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Prognostic significance of tumor-infiltrating CD8⁺ or CD3⁺ T lymphocytes and interleukin-2 expression in radically resected non-small cell lung cancer.

Tian C, Lu S, Fan Q, Zhang W, Jiao S, Zhao X, Wu Z, Sun L, Wang L - Chin. Med. J. (2015)

Bottom Line: Altered immunoresponse is associated with tumorigenesis and cancer progression.Clinicopathological and survival data were collected and analyzed using the Chi-squared test, Kaplan-Meier curves, and the log-rank test or the Cox regression model.In addition, the levels of CD8 + T lymphocytes were associated with an unfavorable 5-year OS, whereas patients with high levels of CD3 + T lymphocytes in tumor lesions and IL-2-expressing tumors had significantly better 5-year OS rates than patients with low levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China.

ABSTRACT

Background: Altered immunoresponse is associated with tumorigenesis and cancer progression. This study assessed the levels of tumor-infiltrating CD3 + or CD8 + T lymphocytes and interleukin-2 (IL-2) protein in radically resected non-small cell lung cancer (NSCLC) tissues to predict overall survival (OS) of the patients.

Methods: Paraffin-embedded tissue specimens from 129 NSCLC patients were retrospectively collected for immunostaining of CD8 + , CD3 + , and IL-2 expression. Clinicopathological and survival data were collected and analyzed using the Chi-squared test, Kaplan-Meier curves, and the log-rank test or the Cox regression model.

Results: The data showed a significant inverse association between CD8 + T lymphocyte levels and IL-2 expression (r = -0.927; P = 0.000) and between the levels of CD8 + and CD3 + T lymphocytes (r = -0.722; P = 0.000), but a positive association between CD3 + T lymphocyte levels and IL-2 expression (r = 0.781; P = 0.000) in NSCLC tissues. Furthermore, the levels of CD3 + and CD8 + T lymphocytes and IL-2 expression were associated with tumor stage (P = 0.023, 0.006, and 0.031, respectively) and the level of CD8 + T lymphocytes was associated with the patient gender (P = 0.024). In addition, the levels of CD8 + T lymphocytes were associated with an unfavorable 5-year OS, whereas patients with high levels of CD3 + T lymphocytes in tumor lesions and IL-2-expressing tumors had significantly better 5-year OS rates than patients with low levels.

Conclusions: The levels of CD8 + T cells in tumor lesions and IL-2 expression were both independent predictors of OS for these NSCLC patients. Thus, the detection of tumor-infiltrating CD3 + or CD8 + T lymphocytes and IL-2 expression could be useful to predict the prognosis of radically resected NSCLC patients.

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Representative images of immunohistochemically stained non-small cell lung cancer tissue specimens. (a) High levels of CD3+ T cell infiltration in the tumor stroma (adenocarcinoma [AC]). (b) Low level of CD3+ T cell infiltration in the cancer cell nest and stroma (AC). (c) High level of interleukin-2 (IL-2) expression in the cancer nest (squamous cell carcinoma [SCC]). (d) Low level of IL-2 expression in the cancer nest (SCC). (e) High level of CD8+ T cell infiltration within the cancer stroma (AC). (f) Low level of CD8+ T cell infiltration within the cancer stroma (AC) (×200).
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Figure 1: Representative images of immunohistochemically stained non-small cell lung cancer tissue specimens. (a) High levels of CD3+ T cell infiltration in the tumor stroma (adenocarcinoma [AC]). (b) Low level of CD3+ T cell infiltration in the cancer cell nest and stroma (AC). (c) High level of interleukin-2 (IL-2) expression in the cancer nest (squamous cell carcinoma [SCC]). (d) Low level of IL-2 expression in the cancer nest (SCC). (e) High level of CD8+ T cell infiltration within the cancer stroma (AC). (f) Low level of CD8+ T cell infiltration within the cancer stroma (AC) (×200).

Mentions: Interleukin-2 protein and CD3+ and CD8+ T cells were present in the cancer stroma and cancer cell nests [Figure 1]. Specifically, 91 of these 129 tumor tissue specimens had CD8+ T cells in the cancer nests, with a mean number of 4.65 ± 4.25 CD8+ T cells; while 126 of these 129 specimens showed CD8+ T cells in the cancer stromal tissues, with a mean number of 57.63 ± 23.71. Moreover, 88 of these 129 cases had CD3+ T cells in the cancer nests, with a mean number of 4.95 ± 10.46 CD3+ T cells; and 117 of these 129 cases also showed CD3+ T cells in the cancer stromal tissues, with a mean number of 23.06 ± 21.38. In addition, IL-2 protein was detected in the cancer cells of 122 NSCLC cases and the cancer stromal cells of 79 cases, with mean numbers of 26.08 ± 21.00 and 2.00 ± 2.04, respectively.


Prognostic significance of tumor-infiltrating CD8⁺ or CD3⁺ T lymphocytes and interleukin-2 expression in radically resected non-small cell lung cancer.

Tian C, Lu S, Fan Q, Zhang W, Jiao S, Zhao X, Wu Z, Sun L, Wang L - Chin. Med. J. (2015)

Representative images of immunohistochemically stained non-small cell lung cancer tissue specimens. (a) High levels of CD3+ T cell infiltration in the tumor stroma (adenocarcinoma [AC]). (b) Low level of CD3+ T cell infiltration in the cancer cell nest and stroma (AC). (c) High level of interleukin-2 (IL-2) expression in the cancer nest (squamous cell carcinoma [SCC]). (d) Low level of IL-2 expression in the cancer nest (SCC). (e) High level of CD8+ T cell infiltration within the cancer stroma (AC). (f) Low level of CD8+ T cell infiltration within the cancer stroma (AC) (×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837804&req=5

Figure 1: Representative images of immunohistochemically stained non-small cell lung cancer tissue specimens. (a) High levels of CD3+ T cell infiltration in the tumor stroma (adenocarcinoma [AC]). (b) Low level of CD3+ T cell infiltration in the cancer cell nest and stroma (AC). (c) High level of interleukin-2 (IL-2) expression in the cancer nest (squamous cell carcinoma [SCC]). (d) Low level of IL-2 expression in the cancer nest (SCC). (e) High level of CD8+ T cell infiltration within the cancer stroma (AC). (f) Low level of CD8+ T cell infiltration within the cancer stroma (AC) (×200).
Mentions: Interleukin-2 protein and CD3+ and CD8+ T cells were present in the cancer stroma and cancer cell nests [Figure 1]. Specifically, 91 of these 129 tumor tissue specimens had CD8+ T cells in the cancer nests, with a mean number of 4.65 ± 4.25 CD8+ T cells; while 126 of these 129 specimens showed CD8+ T cells in the cancer stromal tissues, with a mean number of 57.63 ± 23.71. Moreover, 88 of these 129 cases had CD3+ T cells in the cancer nests, with a mean number of 4.95 ± 10.46 CD3+ T cells; and 117 of these 129 cases also showed CD3+ T cells in the cancer stromal tissues, with a mean number of 23.06 ± 21.38. In addition, IL-2 protein was detected in the cancer cells of 122 NSCLC cases and the cancer stromal cells of 79 cases, with mean numbers of 26.08 ± 21.00 and 2.00 ± 2.04, respectively.

Bottom Line: Altered immunoresponse is associated with tumorigenesis and cancer progression.Clinicopathological and survival data were collected and analyzed using the Chi-squared test, Kaplan-Meier curves, and the log-rank test or the Cox regression model.In addition, the levels of CD8 + T lymphocytes were associated with an unfavorable 5-year OS, whereas patients with high levels of CD3 + T lymphocytes in tumor lesions and IL-2-expressing tumors had significantly better 5-year OS rates than patients with low levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China.

ABSTRACT

Background: Altered immunoresponse is associated with tumorigenesis and cancer progression. This study assessed the levels of tumor-infiltrating CD3 + or CD8 + T lymphocytes and interleukin-2 (IL-2) protein in radically resected non-small cell lung cancer (NSCLC) tissues to predict overall survival (OS) of the patients.

Methods: Paraffin-embedded tissue specimens from 129 NSCLC patients were retrospectively collected for immunostaining of CD8 + , CD3 + , and IL-2 expression. Clinicopathological and survival data were collected and analyzed using the Chi-squared test, Kaplan-Meier curves, and the log-rank test or the Cox regression model.

Results: The data showed a significant inverse association between CD8 + T lymphocyte levels and IL-2 expression (r = -0.927; P = 0.000) and between the levels of CD8 + and CD3 + T lymphocytes (r = -0.722; P = 0.000), but a positive association between CD3 + T lymphocyte levels and IL-2 expression (r = 0.781; P = 0.000) in NSCLC tissues. Furthermore, the levels of CD3 + and CD8 + T lymphocytes and IL-2 expression were associated with tumor stage (P = 0.023, 0.006, and 0.031, respectively) and the level of CD8 + T lymphocytes was associated with the patient gender (P = 0.024). In addition, the levels of CD8 + T lymphocytes were associated with an unfavorable 5-year OS, whereas patients with high levels of CD3 + T lymphocytes in tumor lesions and IL-2-expressing tumors had significantly better 5-year OS rates than patients with low levels.

Conclusions: The levels of CD8 + T cells in tumor lesions and IL-2 expression were both independent predictors of OS for these NSCLC patients. Thus, the detection of tumor-infiltrating CD3 + or CD8 + T lymphocytes and IL-2 expression could be useful to predict the prognosis of radically resected NSCLC patients.

Show MeSH
Related in: MedlinePlus