Transplantation of Photoreceptor Precursors Isolated via a Cell Surface Biomarker Panel From Embryonic Stem Cell-Derived Self-Forming Retina.
Bottom Line: Cell replacement therapy, using pluripotent stem cell-derived photoreceptor cells, may be a feasible future treatment.As genetically labelled cells are not desirable for therapy, here we developed a surface biomarker cell selection strategy for application to complex pluripotent stem cell differentiation cultures.Conversely, unsorted or negatively selected cells do not give rise to newly integrated rods after transplantation.
Affiliation: Stem Cells and Regenerative Medicine Section, UCL Institute of Child Health, University College London, London, United Kingdom.Show MeSH
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Mentions: The distribution of CD markers is usually not restricted to one particular tissue or cell type. We, therefore, confirmed the identity of the PPr biomarker panel‐positive cells generated in the 3D retinal culture system using immuno‐cytochemistry (Fig. 4A, 4B). At day 27, retina were dissociated and plated on coverslips to allow investigation of colocalization of CD73 with Rhodopsin or Recoverin on a single cell basis. Rhodopsin and Recoverin were selected as indicators of photoreceptor cell identity with robust available antibodies. We observed that 36.3% ± 14.4 (n = 3) of the cells labelled with CD73. As expected, the majority of CD73 positive cells were also strongly colabelled with the rod pigment rhodopsin (78.3% ± 10.5; n = 3), confirming the rod photoreceptor identity of PPr biomarker labelled cells generated in the day 27 ESC‐derived retina. Similar analysis conducted using Recoverin showed colabelling of 41% ± 13.6 (n = 3) of the CD73 positive cells.
Affiliation: Stem Cells and Regenerative Medicine Section, UCL Institute of Child Health, University College London, London, United Kingdom.