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Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells.

Tabatabaei FS, Torshabi M - J Oral Maxillofac Res (2016)

Bottom Line: The dentin proteins were extracted and purified.However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05).The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical sciencesIran.; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesIran.

ABSTRACT

Objectives: The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1.

Material and methods: The dental pulp cells were isolated and cultured. The dentin proteins were extracted and purified. The MTT assay was performed for assessment of cell viability and proliferation in the presence of different concentrations of dentin proteins and growth factors during 24 - 72 h post-treatment.

Results: The cells treated with 250 ng/mL dentin proteins had the best viability and proliferation ability in comparison with other concentrations (P < 0.05). The MTT assay demonstrated that cells cultured with 5 ng/mL platelet-derived growth factor BB had the highest viability at each time point as compared to other groups (P < 0.05). However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05). The least viability and proliferation at each growth factor concentration was seen in cells treated with combination of transforming growth factor beta 1 and dentin proteins at 72 h (P < 0.05).

Conclusions: The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells.

No MeSH data available.


Related in: MedlinePlus

The effect of TGF-β1 and PDGF-BB, and their combinations with DNCPs (250 ng/ml) on cell viability in 3 time intervals assessed by MTT cell proliferation assay.A = 5 ng/mL; B = 10 ng/mL.aStatistically significant difference at the level P < 0.05 (Tukey’s post-hoc test) compared with the control group (regular medium without DNCPs, TGF-β1 and PDGF-BB; viability 100%).
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fig2: The effect of TGF-β1 and PDGF-BB, and their combinations with DNCPs (250 ng/ml) on cell viability in 3 time intervals assessed by MTT cell proliferation assay.A = 5 ng/mL; B = 10 ng/mL.aStatistically significant difference at the level P < 0.05 (Tukey’s post-hoc test) compared with the control group (regular medium without DNCPs, TGF-β1 and PDGF-BB; viability 100%).

Mentions: As seen in Figure 2A, DNCPs in presence of 5 ng/mL TGF-β1 showed a significantly higher viability (133.9 [15.4], P < 0.05) at 24 h compared to the control group (Regular medium only). However, no significant differences were noted in this regard with the control group at 48 or 72 h (P > 0.05). Although combination of 5 ng/mL TGF-β1 and 250 ng/mL DNCPs caused no significant change in cell viability compared to the control group at 24 and 48 h, it significantly decreased cell viability at 72 h (71 [11.6], P < 0.05). Also as time passed, the significant reduction (~20%) in cell viability (72 vs 24 h) indicated that cell proliferation rate decreased during 24 - 72 h post-treatment in this group.


Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells.

Tabatabaei FS, Torshabi M - J Oral Maxillofac Res (2016)

The effect of TGF-β1 and PDGF-BB, and their combinations with DNCPs (250 ng/ml) on cell viability in 3 time intervals assessed by MTT cell proliferation assay.A = 5 ng/mL; B = 10 ng/mL.aStatistically significant difference at the level P < 0.05 (Tukey’s post-hoc test) compared with the control group (regular medium without DNCPs, TGF-β1 and PDGF-BB; viability 100%).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837608&req=5

fig2: The effect of TGF-β1 and PDGF-BB, and their combinations with DNCPs (250 ng/ml) on cell viability in 3 time intervals assessed by MTT cell proliferation assay.A = 5 ng/mL; B = 10 ng/mL.aStatistically significant difference at the level P < 0.05 (Tukey’s post-hoc test) compared with the control group (regular medium without DNCPs, TGF-β1 and PDGF-BB; viability 100%).
Mentions: As seen in Figure 2A, DNCPs in presence of 5 ng/mL TGF-β1 showed a significantly higher viability (133.9 [15.4], P < 0.05) at 24 h compared to the control group (Regular medium only). However, no significant differences were noted in this regard with the control group at 48 or 72 h (P > 0.05). Although combination of 5 ng/mL TGF-β1 and 250 ng/mL DNCPs caused no significant change in cell viability compared to the control group at 24 and 48 h, it significantly decreased cell viability at 72 h (71 [11.6], P < 0.05). Also as time passed, the significant reduction (~20%) in cell viability (72 vs 24 h) indicated that cell proliferation rate decreased during 24 - 72 h post-treatment in this group.

Bottom Line: The dentin proteins were extracted and purified.However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05).The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical sciencesIran.; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesIran.

ABSTRACT

Objectives: The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1.

Material and methods: The dental pulp cells were isolated and cultured. The dentin proteins were extracted and purified. The MTT assay was performed for assessment of cell viability and proliferation in the presence of different concentrations of dentin proteins and growth factors during 24 - 72 h post-treatment.

Results: The cells treated with 250 ng/mL dentin proteins had the best viability and proliferation ability in comparison with other concentrations (P < 0.05). The MTT assay demonstrated that cells cultured with 5 ng/mL platelet-derived growth factor BB had the highest viability at each time point as compared to other groups (P < 0.05). However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05). The least viability and proliferation at each growth factor concentration was seen in cells treated with combination of transforming growth factor beta 1 and dentin proteins at 72 h (P < 0.05).

Conclusions: The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells.

No MeSH data available.


Related in: MedlinePlus