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Urine proteomics in the diagnosis of stable angina.

Neisius U, Koeck T, Mischak H, Rossi SH, Olson E, Carty DM, Dymott JA, Dominiczak AF, Berry C, Oldroyd KG, Delles C - BMC Cardiovasc Disord (2016)

Bottom Line: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001).In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

View Article: PubMed Central - PubMed

Affiliation: BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.

ABSTRACT

Background: We have previously described a panel of 238 urinary polypeptides specific for established severe coronary artery disease (CAD). Here we studied this polypeptide panel in patients with a wider range of CAD severity.

Methods: We recruited 60 patients who underwent elective coronary angiography for investigation of stable angina. Patients were selected for either having angiographic evidence of CAD or not (NCA) following coronary angiography (n = 30/30; age, 55 ± 6 vs. 56 ± 7 years, P = 0.539) to cover the extremes of the CAD spectrum. A further 66 patients with severe CAD (age, 64 ± 9 years) prior to surgical coronary revascularization were added for correlation studies. The Gensini score was calculated from coronary angiograms as a measure of CAD severity. Urinary proteomic analyses were performed using capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. The urinary polypeptide pattern was classified using a predefined algorithm and resulting in the CAD238 score, which expresses the pattern quantitatively.

Results: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001). After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001). In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).

Conclusions: In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

No MeSH data available.


Related in: MedlinePlus

CAD238 score comparison between patients with NCA and CAD. Lines represent the mean. NCA normal coronary arteries, CAD coronary artery disease
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Fig1: CAD238 score comparison between patients with NCA and CAD. Lines represent the mean. NCA normal coronary arteries, CAD coronary artery disease

Mentions: Cardiovascular risk factors were similar in patients with or without angiographic evidence of CAD (Table 1). Similarly, in patients with angina like chest pain the CAD238 score was not significantly different between patients with CAD and those with NCA (−0.487 ± 0.341 vs. −0.612 ± 0.269, P = 0.119) as shown in Fig. 1. To adjust for potential cofounding factors we used a stepwise linear regression model with CAD238 score, age, gender and diabetes status as predictors of CAD. The resulting model contained only the CAD238 score (β = 0.206; P = 0.090) and gender (β = 0.128; P = 0.092), but remained statistically non-significant (P = 0.072).Table 1


Urine proteomics in the diagnosis of stable angina.

Neisius U, Koeck T, Mischak H, Rossi SH, Olson E, Carty DM, Dymott JA, Dominiczak AF, Berry C, Oldroyd KG, Delles C - BMC Cardiovasc Disord (2016)

CAD238 score comparison between patients with NCA and CAD. Lines represent the mean. NCA normal coronary arteries, CAD coronary artery disease
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837614&req=5

Fig1: CAD238 score comparison between patients with NCA and CAD. Lines represent the mean. NCA normal coronary arteries, CAD coronary artery disease
Mentions: Cardiovascular risk factors were similar in patients with or without angiographic evidence of CAD (Table 1). Similarly, in patients with angina like chest pain the CAD238 score was not significantly different between patients with CAD and those with NCA (−0.487 ± 0.341 vs. −0.612 ± 0.269, P = 0.119) as shown in Fig. 1. To adjust for potential cofounding factors we used a stepwise linear regression model with CAD238 score, age, gender and diabetes status as predictors of CAD. The resulting model contained only the CAD238 score (β = 0.206; P = 0.090) and gender (β = 0.128; P = 0.092), but remained statistically non-significant (P = 0.072).Table 1

Bottom Line: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001).In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

View Article: PubMed Central - PubMed

Affiliation: BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.

ABSTRACT

Background: We have previously described a panel of 238 urinary polypeptides specific for established severe coronary artery disease (CAD). Here we studied this polypeptide panel in patients with a wider range of CAD severity.

Methods: We recruited 60 patients who underwent elective coronary angiography for investigation of stable angina. Patients were selected for either having angiographic evidence of CAD or not (NCA) following coronary angiography (n = 30/30; age, 55 ± 6 vs. 56 ± 7 years, P = 0.539) to cover the extremes of the CAD spectrum. A further 66 patients with severe CAD (age, 64 ± 9 years) prior to surgical coronary revascularization were added for correlation studies. The Gensini score was calculated from coronary angiograms as a measure of CAD severity. Urinary proteomic analyses were performed using capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. The urinary polypeptide pattern was classified using a predefined algorithm and resulting in the CAD238 score, which expresses the pattern quantitatively.

Results: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001). After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001). In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119).

Conclusions: In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

No MeSH data available.


Related in: MedlinePlus