm MEK5 expression in 8 cases of primary CRC tissues and paired normal mucosa. RNA interference was used to verify the biological function of MEK5 gene in the development of CRC.

Results: IHC revealed the expression of MEK5 was higher in tumor tissues (38.1 %), compared with adjacent normal tissue (8.3 %). Western blot showed that, MEK5 expression was upregulated in CRC tumor tissues compared with normal tissue. Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade. Survival analysis revealed that MEK5 overexpression negatively correlated with cancer-free survival (hazard ratio 1.64, P = 0.017). RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.

Conclusion: MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

No MeSH data available.


Related in: MedlinePlus