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MicroRNA-138 functions as a tumor suppressor in osteosarcoma by targeting differentiated embryonic chondrocyte gene 2.

Jiang B, Mu W, Wang J, Lu J, Jiang S, Li L, Xu H, Tian H - J. Exp. Clin. Cancer Res. (2016)

Bottom Line: MicroRNA-138 (miR-138) has been proven to be a tumor suppressor gene in various types of tumors.Moreover, miR-138 expression was significantly lower in metastatic osteosarcoma tissues than that in non-metastatic tissues.DEC2 was verified as a direct target of miR-138, and DEC2 could reverse the inhibitory effect of miR-138 on osteosarcoma progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Traumatic Orthopaedics, The People 's Hospital of Dongying City of Shandong Province, No 317 Nanyi Road, Dongying, 257091, Shandong, China. baoen_jiang@163.com.

ABSTRACT

Background: MicroRNA-138 (miR-138) has been proven to be a tumor suppressor gene in various types of tumors. However, the expression and the role of miR-138 in human osteosarcoma are still poorly understood. We investigated the function and the underlying mechanism of miR-138 in osteosarcoma.

Methods: The expression of miR-138 in human osteosarcoma tissues and cell lines was detected by real-time PCR analysis. The gain-of-function and loss-of-function experiments were performed on osteosarcoma cell lines to investigate the effects of miR-138 on osteosarcoma progression, and to determine whether differentiated embryonic chondrocyte gene 2 (DEC2) mediates these effects. Cell proliferation, apoptosis and invasion were assessed by MTT, flow cytometry and transwell-matrigel assays. Dual-luciferase reporter assay was used to identify whether DEC2 is a direct target of miR-138.

Results: MiR-138 was significantly downregulated in human osteosarcoma tissues and cell lines. Moreover, miR-138 expression was significantly lower in metastatic osteosarcoma tissues than that in non-metastatic tissues. The in vitro gain-of-function and loss-of-function experiments demonstrated that miR-138 inhibited cell proliferation and invasion, and promoted cell apoptosis of human osteosarcoma cells. DEC2 was verified as a direct target of miR-138, and DEC2 could reverse the inhibitory effect of miR-138 on osteosarcoma progression.

Conclusions: These findings suggested that miR-138 acts as a tumor suppressor in osteosarcoma.miR-138 inhibited cell proliferation and invasion, as well as promoted cell apoptosis of human osteosarcoma cells, at least partially, by inhibiting the expression of DEC2. MiR-138/DEC2 may be a novel therapeutic target in osteosarcoma.

No MeSH data available.


Related in: MedlinePlus

Mir-138 expression was downregulated in human osteosarcoma tissues and cell lines. a Fold change of miR-138 expression in human osteosarcoma tissues and the adjacent normal tissues. #P < 0.01 compared with the normal. b Relative expression of miR-138 in the osteosarcoma tissues from the patients with metastases and non-metastases. #P < 0.01 compared with the non-metastases tissues. c Fold change of miR-138 expression in human osteosarcoma cell lines (MG-63, U2OS, SJSA-1, and SAOS-2) and the normal bone cell line (hFOB). #P < 0.01 compared with hFOB
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Fig1: Mir-138 expression was downregulated in human osteosarcoma tissues and cell lines. a Fold change of miR-138 expression in human osteosarcoma tissues and the adjacent normal tissues. #P < 0.01 compared with the normal. b Relative expression of miR-138 in the osteosarcoma tissues from the patients with metastases and non-metastases. #P < 0.01 compared with the non-metastases tissues. c Fold change of miR-138 expression in human osteosarcoma cell lines (MG-63, U2OS, SJSA-1, and SAOS-2) and the normal bone cell line (hFOB). #P < 0.01 compared with hFOB

Mentions: The expression level of miR-138 was quantified by real-time quantitative reverse transcription PCR in primary osteosarcoma tissues and osteosarcoma cell lines. Results showed that compared with the adjacent normal tissues, the expression level of miR-138 was significantly downregulated in human osteosarcoma tissues (Fig. 1a, P < 0.01). Moreover, miR-138 expression was significantly lower in metastatic osteosarcoma tissues than that in non-metastatic tissues (Fig. 1b, P < 0.01). Compared with the normal bone cell line hFOB, the expression levels of miR-138 were significantly downregulated in osteosarcoma cell lines, including MG-63, U2OS, SJSA-1, and Saos-2 (Fig. 1c, P < 0.01).Fig 1


MicroRNA-138 functions as a tumor suppressor in osteosarcoma by targeting differentiated embryonic chondrocyte gene 2.

Jiang B, Mu W, Wang J, Lu J, Jiang S, Li L, Xu H, Tian H - J. Exp. Clin. Cancer Res. (2016)

Mir-138 expression was downregulated in human osteosarcoma tissues and cell lines. a Fold change of miR-138 expression in human osteosarcoma tissues and the adjacent normal tissues. #P < 0.01 compared with the normal. b Relative expression of miR-138 in the osteosarcoma tissues from the patients with metastases and non-metastases. #P < 0.01 compared with the non-metastases tissues. c Fold change of miR-138 expression in human osteosarcoma cell lines (MG-63, U2OS, SJSA-1, and SAOS-2) and the normal bone cell line (hFOB). #P < 0.01 compared with hFOB
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837633&req=5

Fig1: Mir-138 expression was downregulated in human osteosarcoma tissues and cell lines. a Fold change of miR-138 expression in human osteosarcoma tissues and the adjacent normal tissues. #P < 0.01 compared with the normal. b Relative expression of miR-138 in the osteosarcoma tissues from the patients with metastases and non-metastases. #P < 0.01 compared with the non-metastases tissues. c Fold change of miR-138 expression in human osteosarcoma cell lines (MG-63, U2OS, SJSA-1, and SAOS-2) and the normal bone cell line (hFOB). #P < 0.01 compared with hFOB
Mentions: The expression level of miR-138 was quantified by real-time quantitative reverse transcription PCR in primary osteosarcoma tissues and osteosarcoma cell lines. Results showed that compared with the adjacent normal tissues, the expression level of miR-138 was significantly downregulated in human osteosarcoma tissues (Fig. 1a, P < 0.01). Moreover, miR-138 expression was significantly lower in metastatic osteosarcoma tissues than that in non-metastatic tissues (Fig. 1b, P < 0.01). Compared with the normal bone cell line hFOB, the expression levels of miR-138 were significantly downregulated in osteosarcoma cell lines, including MG-63, U2OS, SJSA-1, and Saos-2 (Fig. 1c, P < 0.01).Fig 1

Bottom Line: MicroRNA-138 (miR-138) has been proven to be a tumor suppressor gene in various types of tumors.Moreover, miR-138 expression was significantly lower in metastatic osteosarcoma tissues than that in non-metastatic tissues.DEC2 was verified as a direct target of miR-138, and DEC2 could reverse the inhibitory effect of miR-138 on osteosarcoma progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Traumatic Orthopaedics, The People 's Hospital of Dongying City of Shandong Province, No 317 Nanyi Road, Dongying, 257091, Shandong, China. baoen_jiang@163.com.

ABSTRACT

Background: MicroRNA-138 (miR-138) has been proven to be a tumor suppressor gene in various types of tumors. However, the expression and the role of miR-138 in human osteosarcoma are still poorly understood. We investigated the function and the underlying mechanism of miR-138 in osteosarcoma.

Methods: The expression of miR-138 in human osteosarcoma tissues and cell lines was detected by real-time PCR analysis. The gain-of-function and loss-of-function experiments were performed on osteosarcoma cell lines to investigate the effects of miR-138 on osteosarcoma progression, and to determine whether differentiated embryonic chondrocyte gene 2 (DEC2) mediates these effects. Cell proliferation, apoptosis and invasion were assessed by MTT, flow cytometry and transwell-matrigel assays. Dual-luciferase reporter assay was used to identify whether DEC2 is a direct target of miR-138.

Results: MiR-138 was significantly downregulated in human osteosarcoma tissues and cell lines. Moreover, miR-138 expression was significantly lower in metastatic osteosarcoma tissues than that in non-metastatic tissues. The in vitro gain-of-function and loss-of-function experiments demonstrated that miR-138 inhibited cell proliferation and invasion, and promoted cell apoptosis of human osteosarcoma cells. DEC2 was verified as a direct target of miR-138, and DEC2 could reverse the inhibitory effect of miR-138 on osteosarcoma progression.

Conclusions: These findings suggested that miR-138 acts as a tumor suppressor in osteosarcoma.miR-138 inhibited cell proliferation and invasion, as well as promoted cell apoptosis of human osteosarcoma cells, at least partially, by inhibiting the expression of DEC2. MiR-138/DEC2 may be a novel therapeutic target in osteosarcoma.

No MeSH data available.


Related in: MedlinePlus