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Reduction of intraarticular adhesion of knee by local application of rapamycin in rabbits via inhibition of fibroblast proliferation and collagen synthesis.

Zhao S, Sun Y, Li X, Wang J, Yan L, Chen H, Wang D, Dai J, He J - J Orthop Surg Res (2016)

Bottom Line: The results shown that RAPA could significantly inhibit the proliferation of fibroblasts and reduce collagen synthesis; in the rabbit model of knee surgery, there were weak scar tissues around the decorticated areas in the 0.2 mg/ml RAPA group; moderate scar tissues were found in the 0.1 mg/ml RAPA group.However, severe fibrous adhesions were found in the 0.05 mg/ml RAPA group and the control group.The hydroxyproline content and the fibroblast density in the 0.2 mg/ml and 0.1 mg/ml RAPA groups were significantly less than those of the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Xiangya Second Hospital, Central South University, Changsha, Hunan, 410012, China.

ABSTRACT

Background: The formation of intraarticular adhesion is a common complication after total knee arthroplasty or anterior cruciate ligament reconstruction. Previously, little research was reported regarding whether the local application of rapamycin (RAPA) could reduce intraarticular adhesion following knee surgery. In our present study, we determined the therapeutic effect of RAPA by local application on the reduction of intraarticular adhesion following knee surgery in rabbits.

Methods: In this study, we built the model of knee surgery according to a previous study. The decorticated areas of the cortical bone were exposed and covered with cotton pads soaked with different concentrations of RAPA or physiological saline for 10 min. All of the rabbits were euthanized 4 weeks after the surgery. Macroscopic evaluation of the hydroxyproline content, the histological morphological analysis and collagen density and fibroblast density were used to evaluate the effect of RAPA on reducing intraarticular adhesion.

Results: The results shown that RAPA could significantly inhibit the proliferation of fibroblasts and reduce collagen synthesis; in the rabbit model of knee surgery, there were weak scar tissues around the decorticated areas in the 0.2 mg/ml RAPA group; moderate scar tissues were found in the 0.1 mg/ml RAPA group. However, severe fibrous adhesions were found in the 0.05 mg/ml RAPA group and the control group. The hydroxyproline content and the fibroblast density in the 0.2 mg/ml and 0.1 mg/ml RAPA groups were significantly less than those of the control group.

Conclusions: We concluded that the local application of RAPA could reduce intraarticular adhesion after knee surgery in the rabbit model; this effect was mediated by inhibition of fibroblast proliferation and collagen synthesis, which may provide a new method for reducing intraarticular adhesion after clinical knee surgery.

No MeSH data available.


Related in: MedlinePlus

Hydroxyproline content in intraarticular scar tissue in the RAPA-treated groups and the control group. The hydroxyproline content is expressed as micrograms per milligram (μg/mg). *P < 0.05, compared with control group; #P < 0.05, and the 0.2 mg/ml RAPA group compared with the other RAPA groups
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Fig1: Hydroxyproline content in intraarticular scar tissue in the RAPA-treated groups and the control group. The hydroxyproline content is expressed as micrograms per milligram (μg/mg). *P < 0.05, compared with control group; #P < 0.05, and the 0.2 mg/ml RAPA group compared with the other RAPA groups

Mentions: The statistical analysis of the hydroxyproline content in the intraarticular scar tissue for each group is shown in Fig. 1. The hydroxyproline content in the 0.2 mg/ml RAPA group was 23.94 ± 1.84 μg/mg, which was significantly less than those in the 0.1 mg/ml RAPA group (33.75 ± 4.31 μg/mg, P = 0.022), the 0.05 mg/ml RAPA group (44.50 ± 4.35 μg/mg, P = 0.002) and the control group (48.37 ± 4.43 μg/mg, P = 0.001). The content in the 0.1 mg/ml RAPA group was also less than those in the 0.05 mg/ml RAPA group (P = 0.039) and the control group (P = 0.015). However, the content in the 0.05 mg/ml RAPA group showed no significant difference compared with those of the control group (P = 0.341).Fig. 1


Reduction of intraarticular adhesion of knee by local application of rapamycin in rabbits via inhibition of fibroblast proliferation and collagen synthesis.

Zhao S, Sun Y, Li X, Wang J, Yan L, Chen H, Wang D, Dai J, He J - J Orthop Surg Res (2016)

Hydroxyproline content in intraarticular scar tissue in the RAPA-treated groups and the control group. The hydroxyproline content is expressed as micrograms per milligram (μg/mg). *P < 0.05, compared with control group; #P < 0.05, and the 0.2 mg/ml RAPA group compared with the other RAPA groups
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837638&req=5

Fig1: Hydroxyproline content in intraarticular scar tissue in the RAPA-treated groups and the control group. The hydroxyproline content is expressed as micrograms per milligram (μg/mg). *P < 0.05, compared with control group; #P < 0.05, and the 0.2 mg/ml RAPA group compared with the other RAPA groups
Mentions: The statistical analysis of the hydroxyproline content in the intraarticular scar tissue for each group is shown in Fig. 1. The hydroxyproline content in the 0.2 mg/ml RAPA group was 23.94 ± 1.84 μg/mg, which was significantly less than those in the 0.1 mg/ml RAPA group (33.75 ± 4.31 μg/mg, P = 0.022), the 0.05 mg/ml RAPA group (44.50 ± 4.35 μg/mg, P = 0.002) and the control group (48.37 ± 4.43 μg/mg, P = 0.001). The content in the 0.1 mg/ml RAPA group was also less than those in the 0.05 mg/ml RAPA group (P = 0.039) and the control group (P = 0.015). However, the content in the 0.05 mg/ml RAPA group showed no significant difference compared with those of the control group (P = 0.341).Fig. 1

Bottom Line: The results shown that RAPA could significantly inhibit the proliferation of fibroblasts and reduce collagen synthesis; in the rabbit model of knee surgery, there were weak scar tissues around the decorticated areas in the 0.2 mg/ml RAPA group; moderate scar tissues were found in the 0.1 mg/ml RAPA group.However, severe fibrous adhesions were found in the 0.05 mg/ml RAPA group and the control group.The hydroxyproline content and the fibroblast density in the 0.2 mg/ml and 0.1 mg/ml RAPA groups were significantly less than those of the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Xiangya Second Hospital, Central South University, Changsha, Hunan, 410012, China.

ABSTRACT

Background: The formation of intraarticular adhesion is a common complication after total knee arthroplasty or anterior cruciate ligament reconstruction. Previously, little research was reported regarding whether the local application of rapamycin (RAPA) could reduce intraarticular adhesion following knee surgery. In our present study, we determined the therapeutic effect of RAPA by local application on the reduction of intraarticular adhesion following knee surgery in rabbits.

Methods: In this study, we built the model of knee surgery according to a previous study. The decorticated areas of the cortical bone were exposed and covered with cotton pads soaked with different concentrations of RAPA or physiological saline for 10 min. All of the rabbits were euthanized 4 weeks after the surgery. Macroscopic evaluation of the hydroxyproline content, the histological morphological analysis and collagen density and fibroblast density were used to evaluate the effect of RAPA on reducing intraarticular adhesion.

Results: The results shown that RAPA could significantly inhibit the proliferation of fibroblasts and reduce collagen synthesis; in the rabbit model of knee surgery, there were weak scar tissues around the decorticated areas in the 0.2 mg/ml RAPA group; moderate scar tissues were found in the 0.1 mg/ml RAPA group. However, severe fibrous adhesions were found in the 0.05 mg/ml RAPA group and the control group. The hydroxyproline content and the fibroblast density in the 0.2 mg/ml and 0.1 mg/ml RAPA groups were significantly less than those of the control group.

Conclusions: We concluded that the local application of RAPA could reduce intraarticular adhesion after knee surgery in the rabbit model; this effect was mediated by inhibition of fibroblast proliferation and collagen synthesis, which may provide a new method for reducing intraarticular adhesion after clinical knee surgery.

No MeSH data available.


Related in: MedlinePlus