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Solid lipid nanoparticles loaded with edaravone for inner ear protection after noise exposure.

Gao G, Liu Y, Zhou CH, Jiang P, Sun JJ - Chin. Med. J. (2015)

Bottom Line: Acoustic stress-induced ROS formation and edaravone exerted a protective effect on the cochlea.Comparisons of hearing thresholds and ROS changes in different animal groups showed that the threshold shift and ROS generation were significantly lower in treated animals than in those without treatment, especially in the edaravone SLN intratympanic injection group.Edaravone SLNs show noticeable slow-release effects and have certain protective effects against noise-induced hearing loss (NIHL).

View Article: PubMed Central - PubMed

Affiliation: Center for Otolaryngology of the People's Liberation Army, Naval General Hospital, Beijing 100048, China.

ABSTRACT

Background: Antioxidants and the duration of treatment after noise exposure on hearing recovery are important. We investigated the protective effects of an antioxidant substance, edaravone, and its slow-release dosage form, edaravone solid lipid nanoparticles (SLNs), in steady noise-exposed guinea pigs.

Methods: SLNs loaded with edaravone were produced by an ultrasound technique. Edaravone solution or edaravone SLNs were administered by intratympanic or intravenous injection after the 1 st day of noise exposure. Guinea pigs were exposed to 110 dB sound pressure level (SPL) noise, centered at 0.25-4.0 kHz, for 4 days at 2 h/d. After noise exposure, the guinea pigs underwent auditory brainstem response (ABR) threshold measurements, reactive oxygen species (ROS) were detected in their cochleas with electron spin resonance (ESR), and outer hair cells (OHCs) were counted with silvernitrate (AgNO 3 ) staining at 1, 4, and 6 days.

Results: The ultrasound technique was able to prepare adequate edaravone SLNs with a mean particle size of 93.6 nm and entrapment efficiency of 76.7%. Acoustic stress-induced ROS formation and edaravone exerted a protective effect on the cochlea. Comparisons of hearing thresholds and ROS changes in different animal groups showed that the threshold shift and ROS generation were significantly lower in treated animals than in those without treatment, especially in the edaravone SLN intratympanic injection group.

Conclusions: Edaravone SLNs show noticeable slow-release effects and have certain protective effects against noise-induced hearing loss (NIHL).

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Related in: MedlinePlus

Changes in hair cell loss by AgNO3 staining in guinea pigs. By counting hair cells, we found that the percentages of outer hair cell (OHC) loss showed no difference among the groups.
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Figure 5: Changes in hair cell loss by AgNO3 staining in guinea pigs. By counting hair cells, we found that the percentages of outer hair cell (OHC) loss showed no difference among the groups.

Mentions: Missing hair cells were observed and counted with AgNO3 staining [Figure 4], and the percentages of OHC loss were evaluated as the mean loss for each treatment group [Figure 5]. From the pictures, we can see the inner hair cells (IHC, ed group from the noise exposure group. The ROS level of the intravenous injected edaravone solu↗). But the percentages of OHC loss showed no difference among the groups.


Solid lipid nanoparticles loaded with edaravone for inner ear protection after noise exposure.

Gao G, Liu Y, Zhou CH, Jiang P, Sun JJ - Chin. Med. J. (2015)

Changes in hair cell loss by AgNO3 staining in guinea pigs. By counting hair cells, we found that the percentages of outer hair cell (OHC) loss showed no difference among the groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837839&req=5

Figure 5: Changes in hair cell loss by AgNO3 staining in guinea pigs. By counting hair cells, we found that the percentages of outer hair cell (OHC) loss showed no difference among the groups.
Mentions: Missing hair cells were observed and counted with AgNO3 staining [Figure 4], and the percentages of OHC loss were evaluated as the mean loss for each treatment group [Figure 5]. From the pictures, we can see the inner hair cells (IHC, ed group from the noise exposure group. The ROS level of the intravenous injected edaravone solu↗). But the percentages of OHC loss showed no difference among the groups.

Bottom Line: Acoustic stress-induced ROS formation and edaravone exerted a protective effect on the cochlea.Comparisons of hearing thresholds and ROS changes in different animal groups showed that the threshold shift and ROS generation were significantly lower in treated animals than in those without treatment, especially in the edaravone SLN intratympanic injection group.Edaravone SLNs show noticeable slow-release effects and have certain protective effects against noise-induced hearing loss (NIHL).

View Article: PubMed Central - PubMed

Affiliation: Center for Otolaryngology of the People's Liberation Army, Naval General Hospital, Beijing 100048, China.

ABSTRACT

Background: Antioxidants and the duration of treatment after noise exposure on hearing recovery are important. We investigated the protective effects of an antioxidant substance, edaravone, and its slow-release dosage form, edaravone solid lipid nanoparticles (SLNs), in steady noise-exposed guinea pigs.

Methods: SLNs loaded with edaravone were produced by an ultrasound technique. Edaravone solution or edaravone SLNs were administered by intratympanic or intravenous injection after the 1 st day of noise exposure. Guinea pigs were exposed to 110 dB sound pressure level (SPL) noise, centered at 0.25-4.0 kHz, for 4 days at 2 h/d. After noise exposure, the guinea pigs underwent auditory brainstem response (ABR) threshold measurements, reactive oxygen species (ROS) were detected in their cochleas with electron spin resonance (ESR), and outer hair cells (OHCs) were counted with silvernitrate (AgNO 3 ) staining at 1, 4, and 6 days.

Results: The ultrasound technique was able to prepare adequate edaravone SLNs with a mean particle size of 93.6 nm and entrapment efficiency of 76.7%. Acoustic stress-induced ROS formation and edaravone exerted a protective effect on the cochlea. Comparisons of hearing thresholds and ROS changes in different animal groups showed that the threshold shift and ROS generation were significantly lower in treated animals than in those without treatment, especially in the edaravone SLN intratympanic injection group.

Conclusions: Edaravone SLNs show noticeable slow-release effects and have certain protective effects against noise-induced hearing loss (NIHL).

Show MeSH
Related in: MedlinePlus