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Pathology interface for the molecular analysis of tissue by mass spectrometry.

Norris JL, Tsui T, Gutierrez DB, Caprioli RM - J Pathol Inform (2016)

Bottom Line: Imaging mass spectrometry (IMS) generates molecular images directly from tissue sections to provide better diagnostic insights and expand the capabilities of clinical anatomic pathology.We describe a microscopy-driven approach to tissue analysis by IMS.The Pathology Interface for Mass Spectrometry is designed to provide clinical access to IMS technology and deliver enhanced diagnostic value.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, National Research Resource for Imaging Mass Spectrometry, Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37240, USA.

ABSTRACT

Background: Imaging mass spectrometry (IMS) generates molecular images directly from tissue sections to provide better diagnostic insights and expand the capabilities of clinical anatomic pathology. Although IMS technology has matured over recent years, the link between microscopy imaging currently used by pathologists and MS-based molecular imaging has not been established.

Methods: We adapted the Vanderbilt University Tissue Core workflow for IMS into a web-based system that facilitates remote collaboration. The platform was designed to perform within acceptable web response times for viewing, annotating, and processing high resolution microscopy images.

Results: We describe a microscopy-driven approach to tissue analysis by IMS.

Conclusion: The Pathology Interface for Mass Spectrometry is designed to provide clinical access to IMS technology and deliver enhanced diagnostic value.

No MeSH data available.


Pathology interface for mass spectrometry transforms the current IMS approach into one practical for the clinical setting, integrating microscopy, and IMS analyses to deliver molecularly specific, clinically valuable results
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Figure 1: Pathology interface for mass spectrometry transforms the current IMS approach into one practical for the clinical setting, integrating microscopy, and IMS analyses to deliver molecularly specific, clinically valuable results

Mentions: We have developed a novel workflow automation web-based software tool – the pathology interface for MS (PIMS) – that overcomes these challenges and facilitates collaboration between anatomical pathologists and analytical scientists through seamless integration of microscopy, sample preparation, and MS analysis [Figure 1]. Importantly, the interface allows biologists and pathologists who are untrained in IMS technology to control the acquisition of MS data. Image annotation performed by the pathologist drives histology-directed sample preparation and IMS analysis,[101112] making this technology easy to use and ultimately accessible to physicians worldwide.


Pathology interface for the molecular analysis of tissue by mass spectrometry.

Norris JL, Tsui T, Gutierrez DB, Caprioli RM - J Pathol Inform (2016)

Pathology interface for mass spectrometry transforms the current IMS approach into one practical for the clinical setting, integrating microscopy, and IMS analyses to deliver molecularly specific, clinically valuable results
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837791&req=5

Figure 1: Pathology interface for mass spectrometry transforms the current IMS approach into one practical for the clinical setting, integrating microscopy, and IMS analyses to deliver molecularly specific, clinically valuable results
Mentions: We have developed a novel workflow automation web-based software tool – the pathology interface for MS (PIMS) – that overcomes these challenges and facilitates collaboration between anatomical pathologists and analytical scientists through seamless integration of microscopy, sample preparation, and MS analysis [Figure 1]. Importantly, the interface allows biologists and pathologists who are untrained in IMS technology to control the acquisition of MS data. Image annotation performed by the pathologist drives histology-directed sample preparation and IMS analysis,[101112] making this technology easy to use and ultimately accessible to physicians worldwide.

Bottom Line: Imaging mass spectrometry (IMS) generates molecular images directly from tissue sections to provide better diagnostic insights and expand the capabilities of clinical anatomic pathology.We describe a microscopy-driven approach to tissue analysis by IMS.The Pathology Interface for Mass Spectrometry is designed to provide clinical access to IMS technology and deliver enhanced diagnostic value.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, National Research Resource for Imaging Mass Spectrometry, Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37240, USA.

ABSTRACT

Background: Imaging mass spectrometry (IMS) generates molecular images directly from tissue sections to provide better diagnostic insights and expand the capabilities of clinical anatomic pathology. Although IMS technology has matured over recent years, the link between microscopy imaging currently used by pathologists and MS-based molecular imaging has not been established.

Methods: We adapted the Vanderbilt University Tissue Core workflow for IMS into a web-based system that facilitates remote collaboration. The platform was designed to perform within acceptable web response times for viewing, annotating, and processing high resolution microscopy images.

Results: We describe a microscopy-driven approach to tissue analysis by IMS.

Conclusion: The Pathology Interface for Mass Spectrometry is designed to provide clinical access to IMS technology and deliver enhanced diagnostic value.

No MeSH data available.