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Urine proteomics in the diagnosis of stable angina.

Neisius U, Koeck T, Mischak H, Rossi SH, Olson E, Carty DM, Dymott JA, Dominiczak AF, Berry C, Oldroyd KG, Delles C - BMC Cardiovasc Disord (2016)

Bottom Line: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001).In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

View Article: PubMed Central - PubMed

Affiliation: BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.

ABSTRACT

Background: We have previously described a panel of 238 urinary polypeptides specific for established severe coronary artery disease (CAD). Here we studied this polypeptide panel in patients with a wider range of CAD severity.

Methods: We recruited 60 patients who underwent elective coronary angiography for investigation of stable angina. Patients were selected for either having angiographic evidence of CAD or not (NCA) following coronary angiography (n = 30/30; age, 55 ± 6 vs. 56 ± 7 years, P = 0.539) to cover the extremes of the CAD spectrum. A further 66 patients with severe CAD (age, 64 ± 9 years) prior to surgical coronary revascularization were added for correlation studies. The Gensini score was calculated from coronary angiograms as a measure of CAD severity. Urinary proteomic analyses were performed using capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. The urinary polypeptide pattern was classified using a predefined algorithm and resulting in the CAD238 score, which expresses the pattern quantitatively.

Results: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001). After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001). In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).

Conclusions: In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

No MeSH data available.


Related in: MedlinePlus

Correlation between CAD238 score and the Gensini score. The Gensini score (y-axis) is plotted against the CAD238 score (x-axis) for 96 patients. Shown are the Spearman’s correlation coefficient and the corresponding P-value
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Fig2: Correlation between CAD238 score and the Gensini score. The Gensini score (y-axis) is plotted against the CAD238 score (x-axis) for 96 patients. Shown are the Spearman’s correlation coefficient and the corresponding P-value

Mentions: The Gensini score was different in patients with stable angina and CAD compared to CAD patients undergoing CABG (40 [25; 61] vs. 77 [56; 109]; P < 0.001). When all patients with CAD were combined (n = 96) the CAD238 score correlated closely with the Gensini score (ρ = 0.465, P < 0.001; Fig. 2). Due to the difference in age between patients with CAD and stable angina and those prior to surgical coronary revascularization (55.1 ± 6.0 vs. 64.3 ± 8.8 years, P < 0.001), we adjusted for age using a linear regression model. After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001).Fig. 2


Urine proteomics in the diagnosis of stable angina.

Neisius U, Koeck T, Mischak H, Rossi SH, Olson E, Carty DM, Dymott JA, Dominiczak AF, Berry C, Oldroyd KG, Delles C - BMC Cardiovasc Disord (2016)

Correlation between CAD238 score and the Gensini score. The Gensini score (y-axis) is plotted against the CAD238 score (x-axis) for 96 patients. Shown are the Spearman’s correlation coefficient and the corresponding P-value
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837614&req=5

Fig2: Correlation between CAD238 score and the Gensini score. The Gensini score (y-axis) is plotted against the CAD238 score (x-axis) for 96 patients. Shown are the Spearman’s correlation coefficient and the corresponding P-value
Mentions: The Gensini score was different in patients with stable angina and CAD compared to CAD patients undergoing CABG (40 [25; 61] vs. 77 [56; 109]; P < 0.001). When all patients with CAD were combined (n = 96) the CAD238 score correlated closely with the Gensini score (ρ = 0.465, P < 0.001; Fig. 2). Due to the difference in age between patients with CAD and stable angina and those prior to surgical coronary revascularization (55.1 ± 6.0 vs. 64.3 ± 8.8 years, P < 0.001), we adjusted for age using a linear regression model. After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001).Fig. 2

Bottom Line: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001).In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

View Article: PubMed Central - PubMed

Affiliation: BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.

ABSTRACT

Background: We have previously described a panel of 238 urinary polypeptides specific for established severe coronary artery disease (CAD). Here we studied this polypeptide panel in patients with a wider range of CAD severity.

Methods: We recruited 60 patients who underwent elective coronary angiography for investigation of stable angina. Patients were selected for either having angiographic evidence of CAD or not (NCA) following coronary angiography (n = 30/30; age, 55 ± 6 vs. 56 ± 7 years, P = 0.539) to cover the extremes of the CAD spectrum. A further 66 patients with severe CAD (age, 64 ± 9 years) prior to surgical coronary revascularization were added for correlation studies. The Gensini score was calculated from coronary angiograms as a measure of CAD severity. Urinary proteomic analyses were performed using capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. The urinary polypeptide pattern was classified using a predefined algorithm and resulting in the CAD238 score, which expresses the pattern quantitatively.

Results: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001). After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001). In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).

Conclusions: In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

No MeSH data available.


Related in: MedlinePlus