Transplantation of Photoreceptor Precursors Isolated via a Cell Surface Biomarker Panel From Embryonic Stem Cell-Derived Self-Forming Retina.
Bottom Line: Cell replacement therapy, using pluripotent stem cell-derived photoreceptor cells, may be a feasible future treatment.As genetically labelled cells are not desirable for therapy, here we developed a surface biomarker cell selection strategy for application to complex pluripotent stem cell differentiation cultures.Conversely, unsorted or negatively selected cells do not give rise to newly integrated rods after transplantation.
Affiliation: Stem Cells and Regenerative Medicine Section, UCL Institute of Child Health, University College London, London, United Kingdom.Show MeSH
Related in: MedlinePlus
Mentions: PPrs can be generated efficiently using a previously described embryoid body‐based 3D mouse ESC (mESC) differentiation system 20, 22. In this culture system, continuous neuroepithelia are readily produced within 5 days of differentiation and optic vesicle‐like structures appear around days 7–9 (Fig. 2 shows optic vesicle neuroepithelium at day 12). Retinal cell genesis proceeds in a sequence similar to normal retinal development with all neural retinal cell types being present and correctly organized in layers by day 29 of the differentiation procedure, a stage that has been shown to correlate with P4–P8 during mouse retinal development 22.
Affiliation: Stem Cells and Regenerative Medicine Section, UCL Institute of Child Health, University College London, London, United Kingdom.