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Diagnostic values of vascular endothelial growth factor and epidermal growth factor receptor for benign and malignant hydrothorax.

Gu Y, Zhang M, Li GH, Gao JZ, Guo L, Qiao XJ, Wang LH, He L, Wang ML, Yan L, Fu XH - Chin. Med. J. (2015)

Bottom Line: The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax.VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions.Joint detection can improve the diagnostic sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010059, China.

ABSTRACT

Background: Hydrothorax, as one of the common complications of malignant tumors, still cannot be sensitively detected in clinical practice, thus requiring a sensitive, specific method for diagnosis. The aim of this study was to analyze the correlation between levels of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in patients with benign and malignant hydrothorax.

Methods: The contents of VEGF in the pleural effusion and serum of the patients with malignant pleural effusion (n = 35) and benign pleural effusion (n = 30) were detected by double antibody sandwich enzyme linked immunosorbent assay. The gene copy number level of EGFR in pleural effusion was detected by fluorescence in situ hybridization (FISH). The points with the highest sensitivity and specificity were selected as the critical values to calculate the diagnostic value of the VEGF in pleural effusion and serum, and EGFR gene copy number in pleural effusion.

Results: The contents of VEGF in pleural effusion and serum of patients with malignant hydrothorax were (384.91 ± 120.18), and (129.62 ± 46.35) ng/L, respectively, which were significantly higher than those of the patients with benign hydrothorax (207.97 ± 64.04), (63.49 ± 24.58) ng/L (P < 0.01). The sensitivity and specificity of detecting VEGF in pleural effusion were 80.0% and 96.7% (the boundary value was 297.06 ng/L), respectively for diagnosing benign and malignant hydrothorax. The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax. The diagnostic efficiencies of EGFR and VEGF in hydrothorax were similar. There was a significant correlation between EGFR and VEGF in hydrothorax (P < 0.01).

Conclusions: VEGF and EGFR play important roles in the formation of pleural effusion. VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions. It is feasible to detect the gene copy number of the pleural effusion cell mass EGFR by FISH technique. Joint detection can improve the diagnostic sensitivity.

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Fluorescence in situ hybridization (FISH) was used to detect the gene copy number of epidermal growth factor receptor (EGFR) in malignant pleural effusion (the red signal was EGFR gene amplification and cluster formation; the green signal was the No. 7 chromosome centromere) (original magnification ×200). (a) Negative result showed no EGFR gene amplification; (b and c) FISH positive detection showed EGFR gene cluster amplification; (d) EGFR FISH positive (high polysomy); (e) EGFR FISH positive (gene amplification).
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Figure 1: Fluorescence in situ hybridization (FISH) was used to detect the gene copy number of epidermal growth factor receptor (EGFR) in malignant pleural effusion (the red signal was EGFR gene amplification and cluster formation; the green signal was the No. 7 chromosome centromere) (original magnification ×200). (a) Negative result showed no EGFR gene amplification; (b and c) FISH positive detection showed EGFR gene cluster amplification; (d) EGFR FISH positive (high polysomy); (e) EGFR FISH positive (gene amplification).

Mentions: FISH was used to detect the gene copy number of EGFR in the 30 benign pleural effusion specimens. One of the benign pleural effusion specimens was positive and the remaining specimens were negative. In the malignant pleural effusion, 15 results were negative [Figure 1a] and 20 results were positive [Figure 1b and 1c]. The positive detection rate of malignant hydrothorax was 57.14% (20/35). Among the positive specimens, seven exhibited EGFR gene amplification and 13 exhibited high polysomy of EGFR gene. Among the 16 cases of lung adenocarcinoma, four cases (25.0%) exhibited cluster amplification, 10 cases exhibited punctiform amplification (62.5%), and two cases exhibited no amplification. The adenocarcinoma amplification rate was 87.5%. Among the 14 cases of squamous cell lung carcinoma, three cases exhibited EGFR gene cluster amplification (21.4%) [Figure 1d and 1e], three cases exhibited punctiform amplification (21.4%), and eight cases exhibited no amplification (57.1%). The amplification rate of squamous carcinoma was 42.8%. An exact probability test was used to discuss the relationship between the gene copy number of EGFR in malignant pleural effusion and demographic and clinicopathological characteristics. There was no significant relationship between the detection result and gender, age, or smoking (P > 0.05). However, there was a significant difference between pathological pattern and the detection result, and the positive rate of adenocarcinoma (87.5%) by FISH was higher than that of the squamous carcinoma (42.8%) [P < 0.01, Table 3].


Diagnostic values of vascular endothelial growth factor and epidermal growth factor receptor for benign and malignant hydrothorax.

Gu Y, Zhang M, Li GH, Gao JZ, Guo L, Qiao XJ, Wang LH, He L, Wang ML, Yan L, Fu XH - Chin. Med. J. (2015)

Fluorescence in situ hybridization (FISH) was used to detect the gene copy number of epidermal growth factor receptor (EGFR) in malignant pleural effusion (the red signal was EGFR gene amplification and cluster formation; the green signal was the No. 7 chromosome centromere) (original magnification ×200). (a) Negative result showed no EGFR gene amplification; (b and c) FISH positive detection showed EGFR gene cluster amplification; (d) EGFR FISH positive (high polysomy); (e) EGFR FISH positive (gene amplification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837859&req=5

Figure 1: Fluorescence in situ hybridization (FISH) was used to detect the gene copy number of epidermal growth factor receptor (EGFR) in malignant pleural effusion (the red signal was EGFR gene amplification and cluster formation; the green signal was the No. 7 chromosome centromere) (original magnification ×200). (a) Negative result showed no EGFR gene amplification; (b and c) FISH positive detection showed EGFR gene cluster amplification; (d) EGFR FISH positive (high polysomy); (e) EGFR FISH positive (gene amplification).
Mentions: FISH was used to detect the gene copy number of EGFR in the 30 benign pleural effusion specimens. One of the benign pleural effusion specimens was positive and the remaining specimens were negative. In the malignant pleural effusion, 15 results were negative [Figure 1a] and 20 results were positive [Figure 1b and 1c]. The positive detection rate of malignant hydrothorax was 57.14% (20/35). Among the positive specimens, seven exhibited EGFR gene amplification and 13 exhibited high polysomy of EGFR gene. Among the 16 cases of lung adenocarcinoma, four cases (25.0%) exhibited cluster amplification, 10 cases exhibited punctiform amplification (62.5%), and two cases exhibited no amplification. The adenocarcinoma amplification rate was 87.5%. Among the 14 cases of squamous cell lung carcinoma, three cases exhibited EGFR gene cluster amplification (21.4%) [Figure 1d and 1e], three cases exhibited punctiform amplification (21.4%), and eight cases exhibited no amplification (57.1%). The amplification rate of squamous carcinoma was 42.8%. An exact probability test was used to discuss the relationship between the gene copy number of EGFR in malignant pleural effusion and demographic and clinicopathological characteristics. There was no significant relationship between the detection result and gender, age, or smoking (P > 0.05). However, there was a significant difference between pathological pattern and the detection result, and the positive rate of adenocarcinoma (87.5%) by FISH was higher than that of the squamous carcinoma (42.8%) [P < 0.01, Table 3].

Bottom Line: The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax.VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions.Joint detection can improve the diagnostic sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010059, China.

ABSTRACT

Background: Hydrothorax, as one of the common complications of malignant tumors, still cannot be sensitively detected in clinical practice, thus requiring a sensitive, specific method for diagnosis. The aim of this study was to analyze the correlation between levels of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in patients with benign and malignant hydrothorax.

Methods: The contents of VEGF in the pleural effusion and serum of the patients with malignant pleural effusion (n = 35) and benign pleural effusion (n = 30) were detected by double antibody sandwich enzyme linked immunosorbent assay. The gene copy number level of EGFR in pleural effusion was detected by fluorescence in situ hybridization (FISH). The points with the highest sensitivity and specificity were selected as the critical values to calculate the diagnostic value of the VEGF in pleural effusion and serum, and EGFR gene copy number in pleural effusion.

Results: The contents of VEGF in pleural effusion and serum of patients with malignant hydrothorax were (384.91 ± 120.18), and (129.62 ± 46.35) ng/L, respectively, which were significantly higher than those of the patients with benign hydrothorax (207.97 ± 64.04), (63.49 ± 24.58) ng/L (P < 0.01). The sensitivity and specificity of detecting VEGF in pleural effusion were 80.0% and 96.7% (the boundary value was 297.06 ng/L), respectively for diagnosing benign and malignant hydrothorax. The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax. The diagnostic efficiencies of EGFR and VEGF in hydrothorax were similar. There was a significant correlation between EGFR and VEGF in hydrothorax (P < 0.01).

Conclusions: VEGF and EGFR play important roles in the formation of pleural effusion. VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions. It is feasible to detect the gene copy number of the pleural effusion cell mass EGFR by FISH technique. Joint detection can improve the diagnostic sensitivity.

Show MeSH
Related in: MedlinePlus