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Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).

Matějů J, Chanová M, Modrý D, Mitková B, Hrazdilová K, Žampachová V, Kolářová L - BMC Infect. Dis. (2016)

Bottom Line: In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans.At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad).The findings confirm the natural setting of D. repens in South Moravian Region of the CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.

ABSTRACT

Background: Human dirofilariasis is a zoonotic infection that continues to spread to previously unaffected areas of Europe. In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans. In the present paper, human Dirofilaria infections are reported from the country for the first time.

Case presentation: The samples from five patients with suspected tissue helminthiases were investigated. In particular cases, nematodes were isolated from various tissues including skin of lower leg, soft tissues of finger, subcutaneous tissue of hypogastrium, lymph node and peritoneum. The diagnosis was based on light microscopic morphology and/or DNA analysis of the worms. In addition, ELISA examination of patients' sera for anti-filaria IgG antibodies was performed.

Conclusions: In the CR, five cases of human dirofilariasis caused by Dirofilaria repens were recorded during 2010-2014 (species determination for three of them was confirmed besides morphological also by DNA analysis). At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad). The findings confirm the natural setting of D. repens in South Moravian Region of the CR. Dirofilariasis should be therefore considered as endemic in this area where it may represent a significant risk factor for public health.

No MeSH data available.


Related in: MedlinePlus

The worm extracted from the nodule on the left middle-finger of the patient (Case 4). a. Detail on cuticular structure with Dirofilaria-specific longitudinal ridges (-----) and transversal striation (∙∙∙∙∙); Photo P. Kotíková
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Fig3: The worm extracted from the nodule on the left middle-finger of the patient (Case 4). a. Detail on cuticular structure with Dirofilaria-specific longitudinal ridges (-----) and transversal striation (∙∙∙∙∙); Photo P. Kotíková

Mentions: A roundworm with longitudinal (sporadically branching) ridges and finer transversal stripes on the cuticle of entire body except anterior end was identified. Based on the morphology, the object was determined as Dirofilaria spp. (suspected D. repens; Fig. 3). The DNA analysis confirmed D. repens. Serological examination of the serum sample taken 1 day after intervention did not reveal anti-filaria IgG antibodies.Fig. 3


Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).

Matějů J, Chanová M, Modrý D, Mitková B, Hrazdilová K, Žampachová V, Kolářová L - BMC Infect. Dis. (2016)

The worm extracted from the nodule on the left middle-finger of the patient (Case 4). a. Detail on cuticular structure with Dirofilaria-specific longitudinal ridges (-----) and transversal striation (∙∙∙∙∙); Photo P. Kotíková
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837637&req=5

Fig3: The worm extracted from the nodule on the left middle-finger of the patient (Case 4). a. Detail on cuticular structure with Dirofilaria-specific longitudinal ridges (-----) and transversal striation (∙∙∙∙∙); Photo P. Kotíková
Mentions: A roundworm with longitudinal (sporadically branching) ridges and finer transversal stripes on the cuticle of entire body except anterior end was identified. Based on the morphology, the object was determined as Dirofilaria spp. (suspected D. repens; Fig. 3). The DNA analysis confirmed D. repens. Serological examination of the serum sample taken 1 day after intervention did not reveal anti-filaria IgG antibodies.Fig. 3

Bottom Line: In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans.At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad).The findings confirm the natural setting of D. repens in South Moravian Region of the CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.

ABSTRACT

Background: Human dirofilariasis is a zoonotic infection that continues to spread to previously unaffected areas of Europe. In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans. In the present paper, human Dirofilaria infections are reported from the country for the first time.

Case presentation: The samples from five patients with suspected tissue helminthiases were investigated. In particular cases, nematodes were isolated from various tissues including skin of lower leg, soft tissues of finger, subcutaneous tissue of hypogastrium, lymph node and peritoneum. The diagnosis was based on light microscopic morphology and/or DNA analysis of the worms. In addition, ELISA examination of patients' sera for anti-filaria IgG antibodies was performed.

Conclusions: In the CR, five cases of human dirofilariasis caused by Dirofilaria repens were recorded during 2010-2014 (species determination for three of them was confirmed besides morphological also by DNA analysis). At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad). The findings confirm the natural setting of D. repens in South Moravian Region of the CR. Dirofilariasis should be therefore considered as endemic in this area where it may represent a significant risk factor for public health.

No MeSH data available.


Related in: MedlinePlus