Limits...
Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).

Matějů J, Chanová M, Modrý D, Mitková B, Hrazdilová K, Žampachová V, Kolářová L - BMC Infect. Dis. (2016)

Bottom Line: In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans.At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad).The findings confirm the natural setting of D. repens in South Moravian Region of the CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.

ABSTRACT

Background: Human dirofilariasis is a zoonotic infection that continues to spread to previously unaffected areas of Europe. In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans. In the present paper, human Dirofilaria infections are reported from the country for the first time.

Case presentation: The samples from five patients with suspected tissue helminthiases were investigated. In particular cases, nematodes were isolated from various tissues including skin of lower leg, soft tissues of finger, subcutaneous tissue of hypogastrium, lymph node and peritoneum. The diagnosis was based on light microscopic morphology and/or DNA analysis of the worms. In addition, ELISA examination of patients' sera for anti-filaria IgG antibodies was performed.

Conclusions: In the CR, five cases of human dirofilariasis caused by Dirofilaria repens were recorded during 2010-2014 (species determination for three of them was confirmed besides morphological also by DNA analysis). At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad). The findings confirm the natural setting of D. repens in South Moravian Region of the CR. Dirofilariasis should be therefore considered as endemic in this area where it may represent a significant risk factor for public health.

No MeSH data available.


Related in: MedlinePlus

Histological section of subcutaneous formation extracted from hypogastrium (PAS staining; Case 1). a Section of the nodule containing the coiled worm (arrowheads) with visible genital tube (g), intestine (i), coelomyar musculature (m) and striated cuticle (c); 100 x. b Detailed view on longitudinal and transversal striation of the cuticle (arrowhead); 400 x
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4837637&req=5

Fig1: Histological section of subcutaneous formation extracted from hypogastrium (PAS staining; Case 1). a Section of the nodule containing the coiled worm (arrowheads) with visible genital tube (g), intestine (i), coelomyar musculature (m) and striated cuticle (c); 100 x. b Detailed view on longitudinal and transversal striation of the cuticle (arrowhead); 400 x

Mentions: Histological examination of the extracted tissue revealed layered structure with massive peripheral accumulation of inflammatory (mostly eosinophil) cells with surrounded central space with a coiled roundworm sized 600–1000 μm in diameter. The worm cuticle showed transversal striation and longitudinal ridges arranged evenly with wide inter-ridge spaces exceeding their height. The worm musculature was that of coelomyarian type; the intestine and reproduction tube had parenchymatous tissue in its lumen (Fig. 1). Based on morphological features including the distribution of longitudinal ridges, the worm was determined as a larval/subadult worm of Dirofilaria, most likely of D. repens. Using DNA analysis, the D. repens was confirmed. Increased anti-filaria IgG antibodies levels were detected in blood samples obtained 1 and 2 months post extirpation; neither eosinophilia nor increased total IgE levels pointing to ongoing helminthiasis were measured.Fig. 1


Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).

Matějů J, Chanová M, Modrý D, Mitková B, Hrazdilová K, Žampachová V, Kolářová L - BMC Infect. Dis. (2016)

Histological section of subcutaneous formation extracted from hypogastrium (PAS staining; Case 1). a Section of the nodule containing the coiled worm (arrowheads) with visible genital tube (g), intestine (i), coelomyar musculature (m) and striated cuticle (c); 100 x. b Detailed view on longitudinal and transversal striation of the cuticle (arrowhead); 400 x
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837637&req=5

Fig1: Histological section of subcutaneous formation extracted from hypogastrium (PAS staining; Case 1). a Section of the nodule containing the coiled worm (arrowheads) with visible genital tube (g), intestine (i), coelomyar musculature (m) and striated cuticle (c); 100 x. b Detailed view on longitudinal and transversal striation of the cuticle (arrowhead); 400 x
Mentions: Histological examination of the extracted tissue revealed layered structure with massive peripheral accumulation of inflammatory (mostly eosinophil) cells with surrounded central space with a coiled roundworm sized 600–1000 μm in diameter. The worm cuticle showed transversal striation and longitudinal ridges arranged evenly with wide inter-ridge spaces exceeding their height. The worm musculature was that of coelomyarian type; the intestine and reproduction tube had parenchymatous tissue in its lumen (Fig. 1). Based on morphological features including the distribution of longitudinal ridges, the worm was determined as a larval/subadult worm of Dirofilaria, most likely of D. repens. Using DNA analysis, the D. repens was confirmed. Increased anti-filaria IgG antibodies levels were detected in blood samples obtained 1 and 2 months post extirpation; neither eosinophilia nor increased total IgE levels pointing to ongoing helminthiasis were measured.Fig. 1

Bottom Line: In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans.At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad).The findings confirm the natural setting of D. repens in South Moravian Region of the CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic.

ABSTRACT

Background: Human dirofilariasis is a zoonotic infection that continues to spread to previously unaffected areas of Europe. In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans. In the present paper, human Dirofilaria infections are reported from the country for the first time.

Case presentation: The samples from five patients with suspected tissue helminthiases were investigated. In particular cases, nematodes were isolated from various tissues including skin of lower leg, soft tissues of finger, subcutaneous tissue of hypogastrium, lymph node and peritoneum. The diagnosis was based on light microscopic morphology and/or DNA analysis of the worms. In addition, ELISA examination of patients' sera for anti-filaria IgG antibodies was performed.

Conclusions: In the CR, five cases of human dirofilariasis caused by Dirofilaria repens were recorded during 2010-2014 (species determination for three of them was confirmed besides morphological also by DNA analysis). At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad). The findings confirm the natural setting of D. repens in South Moravian Region of the CR. Dirofilariasis should be therefore considered as endemic in this area where it may represent a significant risk factor for public health.

No MeSH data available.


Related in: MedlinePlus