Limits...
MEK5 overexpression is associated with the occurrence and development of colorectal cancer.

Diao D, Wang L, Wan J, Chen Z, Peng J, Liu H, Chen X, Wang W, Zou L - BMC Cancer (2016)

Bottom Line: Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade.RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangdong, 510120, China. diaodechang@163.com.

ABSTRACT

Background: Mitogen/extracellular signal-regulated kinase kinase-5 (MEK5) has been confirmed to play a pivotal role in tumor carcinogenesis and progression. However, few studies have investigated the role of MEK5 in colorectal cancer (CRC).

Methods: MEK5 expression was determined by immunohistochemistry (IHC) in tissue microarrays (TMAs) containing 2 groups of tissues, and western blotting was used to confirm MEK5 expression in 8 cases of primary CRC tissues and paired normal mucosa. RNA interference was used to verify the biological function of MEK5 gene in the development of CRC.

Results: IHC revealed the expression of MEK5 was higher in tumor tissues (38.1 %), compared with adjacent normal tissue (8.3 %). Western blot showed that, MEK5 expression was upregulated in CRC tumor tissues compared with normal tissue. Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade. Survival analysis revealed that MEK5 overexpression negatively correlated with cancer-free survival (hazard ratio 1.64, P = 0.017). RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.

Conclusion: MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of MEK5 protein expression. Western blot analysis of MEK5 proteins expressed in eight pairs represents colorectal tumor tissues (T) and their matched adjacent non tumor tissues (N). Expression level was normalized with β-actin
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4862041&req=5

Fig2: Western blot analysis of MEK5 protein expression. Western blot analysis of MEK5 proteins expressed in eight pairs represents colorectal tumor tissues (T) and their matched adjacent non tumor tissues (N). Expression level was normalized with β-actin

Mentions: To confirm the expression levels of MEK5 seen by immunostaining in the specimens from our TMA, we examined the expression of MEK5 protein by western blot analysis in 8 randomly selected pairs of CRC tissues and their matched not-tumor colorectal tissues. In 5 of 8 (62.5 %) CRC patients, the total MEK5 protein was up-regulated in tumor tissues compared with their adjacent nontumor colorectal mucosa; furthermore, the ratio of MEK5α to MEKβ was higher in all of the CRC tissues than in their adjacent normal colorectal mucosa (Fig. 2).Fig. 2


MEK5 overexpression is associated with the occurrence and development of colorectal cancer.

Diao D, Wang L, Wan J, Chen Z, Peng J, Liu H, Chen X, Wang W, Zou L - BMC Cancer (2016)

Western blot analysis of MEK5 protein expression. Western blot analysis of MEK5 proteins expressed in eight pairs represents colorectal tumor tissues (T) and their matched adjacent non tumor tissues (N). Expression level was normalized with β-actin
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4862041&req=5

Fig2: Western blot analysis of MEK5 protein expression. Western blot analysis of MEK5 proteins expressed in eight pairs represents colorectal tumor tissues (T) and their matched adjacent non tumor tissues (N). Expression level was normalized with β-actin
Mentions: To confirm the expression levels of MEK5 seen by immunostaining in the specimens from our TMA, we examined the expression of MEK5 protein by western blot analysis in 8 randomly selected pairs of CRC tissues and their matched not-tumor colorectal tissues. In 5 of 8 (62.5 %) CRC patients, the total MEK5 protein was up-regulated in tumor tissues compared with their adjacent nontumor colorectal mucosa; furthermore, the ratio of MEK5α to MEKβ was higher in all of the CRC tissues than in their adjacent normal colorectal mucosa (Fig. 2).Fig. 2

Bottom Line: Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade.RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangdong, 510120, China. diaodechang@163.com.

ABSTRACT

Background: Mitogen/extracellular signal-regulated kinase kinase-5 (MEK5) has been confirmed to play a pivotal role in tumor carcinogenesis and progression. However, few studies have investigated the role of MEK5 in colorectal cancer (CRC).

Methods: MEK5 expression was determined by immunohistochemistry (IHC) in tissue microarrays (TMAs) containing 2 groups of tissues, and western blotting was used to confirm MEK5 expression in 8 cases of primary CRC tissues and paired normal mucosa. RNA interference was used to verify the biological function of MEK5 gene in the development of CRC.

Results: IHC revealed the expression of MEK5 was higher in tumor tissues (38.1 %), compared with adjacent normal tissue (8.3 %). Western blot showed that, MEK5 expression was upregulated in CRC tumor tissues compared with normal tissue. Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade. Survival analysis revealed that MEK5 overexpression negatively correlated with cancer-free survival (hazard ratio 1.64, P = 0.017). RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.

Conclusion: MEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

No MeSH data available.


Related in: MedlinePlus