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Clinical, Radiographic, and Histologic Evaluation of Maxillary Sinus Lift Procedure Using a Highly Purified Xenogenic Graft (Laddec(®)).

Guarnieri R, Belleggia F, Ippoliti S, DeVilliers P, Stefanelli LV, Di Carlo S, Pompa G - J Oral Maxillofac Res (2016)

Bottom Line: The original height was 3.65 (SD 0.7) mm and the augmented sinus height was 13.8 (SD 1.4) mm after the surgery.The reduced height of grafted xenogenic material (RDL) at the implant insertion was 0.83 (SD 0.38) mm, and at the final postoperative visit was 0.91 (SD 0.25) mm, showing no significant correlation with the follow-up periods by Spearman's test (P = 0.118).In addition, no significant difference in the RDL was observed according to the site of implantation (P = 0.682).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Dental and Maxillofacial Sciences, School of Dentistry, University La Sapienza, Roma Italy.

ABSTRACT

Objectives: The aim of this study was to evaluate the clinical, radiographic and histologic results when a highly purified xenogenic bone (Laddec(®)) was used as grafting material in maxillary sinuses.

Material and methods: In fifteen patients requiring unilateral maxillary sinus augmentation, the grafting procedure was performed with Laddec(®). Forty-two implants were installed after a 6 month healing period. The height of the augmented sinus was measured radiographically immediately after augmentation and postoperatively up to 36 months. At the time of implant placement, a bone core was harvested in each patient for histological examination.

Results: The cumulative implant survival rate was 97.6%. The original height was 3.65 (SD 0.7) mm and the augmented sinus height was 13.8 (SD 1.4) mm after the surgery. The reduced height of grafted xenogenic material (RDL) at the implant insertion was 0.83 (SD 0.38) mm, and at the final postoperative visit was 0.91 (SD 0.25) mm, showing no significant correlation with the follow-up periods by Spearman's test (P = 0.118). In addition, no significant difference in the RDL was observed according to the site of implantation (P = 0.682). The mean implant marginal bone loss was 0.38 (SD 0.24) mm. Histological analysis showed the bone cores were composed of 64.72 (SD 3.44)% newly formed bone, 17.41 (SD 2.02)% connective tissue, 16.93 (SD 2.83)% residual graft particles, and 0.94 (SD 0.11)% inflammatory cells.

Conclusions: According to our data, the highly purified xenogenic bone (Laddec(®)), used as graft material in the sinus lift procedure, may create adequate bone volume, and appropriate osseointegration of dental implants.

No MeSH data available.


Related in: MedlinePlus

Trichrome stain of the osteoid rim and graft material. A = original magnification x20; B = original magnification x100.Red osteoid rim (yellow arrows) green viable bone (blue arrows) and graft material (red arrows).
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fig11: Trichrome stain of the osteoid rim and graft material. A = original magnification x20; B = original magnification x100.Red osteoid rim (yellow arrows) green viable bone (blue arrows) and graft material (red arrows).

Mentions: At time of the implant surgery, bone cores were harvested using a 3.5 x 10 mm diameter trephine under cold (4 - 5 °C) sterile saline solution irrigation. The bone specimens were immediately fixed in 10% buffered formalin and embedded in a glycolmethacrylate resin. After polymerization, specimens were sectioned along their longitudinal axis to a thickness of 70 microns (Laddec® plastic Microtome, RM 2265). Slides were stained with trichrome, methilyne blue, and Von Kossa (Figure 10). Slides were examined using an Olympus B51 microscope. The histomorphometry was performed using Bioquant® image analysis software (R&M Biometrics, Nashville, TN, USA) and images were captured with a Q-Imaging camera, 32-0013B-157, RETIGA, Colour 12-bit (Figures 11 and 12).


Clinical, Radiographic, and Histologic Evaluation of Maxillary Sinus Lift Procedure Using a Highly Purified Xenogenic Graft (Laddec(®)).

Guarnieri R, Belleggia F, Ippoliti S, DeVilliers P, Stefanelli LV, Di Carlo S, Pompa G - J Oral Maxillofac Res (2016)

Trichrome stain of the osteoid rim and graft material. A = original magnification x20; B = original magnification x100.Red osteoid rim (yellow arrows) green viable bone (blue arrows) and graft material (red arrows).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837607&req=5

fig11: Trichrome stain of the osteoid rim and graft material. A = original magnification x20; B = original magnification x100.Red osteoid rim (yellow arrows) green viable bone (blue arrows) and graft material (red arrows).
Mentions: At time of the implant surgery, bone cores were harvested using a 3.5 x 10 mm diameter trephine under cold (4 - 5 °C) sterile saline solution irrigation. The bone specimens were immediately fixed in 10% buffered formalin and embedded in a glycolmethacrylate resin. After polymerization, specimens were sectioned along their longitudinal axis to a thickness of 70 microns (Laddec® plastic Microtome, RM 2265). Slides were stained with trichrome, methilyne blue, and Von Kossa (Figure 10). Slides were examined using an Olympus B51 microscope. The histomorphometry was performed using Bioquant® image analysis software (R&M Biometrics, Nashville, TN, USA) and images were captured with a Q-Imaging camera, 32-0013B-157, RETIGA, Colour 12-bit (Figures 11 and 12).

Bottom Line: The original height was 3.65 (SD 0.7) mm and the augmented sinus height was 13.8 (SD 1.4) mm after the surgery.The reduced height of grafted xenogenic material (RDL) at the implant insertion was 0.83 (SD 0.38) mm, and at the final postoperative visit was 0.91 (SD 0.25) mm, showing no significant correlation with the follow-up periods by Spearman's test (P = 0.118).In addition, no significant difference in the RDL was observed according to the site of implantation (P = 0.682).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Dental and Maxillofacial Sciences, School of Dentistry, University La Sapienza, Roma Italy.

ABSTRACT

Objectives: The aim of this study was to evaluate the clinical, radiographic and histologic results when a highly purified xenogenic bone (Laddec(®)) was used as grafting material in maxillary sinuses.

Material and methods: In fifteen patients requiring unilateral maxillary sinus augmentation, the grafting procedure was performed with Laddec(®). Forty-two implants were installed after a 6 month healing period. The height of the augmented sinus was measured radiographically immediately after augmentation and postoperatively up to 36 months. At the time of implant placement, a bone core was harvested in each patient for histological examination.

Results: The cumulative implant survival rate was 97.6%. The original height was 3.65 (SD 0.7) mm and the augmented sinus height was 13.8 (SD 1.4) mm after the surgery. The reduced height of grafted xenogenic material (RDL) at the implant insertion was 0.83 (SD 0.38) mm, and at the final postoperative visit was 0.91 (SD 0.25) mm, showing no significant correlation with the follow-up periods by Spearman's test (P = 0.118). In addition, no significant difference in the RDL was observed according to the site of implantation (P = 0.682). The mean implant marginal bone loss was 0.38 (SD 0.24) mm. Histological analysis showed the bone cores were composed of 64.72 (SD 3.44)% newly formed bone, 17.41 (SD 2.02)% connective tissue, 16.93 (SD 2.83)% residual graft particles, and 0.94 (SD 0.11)% inflammatory cells.

Conclusions: According to our data, the highly purified xenogenic bone (Laddec(®)), used as graft material in the sinus lift procedure, may create adequate bone volume, and appropriate osseointegration of dental implants.

No MeSH data available.


Related in: MedlinePlus