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Phage Peptide Libraries As a Source of Targeted Ligands.

Nemudraya AA, Richter VA, Kuligina EV - Acta Naturae (2016 Jan-Mar)

Bottom Line: One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs.This problem is particularly acute in relation to oncologic diseases.Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia.

ABSTRACT
One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs. This problem is particularly acute in relation to oncologic diseases. Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer. The review discusses in detail the technology of phage display as a method for obtaining specific targeted peptide agents and offers examples of their use in diagnostic and clinical practice.

No MeSH data available.


Related in: MedlinePlus

Schematic representation of a typical biopanning of a phage library. A,B – incubation of a combinatorial phage library with a target;C – washing-off of the unbound phages; D– elution of bound phages and their amplification for the nextrounds
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Figure 2: Schematic representation of a typical biopanning of a phage library. A,B – incubation of a combinatorial phage library with a target;C – washing-off of the unbound phages; D– elution of bound phages and their amplification for the nextrounds

Mentions: A typical biopanning round comprises the following steps: 1) incubation of acombinatorial phage library with the target (protein, cell culture, tumortissue, etc); 2) washing-off of unbound phages; 3) elution of bound phages; and4) amplification of the eluted phage for the next rounds(Fig. 2).


Phage Peptide Libraries As a Source of Targeted Ligands.

Nemudraya AA, Richter VA, Kuligina EV - Acta Naturae (2016 Jan-Mar)

Schematic representation of a typical biopanning of a phage library. A,B – incubation of a combinatorial phage library with a target;C – washing-off of the unbound phages; D– elution of bound phages and their amplification for the nextrounds
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837571&req=5

Figure 2: Schematic representation of a typical biopanning of a phage library. A,B – incubation of a combinatorial phage library with a target;C – washing-off of the unbound phages; D– elution of bound phages and their amplification for the nextrounds
Mentions: A typical biopanning round comprises the following steps: 1) incubation of acombinatorial phage library with the target (protein, cell culture, tumortissue, etc); 2) washing-off of unbound phages; 3) elution of bound phages; and4) amplification of the eluted phage for the next rounds(Fig. 2).

Bottom Line: One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs.This problem is particularly acute in relation to oncologic diseases.Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia.

ABSTRACT
One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs. This problem is particularly acute in relation to oncologic diseases. Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer. The review discusses in detail the technology of phage display as a method for obtaining specific targeted peptide agents and offers examples of their use in diagnostic and clinical practice.

No MeSH data available.


Related in: MedlinePlus